Cellular Respiration Lab

1. During exercise: TV will increase. 2. During exercise: IRV will decrease. 3. During exercise: ERV will increase. 4. During exercise: VC will not change. 5. During exercise: TLC will not change.

This experiment tested the hypothesis that there is no difference between cellular respiration before exercising and cellular respiration after exercising in terms of rate of cellular respiration.

Our experiment was performed in the Science and Technology build on the USCB Bluffton Campus on October 6th from 4:30 to 5:30 p.m. In order to construct the study for this experiment, we divided the actual experiment work in order to be able to complete the study for all sixteen (16) subjects within the given time period available to accommodate the test subjects. The experiments was conducted in the hallway, during the process we tested three participants at a time. We constructed an informational form regarding gender, age, weight amount of exercise per week. The supplies needed were approximately 25 test participants for the class, informational form, a blood pressure reader, timer, 15 jumping jacks, three chairs, paper and pencil to record

This results the heart rate to increase because “muscles use more oxygen and glucose from the blood with increased movement”(Heart rate increases, 3). When running the mile, muscles are used constantly, and require energy. The time you get when running is what the experiment is trying to measure.

To find the specific cellular respiration rate, a respirometer was used. A respirometer is a tube that contains the organism, KOH, and barrier between the KOH and the organism. The bottom of the respirometer has weights to keep the bottom of the respirometer submerged in water. A pipet blocks the opening on top of the respirometer. When the organism consumes oxygen, the pressure of the gases inside the respirometer decreases. As the oxygen, water enters the vacant area in the pipet. The KOH combines with the carbon dioxide produced by the organism to create a solid called k2co3. The k2co3 allows the pressure difference of the reduced oxygen to match the rate as the production of carbon dioxide.

The experiment is start by setting up the equipment and use the result for normal breathing as baseline data. After the subject who have no relevant medication condition signed the consent form, the subject should relax and sitting in a chair such that the subject’s mouth is approximately level with the mouthpiece attached to the flow head. The oxygen saturation of haemoglobin is measure by an oximeter that placed on the fingertip of the index finger. The fingernail and connection should both face upward. During data collection, the subject should not move their hands and rest their hands in their lap with palm turned upward. After that, connect the Powerlab to the computer for collecting data. Once the Powerlab was set, type “Normal Breathing”

Also it is important to consider the spectrometers used in this lab, which had capped out above an initial absorbance of 2 and higher in the lab, which skewed all future readings since it was impossible to record from that value, which led to inaccurate readouts for the absorbance values. A major source of error was that a different volumetric pipette was used to measure the initial absorbance and in Trial 1a than the micropipette used in the other four trials. Though both volumetric pipettes were 1 mL, the possible miniscule differences between the two glassware could have created a significant difference in the tiny volumes. The experiment could have been improved by use of a UV/Vis rather than a Spec, which could improve the precision and accuracy of the measured absorbances and thus decrease the uncertainty. Additionally, measuring the absorbances for a longer duration of time so that a significant deviation from linearity could be observed could decrease the uncertainty in the reaction order with respect to Bromophenol blue. The best alternative could involve measuring manipulated concentrations directly against time instead of absorbance since the values would be in our control instead of in the machine’s control, which tended to show inevitable

The experiment began by taking the height and weight of all of the participants. Those taking part performed two trials on separate days. For the first trial, participants consumed water at leisure throughout the day, then at 3:00 pm, preceded to the gym to begin the experiment. Before exercising, the first participant placed the Suunto brand heart monitor under their shirt on their breastbone and fastening the display watch on their right wrist. The participant’s heart rate was recorded before beginning the exercise. The wattage of the Precor RBK 835 Recumbent exercise bike was then set to 80 watts and the participant rides the stationary bike at an intensity that maintains the wattage range between 80 and 90 watts. After five minutes of riding,

The results show that Partner 1 had a resting heart rate of 80 bpm before any exercise. Partner 2 had a resting heart rate of 82 bpm before any exercise was performed. After the first round of going up and down the stairs, Partner 1’s heart rate was 100 bpm and Partner 2’s heart rate was 120. After the second round of the same exercise Partner 1’s heart rate was 140 bpm and Partner 2’s heart rate was 180 bpm. Furthermore, after the last round Partner 1 had a heart rate of 180 and Partner 2 had a heart rate of

Introduction- background information(significance of addressing this problem towards biochem)/why are they doing the experiment/what question are they trying to answer (broad/hypothesis)- at least one page

In this experiment, an apparatus called a respirometer was used. A respirometer is a tool used to observe exactly how much oxygen was consumed by the peas and the beads. The purpose of this lab was to measure the rate of cellular respiration. There are three ways to measure the rate of cellular respiration. These three ways are by measuring

much oxygen is used while exercising than during resting. The inflow of the oxygenated blood

My graphs, shows the pulse rate increases, though out the exercise. Pulse rate increased to 124 beats in 5 seconds than resting pulse rate, after the 10 seconds exercise, increased to 129.5 beats, after 15 seconds increased to 133, after 25 seconds of the exercise was over 136. The increasing intensity levels of exercise causes the pulse rate to increase regularly

When an individual takes part in long periods of intense exercise, their performance decreases and they can become tired over time. However, there is still so much research understanding the cause of this. The experiment conducted by Hettinga et al discusses the idea that an individuals output can be influenced by hot temperatures leading to fatigue and exhaustion. This experiment also explored the idea that an individuals increase in skin blood flow could also explain individuals decrease in their performance [2]. Many others have explored this idea in cycling and the effects that cycling intensity can have on an individual’s performance. One study described and explored the idea that high skin temperature can cause a decrease in exercise intensity. The idea stated that an individuals power out put is linked to an individual’s core body temperature. If an individual’s power out put decreases, their body temperature rises [2].

This experiment is important for biologists and most importantly sports doctors because they can monitor changes and performance of athletes. Many athletes are constantly exercising and under vigorous activity. When studying the physiological changes of the body