Cell Sheet Adhesion Of Porcine Heart Tissue After Transplant

Although tissue engineers and researchers have already succeeded with creating new skin, blood vessels, bone and cartilage, the more complex organs are difficult to reproduce because of their different functions. Researchers must also be concerned with the mechanisms of growing the tissue. For instance, the advantages and disadvantages are not yet clear for the length of time the cells should be exposed to the growth factors or the difference between growing the tissue outside the body or implanting the scaffold inside the body and letting the tissue grow there. Scaffolding and injectable polymers that form scaffolds in irregular areas (like bone fractures) are also being improved. Much more research is being conducted in order to grow the hearts, livers, breasts, kidneys, and other valuable organs that so many people need.

Tissue engineering is an emerging interdisciplinary field that uses principles from engineering, biology and chemistry in an effort towards tissue regeneration. The main draw of tissue engineering is the regeneration of a patient’s own tissues and organs free from low biofunctionality and poor biocompatibility and serious immune rejection. As medical care continues to improve and life expectancy continues to grow, organ shortages become more problematic.(Manufacturing living things) According to organdonor.gov, a patient is added to the waiting list every 10 minutes and an average of 18 people die everyday waiting for an organ donation. The “nirvana” of tissue engineering is to replace the need for organ donation altogether. This could be achieved using scaffolding from

This new research uses the scaffolding technique combined with stem cells to grow a heart. The new breakthrough was made using poor quality donated hearts. There were first stripped of heart tissue, leaving only the scaffolding of the heart. All of the tissue must be gone so that a potential patient won't risk rejection.

Stem cells have the ability to grow heart cells, which can further lead to new discoveries in medicine. A team of scientists researching stem cells “has grown the earliest form of human heart cells from embryonic stem cells and found a way to direct them into the three major cell types found in the human heart”. This demonstrates the morality of stem cells because they can develop into a helpful tool to grow heart cells. Furthermore, the experiment’s goal is that “[the] lab created cells could be used to grow new heart tissue or repair heart muscle damage” (Ogilvie, 1). If people continue to put their hope in the benefits of stem cell research, then eventually it will be able to not only just grow heart cells but the tissue itself.

In order to obtain relevant information to support this topic, databases including PubMed, Medline, UpToDate, and Google Scholar were used. Terms used in each search included and combined the following: mesenchymal stem cell, cardiac stem cells, HF, heart repair, ejection fraction, cardiac output, cardiac index, mortality.

After patients have gone through heart disease, like any other injury, therapeutic steps must be taken. Therapy and rehabilitation of damaged hearts has been improved through the utilization of stem cells. Therapy includes strengthening of the damaged area as to prevent the incident from reoccurring and getting the patient back to their normal life. Scientists, again, used rats that had had induced myocardial infarctions. This

Embryonic stem cells are repairing damaged tissues in the body. Scientists have find a way to replace damage hearts. The United States heart failure affects more than 400,000 people a year. With the use of stem cells can be used to reconstruct the heart

A PBS special that discusses the history and innovation of artificial hearts, entitled “Electric Heart,” demonstrates the use of animal research in biomechanics.

Rather than relying on surgical methods meant to replace these tissues, scientists are now beginning focus on new methods that actually allow the heart to repair and replace damaged tissue. In the June 29, 2006 edition of the journal entitled, Nature, Dr. Kathryn Ivey of the Gladstone Institute of Cardiovascular Disease explains, “the ability of circulating cells to produce factors that are sufficient to invoke cell survival or repair responses in damaged heart cells” remains a primary objective of research cardiologists. By manipulating synthetic compounds meant to catalyze the regeneration of tissue, the new experimental method of repairing heart muscles may be able to abandon surgical procedures altogether. While the vast majority of attention is being spent on the ability to use stem cell therapy to repair congenital heart defects, some scientists are exploring alternative possibilities in organisms like the

Stem cell research indicates that these cells can help carry out organ transplants, treat various diseases affecting human beings, and aid in repairing and replacing various body tissues. One reason stem cells, especially embryonic stem cells, are so effective at treating diseases is they are free from genetic disorders. This ensures a significantly high success rate at curing the ailments they are used to treat. Stem cell transplants, if widely adopted, could give lasting solutions to many medical problems facing humanity. This will be possible because the embryonic cells, or the reprogrammed somatic cells, if induced to sick people, can help regenerate, repair or replace worn out, damaged, or diseased tissues. This will help get solutions to organ malfunction, through removing affected organs and then inducing stem cells, which then help to regenerate or repair the affected organs

“Through the isolation and manipulation of cells, scientists are finding ways to identify young, regenerating ones that can be used to replace damaged of dead cells in diseased organs. This therapy is similar to the process of organ transplant, only the treatment consists of the transplantation of cells rather than organs. The cells that have shown by far the most promise of supplying diseased organs with healthy cells are called stem cells.” (Chapter Preface)

The stem cell therapy known as Ixmyelocel-T is a phase 2 clinical trial viewed as a potential treatment for class III and IV heart failure. Heart failure, which affects an estimated number of about 5.1 million people in the United States, is a condition in which the heart progressively weakens and isn’t able to pump enough blood to meet the body's needs; causing the heart muscles to weaken and degenerate because of the lack of blood flow. Class III and IV heart failure do not have treatment options other than a heart transplant and so, this clinical trial is viewed as an alternative treatment for heart failure.

The term “ventricular remodelling” includes a complex of anatomic, functional, cellular, and molecular changes in the myocardium in response to the injury Markers of cardiac remodelling are hemodynamic and echocardiographic changes which correlate with cardiac impairment (Cohn et al., 2000; Kubanek et al., 2013). In HF animal models, the process of left ventricular (LV) remodelling begins rapidly, and continues to progress; LV are a greater cardiac chamber dilation, a greater increase in systolic and diastolic volume index and a progressive decline in the ejection fraction (EF) (Eaton et al., 1981; Korup et al., 1997; Cohn et al., 2000; Kubanek et al., 2013).

According to some researches, stem cell-based therapies have the potential to dramatically transform the treatment and prognosis of HF by achieving what would have been unthinkable only a few years ago. They also asserted that for the first time since cardiac transplantation, a therapy is being developed to eliminate the underlying cause of HF, not just to achieve damage control. In 2001, the first study of bone marrow cells in experimental myocardial infarction (MI) was published,9 within a year, this therapy had been applied in patients.10 In the setting of HF, it took only three years from the first use of stem cells (skeletal myoblasts) in an animal model to the first use of these cells in

On January, 2008, Doris Taylor, director, Center for Cardiovascular Repair, University of Minnesota used the core procedure of decellularisation to let hearts from animal cadavers beat by infusing live cells. The research