Comparative Analysis Of Antibiotic Growth Of Staphylococcus Aureus

Methicillin-resistant Staphylococcus aureus, or more commonly, MRSA, is an emerging infectious disease affecting many people worldwide. MRSA, in particular, is a very interesting disease because although many people can be carriers of it, it generally only affects those with a depressed immune system; this is why it is so prevalent in places like nursing homes and hospitals. It can be spread though surgeries, artificial joints, tubing, and skin-to-skin contact. Although there is not one specific treatment of this disease, there are ways to test what antibiotics work best and sometimes antibiotics aren’t even necessary.

Methicillin Staphylococcus aureus is defined as strains of bacteria that are resistant to beta-lactam agents, including the synthetic penicillins (eg, methicillin, oxacillin) and the cephalosporins. MRSA can resist the effects of many common antibiotics, so it is difficult to treat. If the infection spreads to the blood stream and becomes systemic may increases risk for life threatening complications. First sign of MRSA are small red bumps that resemble pimples, boils or spider bites. Next they may mature into deep and painful abscesses that require surgical draining. If the bacteria is not confined to the skin and infect the blood stream, causing potentially fatal infections in bones, joints, surgical wounds, the bloodstream, heart valves and lungs.

Methicillin-resistant Staphylococcus aureus, or MRSA, is a bacterium that is resistant to many available high- level beta-lactam antibiotics, which include penicillins, “antistaphylococcal” penicillins and cephalosporins. Staphyloccoci are the most important bacteria that cause disease in humans. MRSA is sometimes branded as a nosocomial infection as it usually plagues patients that are receiving treatment in a hospital. The skin is the most common site of infection, where MRSA can cause cellulitis, folliculitis, or boils in the skin, but can also commonly be found in the nose, underarm, groin, upper respiratory tract, intestine, vagina and rectal areas of the body. MRSA infections occur with there is a break in the skin that allows the Methicillin resistant

Staphylococcus aureus (S. aureus) is a spherical bacteria which is known to produce a cytotoxin called Panton-Valentine leucocidin (PLV) which destroys leukocytes, and kills tissue (Lina et al., 1999). Five percent of strains of Staphylococcus are known to produce the disease-causing toxin (Lina et al., 1999), but though the amount of PLV-producing strains is somewhat small, the strains which produce PLV are apparently resistant to vancomycin, an antibiotic commonly used to treat staph infections (CDC, 2002). The first recorded case of S. aureus resistance to vancomycin was a reduction in sensitivity to the antibiotic observed in Japan, and has since spread to the United States (CDC, 2002). The most common source of infection of these drug-resistant bacteria are actually in hospitals, wherein the patients are exposed to the bacteria and subsequently infected (CDC, 2002).

1 and 3). This antibiotic is effective against both gram positive and negative bacteria, so it would be effective against Staphylococcus epidermidis. This antibiotic is able to kill bacteria by interfering with the cell wall synthesis, weakening it, and causing its death. However, just like with Penicillin, some bacteria have become resistant to this antibiotic (6). The hypothesis stated that Staphylococcus epidermidis would be susceptible to Ampicillin because although some bacteria have become resistant this one has not (7). According to the class data, two of the tests resulted in in susceptibility, one resulted in an intermediate effect, and the rest were resistant (see Figs. 2 and 5). It seems that Staphylococcus epidermidis is in the process of becoming resistant to Ampicillin because of the varied data. However, the group data did not support the hypothesis that the bacteria would still be

From a swab from the MSA plate a lawn of bacteria will be made on a Muller-Hinton plate. Four evenly spaced antibiotic disks will be placed on the plate. The plate will be left to incubate. After an incubation period the diameter of the zone of clearing will be measured to determine the resistance of the bacteria to the antibiotics. This test is not used to determine the bacterial species; it is just used to discover what antibiotics will be the most effective. Knowing all one can form a hypothesis of this experiments outcome: it is very likely if the swabbing of the nose and remaining tests are executed properly that there will be a species of Staphylococcus detected. If the MSA plate is turned yellow by the pH indicator its probable that the species Staphylococcus aureus specifically was detected, this will be proven or disproven by the antibody agglutination test. If the MSA plate maintains a red color its still a species of Staphylococcus the specific species is just unknown. The gram stain should show a purple in color cocci clustered bacterial formation. As Staphylococcus is gram positive and has a cocci cluster

When penicillin was released to the public in 1944, it was a miracle drug. Infections that had been killers were suddenly treatable. Doctors recommended it generously, both for illnesses that needed it and illnesses that didn’t. Before long, however, it took much stronger doses to see penicillin’s effects. When the antibiotic arms race began in 1944, most physicians assumed that new antibiotics would be discovered or created to keep up with the evolving resistance in bacteria, but the bacteria are constantly evolving new defenses and doctors are starting to run low on antibiotic ammunition. MRSA, methicillin-resistant Staphylococcus aureus, is one of many types of bacteria

The antimicrobial susceptibility patterns of S. aureus (MRAS and MSSA) isolates to various antibiotics are presented in Table 1. All S. aureus isolates were susceptible to vancomycin. Quinupristin-dalfopristin, linezolid and minocycline with resistance rates of 1.1%,

Methicillin-resistant Staphylococcus aureus is not uncommon in hospitals and other healthcare facilities. It is one of the top, most problematic nosocomial infections currently being seen, and battled. A newer strain of MRSA, Community-Associated, is now becoming almost as prevalent as the Healthcare-Associated, and is continuing to grow substantially in number. With Community-Associated Methicillin-resistant Staphylococcus aureus (CA-MRSA) on the rise in the United States, and rapidly gaining on the more common Healthcare Associated MRSA, the need for scientists and doctors to not only define / classify this gram-negative bacteria, but also to learn how it is transmitted, the characteristics promoting its survival, and how to prevent the spread

Three of the four quadrants were labeled S, P, T, for streptomycin, penicillin, and tetracycline. One quadrant was left blank for the control (Figure 1.1) Having a control of no antibiotic was used to show the growth of the bacteria when it is uninhibited by antibiotics. Then, Escherichia coli K12 from VWR (catalog #470179-082) and Staphylococcus aureus from VWR (catalog #470179-208) were evenly swabbed across the surface of the agar plate with a cotton swab; ten plates, contained E. coli and the other ten plates contained S. aureus. Then, Streptomycin (10 μg/mL), Penicillin (10 U = ~10 μg/mL), and Tetracycline (30 μg/mL) were obtained and placed into the middle of their respective quadrants. After this, the agar plates were stacked into two stacks of five, for each bacteria, were wrapped with parafilm, and were incubated at 37°C for 48 hours (Figure 1.2). After 48 hours, the plates were moved to a refrigerator at 4°C, until they were taken out of the fridge approximately a week after the stacks of agar plates had initially been placed in the incubator. The diameters of the zones of inhibition were then measured with a ruler and recorded to the nearest millimeter. After measuring the zones of inhibition, a gram-staining technique was used to illustrate the difference in cell wall structure between S. aureus and E. coli. First, a small sample of each species of bacteria was transferred with a loop from the agar plate onto two different areas on a microscope slide that had previously been washed, wiped with alcohol, and dried. Then a drop of water was added to each bacterial sample, was smeared into a thin layer, then allowed to air dry. Once the bacterial samples were dry, the slide was held at one end and was passed through a Bunsen burner flame with the smeared-bacteria samples facing up.

Staphylococcus aureus is a leading cause of human bacterial infections worldwide1, and following the discovery and widespread utilization of antibiotics, S. aureus has evolved to become resistant to a number of antimicrobial treatments. Most notably, Methicillin-resistant Staphylococcus aureus (MRSA) strains have acquired the mecA gene, encoding the penicillin binding protein (PBP2a), which confers resistance to oxacillin and all β-lactam antibiotics 2. These characteristics, combined with other virulence factors, have made MRSA infections difficult to treat, and lead to MRSA being recognized as a significant cause of morbidity and mortality 3. MRSA is also the leading cause of nosocomial infections4–6, contributing significantly to increased healthcare costs 7. CDC estimated in 2008 that MRSA was responsible for 89,785 cases of disease, causing 15,249 deaths in the US8.

When Penicillin was first introduced in the early 1940’s to treat bacterial infection, resistance strains of Staphylococcus Aureus were completely unidentified. However, only a decade later, the disease was already becoming very common in hospital environments. Because of this, Methicillin was introduced in 1961 to medicate these resistance strains, yet within a single year, doctors were already encountering Methicillin-Resistant Staphylococcus

Methicillin-Resistant Staphylococcus aureus (MRSA), a pathogen that causes many complicated health infections in our body. It’s a type of s. aureus bacteria that can produced by process of resistance to many antibiotics such as dicloxacillin, oxacillin, and methicillin. Why is MRSA so dangerous? Its natural ability is to continue transforming in ways to prevent from antibiotic from being completely successful in the batter against MRSA. The US had spent billions of dollar every year into research in order to prevent it from further spreading. In order to do so, we must step up to advance our technology and knowledge to be able to defeat the bacterial warfare. This research will discuss the prevalence and different factors of the disease, it

This experiment was done to better understand what types of antibiotics do well against the strain of S. aureus, MRSA. The experiment consisted of various types of antibiotics to see if there would be any

Modulations of antibiotics policies alone do not ultimately eliminate MRSA from clinical settings. Thus, another method for the cytoxicity of MRSA is described by Von Eiff et al. They describe how S. Aureus can be killed in vitro by shock-waves. They also suggest osmolyte stimulation of innate anti-microbial defence systems and bacteriophhage therapy to eliminate the weaker strains of MRSA. Therefore, concentrate efforts on a narrower range of MRSA so MRSA as a whole is more likely to be significantly reduced if not eradicated completely.