Chlorophyll And The Concentration Of Chlorophyll

There are two main types of chlorophyll, chlorophyll a which absorbs wavelengths of 430nm (blue) and 662 (red) and is the main photosynthetic pigment, and chlorophyll b, which doesn’t directly participate in the photosynthetic process, but is capable of donating its energy to chlorophyll a

Photosynthesis is the conversion of light energy to chemical energy into sugars. It is the process in plants that uses carbon dioxide, water, and sunlight from its surroundings and releases oxygen as a byproduct (6H2O+6CO2+light energy -> C6H12O6+6O2). Photosynthesis is required for plants because they are autotrophs, organisms that make their own food. Plants require a specific environment that is ideal to them to be able to carry out the process. Environmental conditions can either increase or decrease the rate of photosynthesis. Things like colors of light, pH, and temperature can all affect the rate of photosynthesis in plants.

The effects of light intensity and light wavelength on photosynthesis was observed in two different experiments and closer study of different pigments in spinach was observed. For the experiment where the effects of light was observed, it was found that a light intensity of 30 cm, wavelengths of blue and red lights show the greatest photosynthetic activity. The different pigments present is spinach were also observed and it was noted that though chlorophyll is the most abundant in plants, there are other pigments present as well.

Abstract: Photosynthesis, the conversion of inorganic carbon into organic glucose molecules using light energy, is one of the most biologically important processes on Earth. It is imperative to study how the rapidly increasing carbon dioxide concentrations in the atmosphere since the Industrial Revolution may affect photosynthesis of photoautotrophs. In this experiment, a look is taken at the question: does inorganic carbon availability affect photosynthetic activity. This experiment uses bicarbonate as the inorganic carbon source, and analyzes how varying concentrations of bicarbonate may affect the photosynthetic activity of the South American aquatic plant Egeria densa (also known as Elodea densa) by measuring its O2 production in distilled water and 0.1%, 0.4%, 0.6%, 0.8%, and 1.0% sodium bicarbonate solutions. T-tests between the control (distilled water) and each bicarbonate treatment are conducted using the online program GraphPad. All tests results in a p-value greater than 0.05 and a calculated t-value greater than the critical t-value, thus rejecting the null hypothesis, indicating that inorganic

The purpose of this experiment was to investigate the effects of light intensity on the rate of photosynthesis in a Moneywort plant. By observing the plant in distilled water mixed with sodium bicarbonate, different light bulbs were targeted onto the plant. The measurement of the amount of bubbles present on the plant during the trial of the experiment enabled us to identify the comparisons between the activity of the light and the process of photosynthesis.

Sargassum is used by larvae to travel to other locations and this allows the ocean to be more diverse. They also produce oxygen since they are photosynthetic. Algae is not a Linnaean classification but they are in kingdom Chromista and Plantae. Algae has three grouping of brown, red, and green algae. Brown algae contain chlorophyll an and c and are in kingdom Chromista. Red algae contain a pigment called phycobilins and only contain chlorophyll and are in kingdom Plantae. They are used as a food additive in ice cream and used in sushi. In their blooms they become known as the red tide and will secrete nerve toxins in the water. Green algae contain chlorophyll a and b and are in kingdom Plantae. Some of the common are dead mans finger, sea lettuce, and mermaids

Abstract: The purpose of this lab is to separate and identify pigments and other molecules within plant cells by a process called chromatography. We will also be measuring the rate of photosynthesis in isolated chloroplasts. Beta carotene, the most abundant carotene in plants, is carried along near the solvent front because it is very soluble in the solvent being used and because it forms no hydrogen bonds with cellulose. Xanthophyll is found further from the solvent font because it is less soluble in the solvent and has been slowed down by hydrogen bonding to the cellulose. Chlorophylls contain oxygen and nitrogen and are bound more tightly to the paper than the other pigments.

Kelp forests are seen as the ultimate ecological engineer in coastal areas. They exist in marine environments spanning in southern California, Aleutian Islands, and the western north Atlantic. Light, latitude, and water temperature and nutrients all play a role in facilitating the growth of kelp forests all around the world, (Bolton). Kelp specifically dominates the shallow rocky coasts of the world‘s cold water marine habitats. Although they look as a free-growing plant, they are in fact primarily of brown algae. These structures provide food and shelter to a huge mix of biota, including crabs, sea urchins, mollusks, and other marine organisms (Steneck). The fronds, or large leaves of the kelp are the distinctive feature that can represent where a specific kelp may be found. The sizes of these organisms can range from the smallest found near California and Alaska at 5m to giant kelp up to 45m in length found on the west coasts of North and South America (Hamilton).

The experiment took place in a laboratory setting, and the first step was obtaining sixty individual Daphnia magna (that were neither adults nor tiny offspring) from a large tank in the lab. These individuals were equally divided into three groups; low density, medium density, and high density. The twenty Daphnia assigned to the low density group were split into four groups of five and pipetted into one of four tubes filled with 10mL of Chlamydomonas algae. The twenty Daphnia assigned to the medium density group were split into two groups of ten and placed into one of two tubes also filled up to 10mL with Chlamydomonas. The final twenty Daphnia were all placed into a single tube filled with 10mL of the algae. In order to avoid suffocation-related

The initial experiment was a success. As our treatment group spent more and more time under the lights, the absorbance rate continues to decrease toward zero. Once our 30 minutes were up, the absorbance rate in each tube was significantly lower than at the start of our experiment. In contrast the two control groups did significantly lower the absorbance. Each control lacked one of the vital aspects of photosynthesis, one being light, and the other being chloroplast. Neither of the control groups (Control 1 or 2) showed any signs of photosynthesis. Control 1 was exposed to light, but contained no photosynthetic organelles thus the absorbance throughout the 30 minutes varied minimally, mostly staying stagnant. Control two which contained chloroplast but was not exposed to any light failed to lower the absorbance at all and in fact increased the absorbance over the 30 minutes. However, the treatment group contained both and ultimately performed photosynthesis as we expect therefore, confirming our assumption that chloroplast were the organelles required for photosynthesis in plants and that light is required to perform said photosynthesis. The treatment group, containing both the chloroplast and being exposed to light provided evidence that photosynthesis was taking place as the absorbance lowered at each 10-minute interval. Having a less absorbance would be desired because as DCIP became reduced we would expect the solution to become more and more clear, thus less

The purpose of this experiment is to understand the effects of nutrient enrichment and eutrophication, using samples of water from Rio Salado and Encanto Park. The samples will contain different concentration levels of nitrogen, phosphorous and nitrogen and phosphorous combined and the impact it has on algae growth. The results recorded showed that the nitrogen concentration levels had a little change, phosphorous levels had a higher change and phosphorous and nitrogen combined had a significantly higher change, resulting in higher algae growth. The results showed that phosphorous indeed is a limiting nutrient in algae growth, but to achieve the highest growth rate, both nitrogen and phosphorous need to be combined.

During the separation of the pigments by chromatography paper, chlorophyll b traveled the shortest distance, chlorophyll a went above it, and the highest went beta carotene. This

The purpose of this experiment was to take spinach leaves and extract the chlorophyll and carotenoid pigments by using acetone as the solvent. The chlorophyll and carotenoid pigments were extracted by using column chromography and alumina was used as the solvent. Solvents of different polarities were used, starting with the least polar, to extract the certain components from the leaves. They were then analyzed by using thin- layer chromatography.

For lab 12, it is hypothesized that chlorophylls a and b are present in a plant leaf and contribute to the starch production in photosynthesis. Also, products of photosynthesis will be present in leaf tissue exposed to red and blue light wavelengths for several days, but a decreased presence in leaf tissue exposed to green and black light wavelengths. In lab 13, it is expected that since chlorophylls a and b are more polar and smaller molecules than the anthyocyanins and carotenoids, they will travel higher up the chromatography paper than the other pigments.

Phytoplankton are the basis of nearly all the Ocean’s ecosystems, and require a wide range of inorganic nutrients in order to grow; notably Carbon, Nitrogen and Phosphorous. They take up these nutrient elements according to the Redfield Ratio of C : N : P = 106 : 16 : 1. But phytoplankton also require less obvious nutrients such as calcium, magnesium and potassium. These are often in plentiful supply in the worlds Oceans, and other factors such as the micronutrient Iron can be the limiting Phytoplankton growth factor. Iron is not required by Phytoplankton in large amounts, but the Oceans are deficient of this micronutrient, and without it Phytoplankton cannot grow and reproduce as readily. This is because Iron is required in the biosynthesis of the pigment chlorophyll, required by Phytoplankton in order to photosynthesise. With a deficiency in Iron, Phytoplankton can suffer from a lack of chlorophyll known as chlorosis. The protein ferredoxin contains Iron and is substituted for flavodoxin as an iron-stress response (when Iron levels are low). It can be shown that Phytoplankton are Iron deficient by measuring how much of this electron transfer protein flavodoxin is present.