Cell Viaability Lab Report

According to the oral cancer foundation, “Chemotherapy is the use of chemicals to destroy cancer cells. Chemotherapy works by interfering with the cancer cell's ability to grow. It is one of the three main methods utilized to treat cancer.” With cancer being a potentially fatal disease it is important to diagnose it as soon as possible. the oncologist have to study the chemicals used so that they can determine what chemicals will treat the cancer or disease the best and what chemicals can be mixed together without any major side effects. The drugs can be divided into groups based on how they work, their chemical structure, and their relationship to other drugs.

Chemotherapy is the use of one or more anticancer (cytotoxic) drugs to damage cancer cells so they can’t grow and reproduce. Chemotherapy is most effective on cells that are actively growing and dividing. Because many cancer cells tend to grow and divide quickly, they are sensitive to the effects of chemotherapy.

In America today finding a cure for cancer is a huge priority with clinical trails being a big part in these goal there are some newly developed drugs and technology that are making a huge impact in the cancer world.

Cure – The desired outcome for all patients, but one that is not always achievable.

Chemotherapy in cancer is an important aspect of drug treatment. Alkylating agents which work by impairing cell division and antimetabolites which interfere with enzymes while blocking vital cell processes are used to treat malignant cells but have serious setbacks.

Based on the case study, Mr. Fox, diagnosed with Hodgkin’s disease, was prescribed with different chemotherapeutic drugs. The regimen combination is important because each drug acts “at different places in the cell cycle process of cell growth and

. The minimally invasive and localised treatment utilize components that are non-toxic when used independently. The effects of PDT include damage to tumour vasculature, direct killing of tumour cells via cytotoxic activity, and inducing an antitumor reaction.

Cancer is a disease that has taken the world by storm. When chromosomes begin to mutate, they build up and cause a mass to form in or on a person’s body, which eventually turns into cancer. Because of the severity and recurrence of this potentially fatal disease, scientists have been dedicating their time in hopes of finding a cure. Of all the forms of treatment patients undergo, the most familiar of them would be chemotherapy. Even though it is most commonly used, there has been debate on whether chemotherapy is the best treatment option. When treating cancer, there are more options to improve the chance of living and quality of life that does not include chemotherapy.

The most important utility of tumor heterogeneity quantification lies in its implications for determining and defining therapeutic outcome in cancer. Onco-therapeutic interventions fail for two general reasons: 1) cellular resistance, in which the cell is unresponsive to a treatment because of its intrinsic properties (such as activation of xenobiotic

In this paper, possible courses of treatment for cancer will be defined and discussed. Due to there being so many types of treatments, it can be difficult to determine which works better Through examination of case studies in lung cancer trials, it is evident which treatments are the most effective. One also has to take into account the issue of every individual being different, having different genetics, and different types of cancer. In conclusion, the most effective treatment, is in fact, the one that works best for the patient.

Cancer chemotherapy is one of the most effective and successful treatments for curing cancer. The main advantage of this therapy is that it destroys the cancer cells. It is given in several doses in the form of pills or injections. Sadly, chemotherapy is followed by certain side effects, which, in certain cases, may not disappear after the treatment is over. Therefore, before undergoing chemotherapy, it is important that the patients should have strong will-power and determination to undergo this therapy. The main aim of this type of treatment is to cure and control cancer as well as ease the cancer symptoms at the earliest.

Cancer chemotherapy inhibits cancer via several potential mechanisms for example induces cell death, trap cell growth at certain stage, or induce cell differentiation.

Besides the vehicle control group, a CDK cell cycle inhibitor and a selective Bax activator will be used as positive controls based on previous literature. MTT assay and CyQUANT direct cell proliferation assay (Thermo Fisher Scientific) will be conducted to measure the cell viability, whereas Scratch assay and Boyden chamber assay will be conducted to measure the cell migration. The most effective coumarin derivative will be selected in terms of inhibiting the cell proliferation and migration, and potencies from in vitro study will be used to determine the dose and the concentration for in vivo study and specific aim 2 respectively. The orthotopic patient-derived xenografted mice model will be used for in vivo study. Tumor volume will be measured using magnetic resonance imaging (MRI) every week for 2 months. After that, mice will be sacrificed and organs (breast, brain, lungs, and stomach) will be tested for any possible metastasis.

Cancer, the uncontrolled, rapid and pathological proliferation of abnormal cells is the growing public problem whose estimated worldwide new incidence is about 6 million cases per year. It is one of the most dreaded diseases of the 20th century and spreading further with continuous and increasing incidence in 21st century. This is the second leading cause of human death after cardiovascular diseases in developing as well as advanced countries. Although there are many therapeutic strategies including chemotherapy and radiotherapy, high systemic toxicity and drug resistance limit the successful outcomes in most cases

There are basically two important components present in the cancer that are the defense against the immune attack is (1) Myeloid derived suppressor cells and (2) Regulatory T cells. When these two cells are present, it affects the health of the person suffering from cancer. They mainly suppress the activity of immune system towards Cancerous cells. In a very short course of the drug, when given regularly to the patients will help to decrease these two types of cells from place where the tumor is present in the body. This drug will also increase the percentage of the tumor specific CD8+T cell in circulation of the cancer patient blood. It will also promote the activation on CD8+T cell in the tumor site. This drug could also help to reduce the tumor’s ability for defending itself from the immune system of patient. And for the best results the drug is also combined with the tumor vaccines which will cause more potent immune response to the body. The next effect is that it can produce is that it will reduce recurrent rates of cancer in the