My partner and I choose glucose, colchicine, cold temperature (0 degrees Celsius), and warm temperature (37 degrees Celsius) as the addition factors. The tetrahymena were exposed exposed to their condition for ten minutes before the India ink was placed in their microcentrifuge. The tertrahymena were fixed on a slide every ten minutes after the ink was placed in the microcentrifuge. In this experiment there are two controls. There is positive control and a negative control. The untreated sample is tetrahymena that did not have any other conditions besides the ink. Then before the sample was placed on the slide they were fixed. The treated sample was tetrahymen mixed with
Keva Harris 25 February 2016 Biology Lab 27-13 Survey of the Kingdom Fungi Question 1: a. Are hyphae apparent? Yes b. Are the cells motile? Yes Question 2: a. How many species of mold are on the bread? Five b. Is pigment distribution uniformly in each mycelium? If not,
The sixth lab I completed in Biology 101 taught me how autotrophs (self-feeders) and heterotrophs (other-feeders) make organic food molecules by using photosynthesis. Photosynthesis uses the energy from the sun and it is captured and stored in the chemical bonds of organic molecules. The sunlight consists of different wavelengths of light. In plant chloroplasts, they have different pigments that capture different wavelengths of light. Light capturing pigments in green plants are called chlorophylls and these absorb all the colors of light except green, which is mostly reflected. To separate molecules from each other according to their solubility in a particular solvent is done by the process of chromatography. This basically means that polar
Madeline Jaquez Methods For this experiment, we used two restriction enzymes called Ava II and Pvu II. Four tubes were needed for the experiment as well. Each microtubule was labeled accordingly, A, B, and C. Using Micropipettes, we added 2 microliters of pUC19 DNA into each tube. To make sure the DNA was on the bottom of the tube, we tapped each tube on the lab bench. Each tube had its own specific amount of different solutions added on, however the final volume for each tube was 30 microliters. Tube A had, 24 microliters of DI water, 3 microliters of the buffer, and 1 microliter of the Ava II enzyme. Tube B had, 24 microliters of DI water, 3 microliters of the buffer, and 1 microliter of the Pvu II enzyme. Tube C had, 23 microliters of DI Water, 3 microliters of the buffer, 1 microliter of Ava II, and 1 microliter of Pvu II. The last tube C had, 25 microliters of DI water, 3 microliter of the buffer, and no enzymes. Each solution was added according to how it is written here. After we finished adding the solutions to each tube, we tapped the tubes on the lab bench to make sure the
This lab consisted of two parts over a span of three days. For part one on day one, we first began by determining the number of dilution that will be performed, and what the final dilution is going to be. My lab partner and I then disinfected our work bench to begin our procedure. To begin, using a filtered tip, we pipetted 900µL of sterile water into a labeled microcentrifuge tube for our 1:10 dilution. We then continued to keep diluting the tubes until we reached our final 1:1 dilution. We then vortexed our working stock for approximately 5 seconds and used a pipet to take 100 µL of the working stock solution to eject it into our first dilution microcentrifuge tube that contained 900 µL of sterile water. My lab partner and I used the same pipet to take out 100 µL of the first dilution to our next dilution that contained 900 µL of sterile water.
The purpose of this lab was to purify and test a GFP protein via several laboratory methods for the purpose of purifying and testing the protein in SDS-PAGE. To purify the protein chromatography and gel electrophoresis were the methods used in the experiment. GFP in the samples were tested using an ultraviolet light. When GFP was found present the cell were transformed into a petri dish containing ampicillin and arabinose. The cells were then lysed and SDS-PAGE was used to test. The results from the SDS-PAGE were inconclusive revealing there was no GFP present in the samples two through four.
The cells are placed into a flask and are forced through a nozzle so small that they must pass through one by one. In the nozzle, the cells are vibrated at different frequencies to produce drops (3). The drops of cells are then scanned by a laser that is used to count and measure each cell. Separating populations of cells involves attaching antibody linked fluorescent dye to certain cells of interest (3). The information that is gathered from the sorting and measuring of the cells is evaluated by a computer. The final steps for the FACS include applying an electrical charge to the drops of cells (3). Before the drop of cell forms at the end of the nozzle, a charge is applied to the stream that will determine where the drop will go (3). Based on the charge, the drip is either moved left or right with electrodes or placed in to designated final tubes. Quantifying the FACS information involves displaying the information so we know how many cells of each color and charge were
Introduction The purpose of this lab was to complete a variety of tests that are involved in determining a complete blood count including; a red blood cell count (RBC count), a white blood cell count (WBC count), a white blood cell differential (WBC differential), hematocrit, hemoglobin, and to calculate the
Materials and Methods The first thing we had to do in this experiment was label the 12 test tubes as C1- C4, G1- G4, and P1-P4. We filled one cylinder with water, another with glucose, and another with protein. Then we took the cylinder containing water and using a pipet we added 5 ml of water in each test tube marked C1-C4. We then took the cylinder containing glucose and with using a pipet we added 5 ml of glucose in each test tube labeled G1-G4. In the other tubes, we took the cylinders that contained protein and using a pipet, we put 5 ml in the test tubes that were labeled P1-P4. After
In activity three, a total count for white blood cells was conducted using a sample slide with a blood smear. The slide was observed under microscope with the above data as the result. The normal percentages for each blood count are as follows (zukur):
Methods and materials The first experiment begun by filling a 600-ml beaker, almost to the top, with water. Next, a 10-ml graduated cylinder was filled to the top with water. Once water was added to the beaker and graduated cylinder, a thumb was placed over the top of the graduated cylinder. This would ensure that no water was let out and no bubbles were let into the graduated cylinder. Next, it was turned upside down and fully submerged into the beaker. Then, a U-shaped glass tube was attained. The short end of the glass tube was placed into the beaker with the tip inside of the graduated cylinder. Next, a 50-ml Erlenmeyer flask was received. After, 10-ml of substrate concentration and 10-ml of catalase/buffer solution were placed into the flask. A rubber stopper was then placed on the opening of the flask. After adding these, the flask was held at the neck and spun softly
Materials/Methods and Results-Haematology Practical Haemoglobin Estimation: To determine the amount of haemoglobin concentration in the patient’s blood, an anticoagulated blood sample from the patient and a healthy control, were lysed by converting haemoglobin into cyanmethaemoglobin. Potassium ferricyanide and potassium cyanide are used for this conversion to occur, and the absorbance of