Lab Report Cell Counting Lab

The guiding question relates to our background info because in order to know the effect the different solution had on the cell size we had to know our terminology, which was included in the background information. Section2: For this lab we had to follow certain procedures.

My partner and I choose glucose, colchicine, cold temperature (0 degrees Celsius), and warm temperature (37 degrees Celsius) as the addition factors. The tetrahymena were exposed exposed to their condition for ten minutes before the India ink was placed in their microcentrifuge. The tertrahymena were fixed on a slide every ten minutes after the ink was placed in the microcentrifuge. In this experiment there are two controls. There is positive control and a negative control. The untreated sample is tetrahymena that did not have any other conditions besides the ink. Then before the sample was placed on the slide they were fixed. The treated sample was tetrahymen mixed with

b. Is pigment distribution uniformly in each mycelium? If not, where is the pigment concentrated in each mold?

This lab consisted of two parts over a span of three days. For part one on day one, we first began by determining the number of dilution that will be performed, and what the final dilution is going to be. My lab partner and I then disinfected our work bench to begin our procedure. To begin, using a filtered tip, we pipetted 900µL of sterile water into a labeled microcentrifuge tube for our 1:10 dilution. We then continued to keep diluting the tubes until we reached our final 1:1 dilution. We then vortexed our working stock for approximately 5 seconds and used a pipet to take 100 µL of the working stock solution to eject it into our first dilution microcentrifuge tube that contained 900 µL of sterile water. My lab partner and I used the same pipet to take out 100 µL of the first dilution to our next dilution that contained 900 µL of sterile water.

Observe: Wait until the numbers are not changing very much. What do you notice about the solute concentrations inside and outside of the cell? _______________________________

The dialysis tube was submerged in a beaker filled with water. Both dialysis tubes were rinsed by distilled H2O before placing them inside and left the cell model aside for one hour.

To get the results of this experiment I had to use the Lincoln-Peterson Equation, and the Percent Error equation. To find the estimate population size using the Lincoln-Peterson Equation I had to use the number given in the first attempt which was 20 tester organisms I captured, and the number of organisms I captured from the second attempt which was 18 organisms and out of those only 8 were testers.

The sixth lab I completed in Biology 101 taught me how autotrophs (self-feeders) and heterotrophs (other-feeders) make organic food molecules by using photosynthesis. Photosynthesis uses the energy from the sun and it is captured and stored in the chemical bonds of organic molecules. The sunlight consists of different wavelengths of light. In plant chloroplasts, they have different pigments that capture different wavelengths of light. Light capturing pigments in green plants are called chlorophylls and these absorb all the colors of light except green, which is mostly reflected. To separate molecules from each other according to their solubility in a particular solvent is done by the process of chromatography. This basically means that polar

E places the arm cuff on Ps non dominant hand (make sure the artery label is lined up with Ps artery)

Discussion The purpose of this experiment was to determine the optimal environment of RAW 264.7 cells, which includes nutrients, temperature, and growth factors. To observe the effect of these three factors on cell growth, cells were plated and cultured in a DMEM media, RPMI media, and FBS growth factor concentrations of 1%, 5%, and 10% at corresponding temperatures of 25°C, 37°C, and 40°C. The growth of the cell was quantified by Calcein fluorescence of the plates on the CytoFluor II plate reader. Data was analyzed by averaging the wells to measure averaged fluorescent units and obtaining relative fluorescents units by subtracting background fluorescence of 8000 for a 24-well plate from the averages fluorescent units. Organisms also need

Hemocytometer counts do not differentiate between living and dead cells. To accomplish this, strains are used to help make the distinction. When stains are used, the dead cells absorb the strain while viable cells exclude the stain. Viable cell counting is another method used to determine the number of yeast cells. Viable counting involved a sample of the culture of cells on an agar plate.

**1.1 – Diagram & Description of Cellular Components will be found in handwritten portion of assignment**

Cell movement involves both the interactions between signaling molecules and the structure of the cytoskeleton (Holmes et al., 2012). Tiny hair like structures referred to as cilia are located on the surface of eukaryotic cells. Cilia are most responsible for the movement of cells and can process external signals which coordinate the correct arrangement of the inner organs during the development of an organism. Approximately 600 different cilia proteins are synthesized inside a cell and then transported into cilium. Disruption of this transport referred to as intraflagellar transport can result in errors during the assembly of cilia (Planck, 2013). In order to fully understand cell motility one needs to know that cell movement deals with several types of motion, these include the movement of an entire cell through fluid or a macrophage crawling through tissues and engulfing foreign bacteria. Also, beating of cilia or flagella causes the movement of fluid past cells which are anchored to a solid surface (Mitchell, 2015). Even though eukaryotic cells can move in a variety of ways, the main component of motility includes protein filaments called the cytoskeleton. The cytoskeleton is composed of three types of filaments which include; microfilaments, microtubules, and intermediate filaments (Alberts et al., 2014). Although there are three types of filaments, this lab focuses

The Packed Cell Volume, also known as the Hematocrit, measures the percentage of red blood cells that occupy any unit volume of blood. Invert your blood tube two or three times to properly mix your blood before uncapping. Holding your microhematocrit tube in one hand, begin to tilt your blood tube in a pouring motion until the blood comes to the lip of the tube. Because of the nature of the tube, the blood should not drip out at this angle. Hold the hematocrit tube to the lip of the tube at a 30 ° angle or until it begins to fill with blood. Continue until the tube is roughly ¾ full. Recap your blood tube and press your hematocrit tube into the clay to seal one end.

Total hemocytes count: The number of cells was counted using a Bürker- Turk hemocytometer (Van der knap et al., 1981) by using 10 µl of hemolymph of each group.