Chromatography Procedure

Background: Paper chromatography is one method for testing the purity of compounds and identifying substances. Paper chromatography is a useful technique because it is relatively quick and requires small quantities of material.

So green apple is green, grapes are purple, cherries are red, oranges are orange, and lemons are yellow. Then I stirred it until it looked good. Then I poured each mixture into their own small pans, make sure they are not glass pans because they will break. Then put the pans into a freezer and wait 4-5 hours or until mixtures are frozen. Then break them into smaller pieces about a nickel's size and put the pieces into the microwave and wait 40 seconds and it should be melted. Now they let the pieces cool and then roll them into balls, and then dipped it into tartaric acid. Let the balls harden.

If you put too much of a sample on your chromatography paper, you could possibly have that color bleed into the color next to it, which would mess up your results. If you put too little of a sample on the paper, your color

Answer: Once the chromatogram has been completed and is ready to be measured and calculated, on the plate that was used to perform the chromatogram you should see where the red and blue have completely separated. The red food coloring dye should be lower on the plate than the blue food coloring dye.

direction from where you can read them. The vials should not be shifted during the experiment and your

The wet, crude product was placed into the 50 mL Erlenmeyer flask. Small amounts of CaCl2 were added to dry the solution. The flask was sealed and the mixture was swirled and left to settle. Once

Prepare standard solution #1, Take 1 ml sub stock solution from the 100 ml beaker and then put into 25 ml volumetric flask with the help of 10ml graduate pipette.

1) What is the surface area of each of your three cells? The agar cylinder had a surface area of 5.06 cm.

Add a few drops of food dye to the water until you like the color you see.

2. 3 real-world uses for paper chromatography include forensic testing, performance enhancing drug testing, and Ebola immunization.

Crystallization was induced in the solution by scratching the inside of the beaker with a glass stir rod. 2. Next, an ice bath was made by partially filling a 400 mL beaker with ice and covering the ice with water. 3. The beaker containing the solution was then placed in the ice bath for approximately 15 minutes.

2. Agitate the beaker carefully for about 30-45 seconds, or until the color comes off of the candy. Be careful to dissolve as little as possible of the white layer (sugar) under the colored layer and do not dissolve any of the chocolate. Immediately decant the colored solution into a clean test tube, trying not to transfer any of the sediment. Repeat with the other colors for which you are responsible.

After being cooled the vial was put into a centrifuge for one minute to separate the liquid and precipitate. The liquid was poured out into a separate test tube to be used in step 3 and the solid for step 2. Step 2 required the use the solid from step 1. 30 drops of deionized water was added to the solid, then heated over a bunsen burner for 3 minutes while being stirred frequently with a stirring rod. A precipitate remained, so the vial was put in the centrifuge for an additional 1 minute.

The following procedure dealt with a chromatogram. The materials needed are: a pencil, safety goggles, scissors, chromatography paper strip, capillary tube, spinach plant pigment extract, test tube, cork stopper, graduated cylinder, chromatography solvent (alternative isopropyl alcohol), metric ruler, stopwatch or clock with a secondhand, hook/fashioned paperclip, paper towels, test tube rack, and mortar and pestle. First we obtained a strip of chromatography paper and cut it so it would fit inside a test tube (with it barely touching the bottom of the tube). Also, when touching the strip, touch the sides only. Then we attached (firmly) the top of the strip to a hook (or fashioned paperclip at bottom of the cork stopper). Make sure it fits in the test tube. Next we used the pencil to draw a faint line across the strip two centimeters from the bottom tip of the strip. We placed the cork and strip in place, and we put a mark on the test tube one centimeter below the top of the stopper.

Figure (3) shows the optical images of the samples, Annealed (AN), Cold rolled (CR), Hot rolled (HR).The designation is An is in section a, CR 20 is in section b, CR 40 is in section c, CR 60 is in section d, HR 60 is in section e, HR 40 is in section f, CR 80 is in section g, HR 80 is in section h, and HR