Cell Membrane Lab Report

8. What type of membrane does the dialysis tubing represent? Give an example of this type of

Explain what happened to the blood cells at the various levels of concentration. Be sure to refer to the solutions as being hypotonic, hypertonic and isotonic.

Blood is a non-Newtonian fluid that contains many components. One such component is red blood cells. Due to the red blood cells having the tendency to clump together at low velocities, calcium chloride was added in order to cause a thrombus formation. The blood used in this experiment was sheep’s blood.

3. Explain your prediction for the effect Na+ Cl- might have on glucose transport. In other words, explain why you picked the choice that you did. How well did the results compare with your prediction?

Blood is a bodily fluid that transports oxygen and nutrients to the cells within the body. Blood has plasma that lets the different types of blood travel round the body. Plasma contains proteins that have different functions for the blood- clotting, transporting and defence organisms and osmotic organisations. The plasma carries the red blood cell which has a elastic membrane so it can fit through the small capillaries within the body. Red blood cells can be also known as erythrocytes they don’t have a nucleus when they are matured which gives a bigger space for oxygen, although as there is no nuclei the red blood cells can’t divide so they only live for around 120 days. Red blood cells gain their colour from haemoglobin, oxygenated blood which is known as arterial blood which flows through the arteries coming from the heart and

Introduction: The biological membranes are composed of phospholipid bilayers, each phospholipid with hydrophilic heads and hydrophobic tails, and proteins. This arrangement of the proteins and lipids produces a selectively permeable membrane. Many kinds of molecules surround or are contained within

You may use the lab manual, pre-lab lectures, and credible internet resources, however you may not use your cell bio lab classmates as a resource. You will most likely see this material again on the Final and I highly encourage you to work individually and seek help from myself or your TA. Plagiarism will result in an automatic zero.

At this point due to the fluid, wastes, and electrolytes being built up the blood would turn toxic and

diH20 acted as a negative control in the experiment, all cells, both in intrafollicular zone and in interfollicular zone stained purple. Cells stained brown in the negative control indicate non-specific staining.

The diffusion across a cell membrane is a process of passive and spontaneous net movement of small lipophilic molecules. The molecules move from a high concentration to a low concentrated region along the concentration gradient. The result being a point of equilibrium, this is where a random molecular motion continues but there is no longer any net movement. However, there are things that can affect the rate of diffusion, these being temperature, surface area, concentration, size of the molecule, permeability, diffusion distance and concentration difference. Osmosis is a type of diffusion as it is the movement of water molecules through a semipermeable membrane into a region of higher solute concentration. Equilibrium is reached when the solute concentration is equal on both sides. Water potential is measured in kiloPascals, it is the measuring of the concentration of free water molecules that are able to diffuse compared to pure water, which is 0 kilopascals. It is a measure of the tendency of free water molecules to diffuse from one place to another. The result being, the more free water molecules, the higher the Water Potential. However, Water potential is affected by two factors: pressure and the amount of solute.

The other type of artificial blood is more of a blood substitute as it is derived from either outdated bovine or human red blood cells. It is known as Hemoglobin Based Oxygen Carriers (HBOC), Hemoglobin which is the oxygen carrying protein molecule found in red blood cells is extracted from the obsolete red blood cells through ultrafiltration and purification. The Hemoglobin must undergo specific processes in an attempt to prevent the Hemoglobin from disassociating from its natural four-chain configuration (Fridey 3). There is numerous methods of chemically altering the Hemoglobin to increase the molecules size so it does not dissociate and break down. The two main processes of enlarging the

The findings of this experiment reinforced the hypothesis that the resting membrane potential is most influenced by the ion potassium. We were able to deduce this through the collection of a multitude of intracellular and extracellular recordings, such as the one shown below in Figure 1. This shows how this experiment was able to record every single resting membrane potential in all three different muscle groups under all six solutions.

The purpose of this experiment was to determine the effects of tonicity on a cell membrane using red blood cells, potato strips and three unknown solutions (A, B, C). First three slides were prepared containing RBC’s and unknown solutions A, B and C. A control slide was prepared only using RBC’s. After observing each slide under the microscope it was determined that unknown solution A was hypertonic because the RBC appeared to have shrunk. The RBC in unknown solution B appeared to be swollen, therefor, the tonicity of unknown solution B was hypotonic. Unknown solution C showed no change to the RBC shape, it was suggested that unknown solution C was isotonic. To confirm the tonicity

In a hypertonic solution, the red blood cells will shrink and be destroyed, as water diffuse out of them.

When RBCs are suspended in an isotonic solution, nothing should be observed as there will be no net water movement between the RBCs and the solution. Thus, when 200 µL of blood was added to 10mL isotonic saline (0,154 M NaCl), voltage of 0V is recorded. When RBCs are suspended in a hypertonic solution, for instance, 0.4 M NaCl, RBCs shrunk due to decrease in cell volume as water diffused out of the cells by osmosis. The protein concentration within the cells become greater and more light is scattered and a negative