Classification And Discussion Of Plant Authentication

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CHAPTER-3 RESULTS AND DISCUSSION 3.1 PLANT AUTHENTICATION The Cajanus cajan was collected from Village- Chakneelkanth Dist- Kushinagar, Uttar Pradesh, India, during the month of January 2014. The authentication of the plant was done by Dr. Tariq Husain, Senior Principal Scientist, Plant diversity, Systematic and Herbarium Division, CSIR-National Botanical & Research Institute, Lucknow, Uttar Pradesh and plant specimen has been identified as Cajanus cajan (L.) Huth of the family Fabaceae and a specimen containing voucher number (LWG-037) was submitted for the future reference. 3.2 PREPARATION OF EXTRACT Fresh and healthy Cajanus cajan roots were collected, shade dried, powdered and macerated in 70% v/v ethanol for 72 hrs. The liquid extract was collected and evaporated under reduced pressure by using rotary evaporator (Buchi R-200) at 400C and then freeze-dried in lyophilizer (Labconco, USA) to obtain solid residue. 3.3 GENERAL BEHAVIOR AND ACUTE TOXICITY According to OECD-423 guidelines (Acute toxic class method) and Douli and Sengupta, 2012, none of the doses tested produced any gross apparent effect on general motor activity, muscular weakness, feacal output, feeding behavior etc. during the 14 days of observation. Therefore, ethanolic extract of CC was found safe up to dose of 2000 mg/kg. 3.4 PRELIMINARY PHYTOCHEMICAL SCREENING Preliminary qualitative phytochemical screening of Cajanus cajan root extract in 70% ethanol showed presence of flavonoids, tannins,

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