Chromatography Lab Report

When calcium chloride, sodium bicarbonate, and phenol red are put into a bag and mixed up the type of reaction that occurs is a chemical reaction for the following reasons. Things that indicate that a chemical reaction has occurred are color change, formation of precipitate, formation of gas, and energy transfer in which three of them took place in the reaction of calcium chloride, sodium bicarbonate, and phenol red. The three indicators in this experiment were: a color change, formation of gas, and an energy transfer

The mole is a convenient unit for analyzing chemical reactions. Avogadro’s number is equal to the mole. The mass of a mole of any compound or element is the mass in grams that corresponds to the molecular formula, also known as the atomic mass. In this experiment, you will observe the reaction of iron nails with a solution of copper (II) chloride and determine the number of moles involved in the reaction. You will determine the number of moles of copper produced in the reaction of iron and copper (II) chloride, determine the number of moles of iron used up in the reaction of iron and copper (II) chloride, determine the ratio of moles of iron to moles of copper, and determine the number of atoms and formula units involved in

The investigation is showing how enzymes work inside a mammal's stomach. Rennin is the enzyme found in young mammals and has more effect

Purpose: The purpose of this lab is to further observe four types of chemical reactions. This lab will show four types of distinct chemical reactions and will increase my knowledge on each type.

Purpose: is to determine the unknown bacteria with a variety of biochemical tests. There are many reasons that contribute to why it is so important to test patients for both high and low risks diseases. The most important reason would be to know the identity of microorganism and how it can be treated .This study was performed in microbiology laboratory class by applying the microorganism to the tests that have been performed in the class prior to the identification of the unknown. First, the lab professor handed out a bacteria that was on the unknown streak plate labeled B5 that consisted of an unknown gram positive or gram negative bacteria.

Data were collected in September and November 2017 at the Elk Valley Preserve and Field Station in Banner Elk, NC. The preserve is located at 36°10'17.2"N 81°54'45.9"W, in Avery County in western North Carolina. The area is a mixed deciduous forest at an altitude of 1,127 m above sea level. Data were collected in early fall, during which temperatures average around 10-18 degrees Celsius. Summers in Banner Elk are typically mild, with temperatures averaging 21 degrees Celsius, and winters are cold and snowy, averaging near zero degrees Celsius. During the months of September and November there is an overall average precipitation of 12-25cm.

Patient #1 will need several lab and diagnosis test ran on the current medication list. A CBC and culture & sensitivity test is needed for the Vantin to determine if still needed for bacterial infection. Potassium level along with renal function, bicarb and pH level should be monitored for mineral and electrolyte replacement of potassium Chloride. Furosemide (Lasix), which is a diuretic, is being administered 40mg PO daily. Electrolytes, renal function, hepatic function, glucose levels along with uric acid level should be checked before therapy begins. Also, check for decrease in potassium, sodium, calcium and magnesium. Patient #1 is on several benzodiazepines Librium 25mg Q8h, Ativan 1mg every 1-2hrs orally as needed. And Serax 15mg

In the chemistry world, there are thousands of known chemical reactions that either occur in living systems, in industrial processes, or in chemical laboratories. Few of the types of reactions include Combustion, Synthesis, Decomposition, Precipitation, Single-Replacement, Double-Replacement, Acid/Base, and Redox. It is useful to be familiar with the different types of reactions, because it allows one to know what they are dealing with in a reaction, as well as being able to predict what might happen in additional chemical processes. The first basic type of reaction is Combustion.

Before starting all the biochemical tests, an inoculation procedure must happen. An unknown test tube was handed out which contained two different cultures. The unknown culture was place on a nutrient agar plate using the three-streak inoculation technique for isolation (Lab Manual, Chess 2015). After a few days of growing, the presence of two unknown bacteria had a clear visible of separation. Following the isolation streak technique, the gram-staining experiment was performed. The two bacteria were determined: one was Gram-negative bacteria and the other was Gram-positive bacteria.

Paper chromatography is used to separate mixtures of substances into their components. There are different types of chromatography but they are all based on the same principal. Paper chromatography is an analytical method that is used to separate colored chemicals or substances, especially pigments. They all have a stationary phase and a mobile phase. The moving substance is called the mobile phase while the stationary phase stays put. The mobile phase flows through the stationary phase and carries the components of the mixture with it. The stationary phase is motionless and is the actual medium that performs the separation. Ninhydren reacts with amino acids to give colored compounds and detect the location of the amino acids. This is used because amino acids are colorless. Different components travel at different rates. Each one undergoes adsorption in a slightly different way and spends more or less time in either the solid or the liquid phase. Components of the samples will separate readily according to how strongly they absorb on the stationary phase vs. how readily they dissolve in the mobile phase.

Chromatography is a technique used to separate mixtures of substances into their components^2. It can be used to various activities such as separating different pigment of dyes and inks all the way to analyzing DNA sequences.

Methods: Due to experimental error in previous labs, we did not obtain the DNA of our original taxa. We then chose to use DNA sequences supplied by the TA from the 2016 and 2017 school year. We picked six fungal taxa obtained from differing parts of English Yew Trees, including its fruit, bark, and leaves. Using the DNA sequences given, we copied the sequence into the BLAST system found on blast.ncbi.gov, making sure to add “fungi” into the organism box and to search for somewhat similar sequences. Once the program obtained the results, we scrolled down to the first result that gave a genus and species name, with an “ITS” in the description. Using the information given in the results, we filled in the information on Table 2, including seq

Hagen M, Ashraf M, Kim I, Weintraub N, Tang Y (2018) Effective regeneration of dystrophic muscle using autologous iPSC-derived progenitors with CRISPR-Cas9 mediated precise correction. Medical Hypotheses 110:97-100

In this experiment, the fatty acids of olive oil and sunflower oil were determined and compared to identify the differences. The analysis was conducted using gas chromatography method. Before the analysis, methyl ester was prepared by sodium methoxide method. The fatty acids in lipids are interesterified in the presence of sodium methoxide. The process is known as direct transesterification.

Chromatography Investigation Chromatography is a highly regarded technique used to separate the components of a mixture. It is based on the principle that each component possesses a unique affinity for a stationary phase and a mobile phase. The components that are more inclined to enter the mobile phase will migrate further on the chromatogram and distinguish themselves from the other components. The type of solvent used in chromatography is known to directly affect the separation of the mixture. In this experiment, thin-layer and column chromatography will be utilized to separate the numerous chlorophyll and carotenoid pigments of a spinach extract.