Abbreviations:
GRAS: Generally Regarded As Safe
FBA: Fructose Bisphosphate Aldolase
FBP: Fructose Bisphosphate
GAP: Glyceraldehyde 3-Phosphate
DHAP: Dihydroxyacetone Phosphate
MSA: Multiple Sequence Alignment
Introduction
Lactococcus lactis is a Gram positive bacterium commonly used to produce buttermilk and cheese. In most cases they are known to produce Lactic acid. Traditionally regarded as a GRAS microbe, and with its ability to produce lactic acid, it is one of the most important organisms used in the dairy industry, used extensively in the early stages of production of several kinds of cheeses(Siezen, Bayjanov et al. 2010). A good understanding of the genetic and enzymatic information of the cycles that drive the production of lactic acid in these microbes is essential for the dairy industry.
The purpose of this report is to discuss about Fructose 1,6-Bisphosphate Aldolase Class II in L. lactis. However, studies on several other bacteria like E. coli, Streptococcus sp. and other Lactococcus sp. will also be used as reference containing similar versions of this enzyme taking part in their cycles.
Name EC Number Accession Number Protein Length Gene Length Organism
Fructose-bisphosphate Aldolase 4.1.2.13 CDG05391 298aa 897bp Lactococcus lactis
Table 1: Details on FBA. Source:http://www.ncbi.nlm.nih.gov/protein/CDG05391.1
The Enzyme is also known as Fructose 1,6-bisphosphate Aldolase, FBA, Fda, FBA, or simply Aldolase.
FBA catalyses the reversible reaction in Glycolysis
In this case, digestion is left to bacteria living within the digestive tract (Health and Science Pipeline Initiative, n.d.). An important example of this bacteria is Lactobacillus Acidophilus, commonly referred to as L. acidophilus (Robertson, 2017). It is of the Gram-positive species and the Lactobacillus genus (Hammes, n.d.). It can be located in large amounts in the small intestine, where it assists in the fermentation of indigestible carbohydrates and the production of lactic acid (Williams, n.d.). Another microbial bacteria that produces lactic acid is Bifidobacterium longum.
1. Whole milk : Packed with calcium, protein, and other nutrients, whole milk has many beneficial properties. Individuals who are looking to improve their brains can discover that it has alpha-lactalbumin that increases plasma tryptophan availability and boosts day time alertness and brain measures of attention. Furthermore, it aids in sleep which can help you function better in the morning.
Aldolase B acts on Fructose-1-phosphate which is majorly produced through phosphorylation of dieatary fructose in the liver. Other sources of the substrate in small amounts include the proximal epithelium of the renal tubule ad the intestinal mucosa. Fructose-1-phosphate is formed through phosphorylation of fructose by frucokinase enzyme at carbon number 1. Production of fructose-1-phosphate is the rate limiting step in fructose metabolism. An accumulation of the substrate can cause liver toxicity and induce cell death which can eventually lead to hepatic failure or liver disease.
The fermentation that occurs during the making of good silage focuses on the conversion of carbohydrates from the plants anaerobically converted into lactic acid. This is an incredibly simple process brought on by the homolactic Lactobacillus bacteria as the carbohydrates such as glucose as converted into 2 molecules of pyruvate by glycolysis, which is then converted into 2 molecules of lactic acid; creating ATP and involving both the reduction of NAD+ for use in glycolysis, but then also the oxidation of NADH to regenerate more NAD+ (Morton J S, 1980). However, secondary fermentation can be taken by the Clostridia bacteria if the pH does not increase fast enough, which convert the produced lactic acid into butyric acid, increasing the pH but
Aldolase B is classified as a Lyase, or an enzyme that catalyzes the breaking of various bonds means other than hydrolysis and oxidation. Lyase often forms a double bond or a new ring structure. Fructose Intolerance is caused by mutations in the aldolase B gene which is expressed in the liver (Malay, Allen, and Tolan). Its molecular compound is shown in Figure 2 and has two active site residues, Lysine and Glutamate, as well as several charged residues. The functional assignment is complex because of the proximity of the many charged amino acids found at the active site. Figure 3 shows where the substitution of Proline for Alanine occur at position 149 (Malay, Allen, and Tolan). The A149P substitution is known as AP-aldolase (Malay, Procious, and
During my experiment I noticed multiple things with all of my liquids. Apple juice took quite a while to start to boil, but once it started to boil it boiled very rapidly. When I boiled milk I saw a couple things, when I started to boil the milk it boiled and heated up very quickly. Also once my milk boiled it started to create big bubbles so I dumped it out and when I went to rinse it out it stuck to the bottom of my pot. When I boiled my water it took a little while to heat up like my apple juice but when it started to boil it went quicker than my other liquids.
Conventional wisdom has it that human breast milk provides ideal nutrition for newborns and is needed for healthy infant growth and development. In addition, human milk also acts as a conduit for omnifarious immune bioactive factors that facilitate some of the numerous benefits of breastfeeding. Human breast milk consists of rich concentrations of secretory IgA, which works to protect neonates from detrimental viruses and bacteria. Epidermal growth factor (EGF) as well as other growth factors like TGFβ1, and TGFβ2, TNF-receptor I (TNF-RI), and cytokines such as TNFα, IL-6, IL-10, IL-13, and IL-8 are found in colostrum, a form of milk produced by the mammary glands of mammals in late pregnancy. Lactoferrin, proteins, lysozyme, and bifidus factors are also exhibited in the components of breastmilk. Human milk contains a wide array of proteins that provide biologic activities ranging from antimicrobial effects to immunostimulatory functions in neonates. Proteins like lactoferrin, secretory IgA, kappa-casein, lactoperoxidase, haptocorrin, lactadherin and peptides formed from human milk proteins during digestion play a role in inhibiting the
What gene is related to hereditary fructose intolerance: The ALDOB gene which is usually found in the liver. This enzyme called adolese
The bacteria, Lactobacillus Casei, is a “good” bacteria. That means that this bacteria is helpful and does not harm society or the human body. This bacteria is rod shaped and is generally found in the human intestines or mouth as well as fermented plant products. However, this bacteria is also found in dairy products as well as intestinal tracts in animals.This bacteria is also non sporing, non motile, anaerobic, and does not contain cytochromes. The Lactobacillus Casei produces lactic acids that are helpful, For example, the lactic acids help create diary products such as cheeses and yogurts. The Lactobacillus Casei also has digestive benefits, allergy benefits, improves immunity, and improves cholesterol levels.
2. Introduction: Each student was given unknown bacteria and was instructed to perform a variety of experimental tests that would help to identify their bacteria. During the process of identification, the unknown bacteria was added to many different testing medias using aseptic technique. They are as follows: lactose fermentation on eosin methylene blue (EMB), TSI (Triple Sugar Iron agar), Phenol red sucrose, the SIM test, H2S by SIM, IMViC (indole, motility, voges-proskauer, and citrate), Urease (urea broth), PDase (Phenylalanine Deaminase), Lysine Decarboxylase, and Ornithine Decarboxylase. Colonial morphology on EMB was used to
Introduction: Through the conduction of numerous experiments, the identity of two bacterial isolates was determined. The tested specimen was an unknown sample of a mixed culture of two different species of bacteria. The first step that was taken was obtaining a pure culture of each species of bacteria by isolating one species from the other. Once isolation was complete, the isolated cultures were tested using procedures that had been performed during previous lab sessions. A gram stain was performed on the two isolates. The isolate which had tested gram negative was then tested for the presence of cytochrome C and lactose fermentation. For the gram positive isolate, cell shape was determined and a catalase test was performed.
Currently, there is a lot of research exploring the development of food-grade microorganisms to control the production of desirable enzymes and proteins. The point of this article is the try to find an inducible expression system, similar to the one already discovered in E. coli. In this article, L. lactis is explored as a possible inducible expression system. L. latis is a lactic acid used in the dairy industry and other food fermentations. It is studied in this experience because it has the potential to be a safe production host for the development of food-grade microorganisms. Safe genetic tools (acceptable for food production) have already been developed to aid in the potential production of food-grade
Milk curds are a dairy product that is formed by coagulation, also known as clotting. Curds can be formed when rennet or any acidic liquid is added to milk. Lemon juice and vinegar are two common acidic liquids that provide benefits when creating the formation of milk curds. Increased acids, such as vinegar or lemon juice, force proteins contained in milk to group together and create solid masses, which are identified as curds. Any remaining liquid after this process is whey protein. Cheese curds contain proteins and fat from milk. They are produced by a formation called coagulation of casein. Casein is unique in the way that it is not coagulated by heat. Milk becomes sour when lactic acid bacteria streptococci and
In this experiment, milk is separated into its different chemical components and qualitative tests are used to determine what the components are composed of and to determine the chemical structures.
Several lactic acid producing miroorganisms such as lactic acid bacteria, fungi , yeast and microalgae were analyzed for the fermenetation process of the lactic acid production. Since the fermentation step is the producing step of the whole it is very essential that the chosen strain is feasible, economically compeptitve and yields efficent amount of desired product which is lactic acid.There were several criteria that were considered during the elimination process for the microorganims such as the ph tolerability which is correlated with the acid tolerancy, thermal resistivity in order to determine the safety measures of the process, the type of feremntative process that is implamented by the organism and the efficency were among these criteria.First