Clorox Bleach Lab Report

The practical was carried out to investigate the effect of pH on the reaction of the enzyme acid phosphatase.

Three paper towels A, B, and C where tested on absorbency. This experiment was tested twice. Paper towel A was 26.5 by 28 centimeters. Paper towel B was 26.5 by 28 centimeters. Paper towel C was 15 by 28 centimeters. Scissors were obtained and each paper towel was cut to 13.5 by 15 centimeters. The weight of the three paper towels at this size was 0.9 grams dry for paper towel A, paper towel B weighed 0.8 grams dry, and paper towel C weighed 1.2 grams. Three beakers weighing 12.2 grams each were obtained. Each beaker was filled with 100ml of water with the outcome of them weighing 128.1 grams each. The weight of each paper towel dry was deducted from the weight of each paper towel. Paper towel A was left to soak for seven seconds.

The mole is a convenient unit for analyzing chemical reactions. Avogadro’s number is equal to the mole. The mass of a mole of any compound or element is the mass in grams that corresponds to the molecular formula, also known as the atomic mass. In this experiment, you will observe the reaction of iron nails with a solution of copper (II) chloride and determine the number of moles involved in the reaction. You will determine the number of moles of copper produced in the reaction of iron and copper (II) chloride, determine the number of moles of iron used up in the reaction of iron and copper (II) chloride, determine the ratio of moles of iron to moles of copper, and determine the number of atoms and formula units involved in

In the beginning of lab, we were advised to obtain a nutrient agar petri plate, which is used for the cultivation of microbes supporting growth of non-fastidious organisms. Since it contains many nutrients, a wide variety of bacteria and fungi can grow. Taking the plate,

Chemical solutions are different than biological spills because chemicals can do serious damage to living organisms and won’t be completely cleaned in routine waste treatment faculties. Also bleach solution wouldn’t remove acid or base spills they have to be treated different.

In this experiment, there were 6 different test tubes, each containing a different amount of phosphate concentration. Using a balance, 1 g of phosphate in a 400 mL beaker was measured and then inserted into a 100 mL graduated cylinder. 5 mL of ammonium vanadomolybdate (AVM) was added, along with deionized water until the solution reached to 25 mL. Then the solution was poured into one of the six test tubes. Another test tube contained 2 g of phosphate (phosphate was again measured using the balance) and the step described earlier was repeated until five of the six test tubes were filled, each with the same volume of 25 mL. The sixth test tube was filled with only deionized water, with 25 mL as the volume. A spectrophotometer was then used to determine the percent transmittance for each solution. The absorbance was also calculated using the equation A = -log (T). Each given amount of phosphate was converted into moles and then applied the value of .025 L for the volume to calculate the molarity of phosphate in each solution.

The purpose of the sludge lab is to separate many substances that Mr. Beaver gave us from each other and possibly identify them. To do this, Shelstie and Abshir will use different techniques such as filtration, distillation, paper chromatography, and more. It is important to know characteristic properties. Characteristic properties are a substance that has the same result and the amount of the substance doesn’t affect it. For example, in distillation, they would need to figure out what substances boils at this certain degree. For the sludge lab, they will measure boiling point, density, flammability, and more. They need to measure characteristic properties because only one substance has that characteristic property.

Water Quality & Contamination Abstract My report was based on how ground water may be affected by many containments present in our surroundings. I believed that all of the water samples would have been contaminated once mixed with the soil. As I predicted vinegar filtered through the soil and came out fairly clean.

Figure 3 shows the average number of colonies that grew for each bleach solution. When observing the data, there was an increasing trend present up until the 0% bleach solution was tested, where growth decreased. The table shows that the 0.0001% bleach solution had the most bacterial growth on the plate, with an average of 165 colonies, whereas bleach solutions 10%, 1%, and 0.1% had the least growth, with an average of 0 colonies. The 0.01% bleach solution had an average of 49 colonies, the 0.001% bleach solution had an average of 53 colonies, and the 0% bleach solution had an average of 38

The objective for this laboratory is to determine the velocity of alkaline phosphatase and make a graph of rate vs substrate concentration (Michaelis Menten plot). After the wavelength was determined the para-Nitrophenyl phosphate (PNPP, Sigma Aldrich) was obtained to get the absorption spectrum. To obtain a reaction of .5 A/min, a 0.5 of substrate solution was used and 0.015-0.07 ml of enzyme solution as well to bring a total of volume of 1

Results: Three different kinds of bacteria, along with one fungus, were found in the pond samples. Only one bacteria was found in the healthy pond sample this bacteria is morhotype 1 (Table-1). This bacteria was also found in the untreated sample of pond 22 (Table-1). Also in the untreated pond sample was morphotype 2 (Table-1). Morphotype 3 and 4 were found in the pond 22 sample that was treated with colistin sulfate (Table-1). It was later determined that morphotype 3 was a fungus that was caused by an impurity in the colistin sulfate that was used.

Lab 8: Investigation 5 Lab question: How do you separate molecules that are attracted to one another? Procedure: Place a small amount of solvent in separate beakers. Then place the mixture on chromatography paper and put the paper in each beaker. Controls:

There are multiple types of phosphates, including orthophosphates, organically bound phosphates, and finally, metaphosphates, or polyphosphates which come from three different areas. Orthophosphates come from naturally processed materials and are found in sewages. Polyphosphates are used to treat boiling water and help with detergents, but turn into orthophosphates upon reaching the water.The organically formed phosphates are very significant in nature. Organically formed phosphates come from organic pesticides that have phosphates in them. The Team had no results for phosphates. (USGS,

It turns out microbes don't wait for five seconds, or even 1 second to hop on to your food. Even brief contact with a contaminated floor will contaminate food wet or dry! Bacteria will adhere to food almost immediately, but time does matter. The food left on the floor for five seconds acquired a lot of bacteria, but the food left on the floor for 30 seconds picked up a few colonies more. Imagine if you left it for a full minute, or even 10 minutes... the amount of bacteria would be ten times greater! Furthermore, don't think your food is safe because the floor "looks clean" or the food that you dropped on it does. Floors make great homes for bacteria. Also, floors come in contact with our shoes on a regular basis, and the University of Arizona

Testing the Effects of Temperature on the Decomposition of Hydrogen Peroxide with the Enzyme Catalase