Colorimetric Analysis Lab Report Results

The experiments conducted for this lab report focused on water contamination and filtration. Experiment 1 was effects of groundwater contamination. Oil, vinegar, and laundry detergent were added to clean water with no means of filtration. The clean water was found to be contaminated. A filtration system consisting of cheesecloth and 60 ml of soil was created and the contaminated samples were filtered through it. The soil and cheese cloth did not affectively filter the contaminants. Experiment 2 focused on

Luminol is a chemical made up of eight carbon molecules,sevean hydrogen molecules,three nitrogen molecules,and two oxygen molecules. Chemiluminescence the emission of light in a chemical reaction which makes this substance glow a liquid blue when mixed with an oxidizing agent . In the reaction chemiluminescence does not exhibit significant amounts of heat giving off “cold light” via chemical reaction. Chemiluminescence is commonly found in deep oceans where many organisms have glowing organs the same reaction is found in glow sticks and fireflies. Oxidizing agents are a substance that brings out oxidation by reducing the electrons. Oxidation is a loss of electron in a reaction by a molecule,atoms or ion .

The higher her respiratory rate the more liters of oxygen needed to supply for the loss in her lungs. To get the minute ventilation you take the breaths per min times 500mL/breath and that gets your liters of oxygen per min.

Fig. 12 CXL10-/- mice are relatively protected against FFC-induced liver injury and inflammation. WT & CXCL10-/- mice were fed either chow or FFC-diet for 20 weeks. (A) Plasma alanine aminotransferase (ALT) levels were measured. (C) Total RNA was extracted from liver tissue and mRNA expression of surface macrophage marker cluster of differentiation (CD)68 was evaluated by real-time qPCR. (D) Assessment of macrophage infiltration in fixed liver tissue was done by immunohistochemistry using macrophage galactose-specific lectin (Mac-2) antibody. Bar columns represent mean ± S.E.M. *** P < .001, * P < .05 compared to WT chow-fed mice.

The purpose of this lab was to identify unknown bacteria cultures using various differential tests, and my unknown bacteria is #17. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways, each was used in a way to help recognize those specifics and identify the unknown cultures. The differential tests used to identify the unknown cultures were Gram stain, Catalase, Mannitol Salt Agar (MSA), Blood Agar, Novobiocin, Coagulase, and DNAse (Alachi, 2007).

According to the BMI for Erin’s height and weight, she is considered to be under weight. A healthy weight for Erin is between 125 lbs. to 158 lbs. The recommended intake of protein for adults is 0.85 grams per kilogram of healthy body weight per day. Based on Erin’s weight, she should be consuming 43 grams of protein. Erin has been calculating her recommended dietary allowance based on an unhealthy weight and not the midpoint of the healthy weight range, which is 141 lbs. Erin’s recommended daily protein requirement should be 51grams.

Creatinine is a waste product filtered by the kidneys into the blood (serum) and urine. High serum, and/or urine creatinine levels are indicative of kidney dysfunction. A colorimetric assay can be used to determine the creatinine concentration in the urine and serum samples from patients who are suspected to have kidney dysfunction.

The Panasonic Lumix DMC TS30 comes with a resolution of 16.10 Megapixels with a Sensor size of 1/2.33 inch. The Lens flaunts a 4.00x zoom (25-100mm eq) quality, enabling distant object to appear closer through the lens. With an ISO of 100-1600 and a Shutter speed of 60 (minimum) and 1/1300 (maximum), the Panasonic Camera comes with the dimensions of 4.1 x 2.3 x 0.8 in.(104 x 58 x 20 mm). the Camera weighs about 5.1 oz ((144 g)includes batteries).

Begining by labeling 7 different 2.0 ml tubes 0 thru 6 for each compound. Then add 1ml of extract to tube 0. Then add 0.5 ml of DMSO to tubes 1 thru 6. Now make a 1:2 serial dilution from 0(pure extract) to 6(1:16)

How should the unknown microscopic organisms be classified? The concept of the experiment was to conduct a systematic observation about the traits of unknown organisms, and classify them to the right group. Making detailed observations about the traits, made it possible to identify which cell the trait belonged to because different cells have different traits. Animal cells do not have a cell wall, and has one or more small vacuoles. Plant cells has a cell wall, rectangular (fixed shape), and has a chloroplasts. Cytoplasm, Endoplasmic Reticulum, Ribosomes, Mitochondria, and Golgi apparatus to name a few.

2. (5 pts) List and explain the names and affiliations of the various characters/stakeholders in this story – I’m looking for us to use the story to map out the complexities that are generally associated with solving public health puzzles – the stakeholders you list and explain here should apply to many of the cases we consider going forward.

The Uptake of Neutral red dye by saccharomyces cerevisiae in the presence of a metabolic inhibitor

The first hypothesis was unsupported as Tube 4 (positive control) had the most succinate (.2mL) but ended with the lowest % transmittance out of the three sample we were examining (Tubes 2-4). We believe this discrepancy to be due to the variability of the mitochondrial solution. If the Tube 4 mitochondrial suspension had less concentrated enzyme than the solution for Tube 3 and Tube 2 then Tube 4 would be biologically incapable of matching their rate of reduction. It's worth mentioning that Tube 2 and 3 had a %T change of 23.1% and 23.2% respectively while Tube 4 exhibited a change of only 20.4%; further supporting the idea that Tube 4, despite its increase in succinate concentration, wa lacking in other enzymes/proteins necessary for the

The purpose of this memorandum is to explain the results of the night light project. The experiments for the lab were carried out on November 1st, 2016 with Brian Tran and Brady Lucia as lab partners.

When we write, “drill a 3/8” diameter, 1” depth hole in the center of the dowel”, the reality is a lot different. First, we need to find 3/8” tap size that is specifically designed for wood. Then, we need to find the center point of the dowel and figure out how to drill a hole perfectly centered. The process took us 2 hours in lab to figure out the most efficient and effective way to do such simple task. Because of time constrain, we decided to finish the system later in the day.