Common Fruit Fly Essay

Week 4: The F2 generation flies were present and identified by phenotype and gender. After identification, they were let go and the number of flies with each set of 2 traits were combined.

To set up this experiment, two twenty-five gallon aquariums, 3 petri-dishes, 200 flies, rotten bananas, and yeast were used. The bananas chosen to be an accelerant for the growth of the yeast and were frozen so they would be easier to cut. The yeast was used because the drosophila melanogaster prefer this as a food source.  The vestigial and wild type flies were sexed (to determine their sex), sorted, and counted.  An initial population size of 100 total flies was decided so that it would be easier to determine the phenotypic percentage of the total population. Fly paper was placed in one of the sets of cages to impose a method of natural selection as well as the sexual selection which is being solely tested by the other set of cages.

Drosophila Melanogaster, commonly known as fruit flies, are highly important model organisms in pertaining to biological research. The logic behind their recurrent use is due to their: easy culture in the laboratory, brief generation time, and ability to produce large numbers of offspring. In this report, we created isolated virgin D. Melanogaster from the original three populations we were given and then created crosses between them. Upon observation, we noticed an unusual mutant that arose from two of the three created crosses. We suspected that this genetic mutation had previously been discovered and named.

The CO2 is believed to reduce the PH level of the flies, which causes them to fall into an induced sleep. Regulating the control of CO2 flow was adjusted through a CO2 valve on the lab bench and FlowBuddy device. The anesthetic was manually set at 5L/min and delivered to the flies though a small devise resembling a hot glue gun, and a surface pad which diffused CO2 though small pores. While properly holding the vial at an angle of 70-80 degrees, the acetate plug was rotated and loosened. This allowed room for the tip of the gun to enter into the vial and release CO2. The flow of the anesthetic into the vile was administered for 5-15 seconds while gently pressing the gun trigger. Flies immediately fell to the top of the vile, the plug was removed, flies were placed on the surface pad for observation, the plug was immediately placed back on the vial, and the vial was placed on its side incase flies were still asleep in the

When the larvae hatched, the anesthetising procedure shown from steps 1-5 was used to record the phenotype of the F1 generation and number of male or female flies.

The main purpose of this lab was to utilize the infamous Hardy-Weinberg equilibrium equation to predict the evolutionary modifications a certain species (Drosophila melanogaster) displayed throughout different generations. For this experiment to be carried out, Drosophila melanogaster, also known as fruit flies, were used to visually represent evolutionary conceptions such as Hardy-Weinberg equilibrium equation. At the beginning of the experiment, the parent generation was observed first. Throughout the course of seven weeks, the vial was analyzed for certain changes between the two populations of Drosophila melanogaster; wild type and ebony. Although the genotypes could not be figured out, the flies were evaluated and observed based on

Genes can either be sex-linked or autosomal. If a gene appears mostly in one sex chances are the gene is sex-linked and if it appears frequently in both sexes it is most likely autosomal. Using Drosophila melanogaster, also known as the fruit fly, we will determine whether the gene is sex-linked or autosomal. Drosophila melanogasters have a relatively short life span and are an excellent organism for genetic studies because it has simple food requirements, occupies little space, is hardy, completes its life cycle in about 12 days at room temperature, produces large numbers of offspring, can be immobilized readily for examination and

The first thing that was done in the Fruit Flies experiment was to prepare the food of the obtained fly food vials that were given by instructor Dr.Kang. The preparation to make the fruit flies vial with the food was to obtain a disposable plastic vial in which the fruit flies were maintained. As well as

At the same time, male wild type flies were removed from their vial, anesthetized, and then separated into four groups of 4 or 5. Five new vials of media were made, and the offspring from the P generation were put into four of the new vials. Once again, there were approximately 5 flies of each trait and gender in

The approximately 30 wild type males were and kept alive in order to cross mate with the females. All of the flies from all four vials that were collected were part of the P generation. Crossing the flies was the next

We began the experiment by dividing into pairs and selecting one mutation of flies. Claire and I started off with Vestigial Flies. We then got our supplies; three clean tubes, three caps, three sponges, fly nap and wand, dissecting microscope, paintbrush, and three small plastic plates (for separation). As a team, Claire began to tap the container containing the mutant flies (this way they stayed at the bottom), while I had my extra sponge and clean tub ready to stick on top of hers’. After counting down to three, she pulled the sponge on her fly container and I quickly placed the empty vial on top. I then flipped the vials upside down and proceeded to knock as many to the empty vial as I could. We did the exact same step as before, counted

Introduction Drosphila melanogaster, commonly known as the fruit fly, is an excellent organism for genetics studies because it has simple food requirements, occupies little space, is robust, completes its life cycle in about 12 days at room temperature, produces large numbers of offspring, can be immobilized readily for examination and sorting, and has many types of hereditary variations that can be observed with low-power magnification. The fruit fly has a small number of chromosomes (4 pairs), which are easily located in the large salivary gland cells. As mentioned before, the fruit fly life cycle is complete in about 12 days. First, a fertilized adult female must lay the egg, which leads to the first stage of the fruit fly life cycle, the egg stage.

Then we removed the flies from the freezer within 4 minutes and gently tapped a few of them into the anesthetizing chamber and closed the lid. After 3 minutes we removed the lid and placed the immobilized flies under the light scope and began to separate male from female. Males had a darker end than females making separation easy. Placing two males and two females into each vial at a 90 degree angle to ensure the flies don’t stick to the food on the bottom and die. We then placed the vials on their size into a dark and closed space.

At the end of our experiment, we were able to calculate the error percentage with the actual calculations. We were also able to compare the Punnett squares too. Some of the actual calculations were quite different than the expected, while others were spot on. One example is with normal wings, yellow body, our actual was two thousand two hundred and eighty-four flies and so was our expected. Whether the results were spot on or completely different we still had results to compare to the expected amount of each type of fly.

Drosophila Melanogaster is a fruit fly that is commonly used for genetic studies (reference 2). It is an excellent organism for genetic studies because it is small, inexpensive and easy to culture. It occupies little space, and requires simple food (reference 3). Also, it completes its life cycle in about 10-14 days at 25ºC and It produces large numbers of offspring (reference 3).Moreover, it has abundance of heredity variations, and it has a small number of chromosomes which are easily located in the large salivary gland cells (reference 3).There are four important stages in a Drosophila's life cycle consist of the egg, larva, pupa, and adult. Both Drosophila male and female have noticeable features that distinguish them apart.