Cell Phase And Stationary Phase

1251 WordsMar 14, 20166 Pages
Introduction Paper chromatography is a method used to separate colour soluble substances ink. There are two phases: mobile phase and stationary phase. The stationary phase is chromatography paper. The mobile phase is the solvent that travels up the stationary phase with the samples. In the process, a dot of sample ink is placed on the line of origin (Wikipedia, 2011). After the chromatography paper touch the solvent, the solvent will travel up slowly and separate the component. In paper chromatography, the attraction of soluble substances between the solvent and paper varies with more strongly attracted substances moving more slowly up the paper than those less strongly attracted to the paper (Refer to Figure 1). The Retention Factor (Rf) is the ratio of the distance travelled by the colour component to the distance travelled by the solvent. The focus of the experiment is to determine which pen that student used in the notes by using chromatography. In order to determine which pen is the one that student used, it is necessary to compare the colour components and Rf values of different pens. If the there is one pen that has similar colour components and Rf values with the sample’s colour components and Rf values, this means that pen is as same the student’s pen. The solvents used in the experiment was water, acetone and ethanol. Water is polar because electrons or negative charge are attracted by the highly electronegative oxygen atom, making the region around the oxygen
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