Chromatography Is An Inexpensive Method For Analysing Some Types Of Chemical Mixtures And The Separation Of Chemicals

1039 WordsMay 14, 20155 Pages
Paper Chromatography: Paper chromatography is an inexpensive method for analysing some types of chemical mixtures and the separation of chemicals or substances. It requires polar chromatography paper - the stationary phase, and a solvent - the mobile phase. It can be achieved by applying evenly separated marks of the desired mixture onto the paper (filter paper is one of the best choices, although paper towels and even newspaper can also be used) and immersing the bottom of the paper into the solvent without contacting the mixture mark. As the solvent slowly travels up the paper, the different components of the mixtures travel at different rates determined by their individual polarity. A mixture that resulted in a higher place up the paper…show more content…
This layer of adsorbent is known as the stationary phase. After the desired sample has been applied on the plate, it’s bottom is dipped in a solvent mixture. The solvent or solvent mixture (known as the mobile phase) is drawn up the plate via capillary action. Because different analytes ascend the TLC plate at different rates, separation is achieved. Similar to that of Paper Chromatography, the mark or analyte that had travelled the least had a stronger tendency or attraction to the plate than an analyte (non-polar) that travelled further due to it’s polar nature and that of the plate. Application/Use: This Layer Chromatography applications are also involved in forensic studies where it is used to examine fibre dye composition. It is also applied in the examination of pesticides and/or insecticides in food and the isolation or separation of biochemical metabolites or constituent from its body fluids, blood plasma, serum, urine etc. Column Chromatography: Column chromatography in chemistry is a method used to purify individual chemical compounds from mixtures of compounds. In thin layer chromatography, the stationary phase is a thin layer of silica gel or alumina on a glass, metal or plastic plate. Column chromatography works on a much larger scale by packing the same materials into a vertical glass column. The desired mixture is added to
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