Chemistry 100 Labreport Essay

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Determination of Vanillin in Imitation Vanilla Essence
Chloe Nolan 14832679

4-hydroxy-3-methoxybenzaldehyde or as it is better known, vanillin is most commonly used to create imitation vanilla essence (Southam, 2013).

Figure 1: vanillin structure

Vanillin was first synthesised from eugenol from 1874 – 2001, from where it was later made from lignin- containing sulphite liquor which is a by-product of wood pulp processing in paper pulp manufacturing, most recently it is more common to find vanillin made from guaiacol and glyoxlic acid (3D chem, 2013). Vanillin is most commonly found in food flavour formulations, perfumes and fragrances and has been used in drugs used by those who suffer from Parkinson’s disease
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Diluted essence (10ml, 100 times diluted) was pipetted into a 100ml separating funnel. 20ml of dichloromethane was added to the diluted essence. The funnel was then shaken vigorously for 2 minutes with gas being release occasionally. The organic phase (lower layer) was released into a 100ml beaker and another 20 ml of dichloromethane was added to the funnel and shaken vigorously for another 2 minutes. The organic phase (lower layer) was again released into the 100 ml beaker. The contents of the funnel were disposed of. The organic phase was then added back into the separating funnel with 40ml of 0.1M sodium hydroxide solution and shaken well for 2 minutes. The organic phase was released and disposed of and the aqueous phase was kept and transferred to a 250ml volumetric flask and the volume was made up with 0.1M sodium hydroxide. Standards were then made using the 50mg/L standard provided and mixing with 0.1M sodium hydroxide to create standards with 1,2,3,4,5 mg/L concentrations. Each solution was run through the UV – Visible spectrometer to find the absorbance and then the concentration.

Results and Discussion [Vanillin] mg/L | Absorbance | 1.00 | 0.195 | 2.00 | 0.380 | 3.00 | 0.540 | 4.00 | 0.750 | 5.00 | 0.940 | Your vanilla essence extract | 0.430 | Solution for 100% extraction efficiency | 0.450 |

Table 2: UV-Visible Spectrometry Results
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