Common Microorganisms Essay

6 Wash your hands again. Then clean the stoma area as directed with water or with mild soap and water. Use water to rinse away any soap.

6. The disks in the 0.00% solution were transferred to an agar plate held next to the blue flame using the sterilized tweezers. Excess disinfectant was removed from the disks by wiping on the side of the well of the spotting tile. When the 5 disks were positioned (refer to Figure 1 below) the lid was replaced and sticky taped down. A label was added indicating the concentration of disinfectant.

In the beginning of lab, we were advised to obtain a nutrient agar petri plate, which is used for the cultivation of microbes supporting growth of non-fastidious organisms. Since it contains many nutrients, a wide variety of bacteria and fungi can grow. Taking the plate,

| UNIT 4222- 264 THE PRINCIPLES OF INFECTION PREVENTION AND CONTROL | | | |

Health Promotion: Patient regularly cleans the ears with Q-tip cotton swabs, and avoids any exposure to any environmental noise hazards.

Read in your lab manual about the following agar mediums: Blood Agar (pg 168), EMB Agar (pg 170), Mannitol Salt Agar (MSA)(pg 172) ), MacConkey Agar (pg 174), and PEA Agar (pg 176) to answer the following:

4. Exposure 3 repeat step 3 w/o including the saline bags (used as a control).

In addition to diligent hand washing and the consistent use of other Standard Precautions, which measure will help reduce the transmission of Hepatitis?

Since microorganisms are not visible to the eye, the essential tool in microbiology is the microscope. One of the first to use a microscope to observe microorganisms was Robert Hooke, the English biologist who observed algae and fungi in the 1660s. In the 1670s, “Anton van Leeuwenhoek, a Dutch merchant, constructed a number of simple microscopes and observed details of numerous forms of protozoa, fungi, and bacteria” (Introduction to Microscopes, n.d.). During the 1700s, microscopes were used to further explore on the microbial world, and by the late 1800s, the light microscope had been developed. “The electron microscope was developed in the 1940s, thus

Please complete the entire experiment as instructed in the lab manual except for any modifications noted below. Fill out the

Samples were collected from the tabletop, the camera, the window and the refrigerator door. The samples, were then placed in the petri dish, in its designated segment. The other petri dish had samples from the mirror, the stethoscope, the doorknob, and the steering wheel of the car.

An eyewash station should be present within the laboratory in case of splashing of infectious agents in the

2. Introduction: Each student was given unknown bacteria and was instructed to perform a variety of experimental tests that would help to identify their bacteria. During the process of identification, the unknown bacteria was added to many different testing medias using aseptic technique. They are as follows: lactose fermentation on eosin methylene blue (EMB), TSI (Triple Sugar Iron agar), Phenol red sucrose, the SIM test, H2S by SIM, IMViC (indole, motility, voges-proskauer, and citrate), Urease (urea broth), PDase (Phenylalanine Deaminase), Lysine Decarboxylase, and Ornithine Decarboxylase. Colonial morphology on EMB was used to

Life on this planet began with microorganisms. Through millions of years microorganisms have found ways to successfully adapt and survive. These adaptations have created a wide biodiversity, allowing them to basically populate in all places. Why are these microbes so important? Because they shape the history of our world. Some microbes can be deathly to humans while some others are favorable, for example, bacteria that lives in the gut of both humans and animals and helps during the process of digestion (Alfred Brown & Heidi Smith, 2006). Understanding these interactions help scientists to find ways to protect humans from potential deathly pathogens. In order to observe microbes, microscope proficiency and microorganisms’ identification are crucial skills in a microbiology lab. During this laboratory session, samples of environmental and human organisms were inoculated into two different rich media and incubated to their according temperature. After this, appropriate use and calibration of the microscope was performed. Lastly, morphology and size of different species of bacteria, algae, fungi and protozoan were recorded.

Introduction: Through the conduction of numerous experiments, the identity of two bacterial isolates was determined. The tested specimen was an unknown sample of a mixed culture of two different species of bacteria. The first step that was taken was obtaining a pure culture of each species of bacteria by isolating one species from the other. Once isolation was complete, the isolated cultures were tested using procedures that had been performed during previous lab sessions. A gram stain was performed on the two isolates. The isolate which had tested gram negative was then tested for the presence of cytochrome C and lactose fermentation. For the gram positive isolate, cell shape was determined and a catalase test was performed.