The Relationship of Triclosan to Bacterial Colonies as found on human hands
Group 3
Biology 135-002
Introduction In our experiment, our goal was to provide data to show whether soaps with triclosan were more effective at removing bacterial colonies than soaps without it. Tests were done on two people of the group with each individual soap provided while one other person functioned as the control for the experiment by not washing their hands at all. By doing this, you are able to compare what natural, unwashed hands look like in relation to the two types of soaps used on our two other subjects (one of which contains triclosan). We hypothesized that hand soap with triclosan is more effective at eliminating bacteria as compared to regular hand
Ajax, which was thought to have killed the most E. coli, was because of its ingredient lactic acid, which was used as a natural anti-bacterial. A potential reason why the Ajax didn’t kill as much E. coli as what was predicted might have been that the amount of Ajax solution soaked up was not actually sufficient enough to cause an effect. Therefore, the results varied. The results were different and had a large gap between them causing three varied answers. Vinegar was thought to kill not as much as Ajax but as it turned out, had a larger zone of inhibition but didn’t efficiently kill or clear as much. The vinegar tried to kill more because of the high acid levels that entered the cell membrane and killed the cell. The vinegar that was used didn’t kill as many cells because the concentration was not high enough. Again, the same as the ajax, there were 3 varied answers. This is proposed to be because of different of length of time that it spent in the solution. Salty water which was presumed to kill the least was correct.
The purpose of this experiment is to investigate the effects of various household products may have on bacterial growth. The bacteria that was chosen as a test subject is Micrococcus luteus, which is generally considered a harmless bacterium unless the host has a weakened immune system. Among the household products that a set of groups will test are common cleaning and disinfecting products such as rubbing alcohol, Betadine, Germ-X, Neosporin, liquid hand soap, and Lysol cleaner. In the experiment, the bacterium, Micrococcus luteus, is to be swabbed and grown in a Petri dish divided into four sections with agar in each. After that, a small circle of thin paper is soaked in the four household items. Then, a piece of parafilm
THE EFFECT OF INCREASING THE CONCENTRATION OF DETTOL ANTISEPTIC LIQUID ON THE GROWTH OF E.COLI
4. Based on these results, what is the importance of soap and time for effective hand washing?
The aim of this investigation was to find out which antiseptics were most effective at preventing the growth of bacteria.
Visibly soiled hands (bodily fluids, etc.) should and must be washed with soap and water to substantially rid the hands of microbes. Studies found that hand sanitizers with an alcohol content of 60-95% can kill germs effectively (instead of hand washing) compared to those hand sanitizers that contain less than a 60% alcohol concentration or those without alcohol. Growth of microbes or bacteria present on the hands may be inhibited, but will not completely rid their presence. They may still stay on the hands and not completely “wash off,” like that during the process of rinsing during hand washing where water will rinse them off the hands and into the sink. Bacteria like Clostridium dificile or E. coli are familiar organisms present in the healthcare settings and may not be effectively removed by hand sanitizers (those with alcohol content) alone. They may still be found in the crevices of the hand or ridges in between the fingers and can sometimes spread to the wrist area and forearm if not properly
The purpose of this study is to evaluate the impact of hand hygiene and how it decreases the transmission of infection throughout (Bloomfield, Aiello, Cookson, O'Boyle, & Larson, 2007). Handwashing can include alcohol based hygiene items and handwashing with soap and water. This study main focus was on North American and Europe. There is plenty of supporting rationale to backup why this study was conducted. Some of the few things this study wanted to achieve are hand hygiene is key to staying healthy and reducing infection. This needs to be followed both in the workplace and around the community to abstain from infections. Handwashing can be achieved by soap and water or hand sanitizers that removes or eliminates many microorganisms on the surface of the hand (de Oliveira Dourado, da Costa Barros, Diogo de Vasconcelos, & da Silva Santos, 2017). This can impact many individuals by using this technique to keep foreign germs off of the hands. The importance of washing hands
This experiment illustrates the importance of handwashing and proves that hand washing is worth it. Since our hands are constantly coming into contact with ourselves and others, touching surfaces, grabbing objects, being sneezed into, etc., keeping our hands clean is one of the most effective, yet simple way we can take to avoid getting sick and spreading germs to others. Many diseases and conditions are spread by not washing hands with soap and clean, running warm water. “The human skin is a host to anywhere between 10,000-10,000,000 bacteria per square centimeter and since health care providers come into contact with pathogenic bacteria by being engaged in patient care, hand washing can reduce the risk of spreading diseases (page 3).” The objective of the experiment is to test the effectiveness of hand washing and demonstrate normal flora. This report presents the procedures and materials for the experiment, the experiment's results, and an analysis of those results.
The main objective of this experiment is to investigate the effect of different types of antibiotics on bacteria Bacillus subtilis and Escherichia coli. Some of the main methods used in this experiment
This assignment will encompass how the results of the personal hygiene and susceptibility microbiology experiments provide a framework for the basis of the NICE Clinical Guidance (CG139) on Infection and how crucial the reasons for the hand wash protocol and hygiene is in all healthcare settings.
The other side shown in this article is the studies done in schools that are instituting hand washing programs school wide. These studies show:
Rob Dunn, a contributor to Scientific American Blog, states, “Increasingly, the world seems to be rife with contamination. Fortunately, all of the main companies producing hygiene products have offered a solution–sanitary, antibacterial, antimicrobial, antibiotic, wipes, and soaps to kill anything that dares to creep into our wholesome lives. These salves will cure us of the demons that dare to grow near us.” When Dunn says this, he is completely correct. Without the proper and frequent use of sanitation products, such as soap, the general public would be more exposed to the world full of deadly germs.
At the University of Saint Joseph there is a 40 to 60 ratio between residents and commuters. The resident’s dorm provides communal washing machines and dryers. There are 6 washing machines in Madonna and 4 washing machines in Assumption. There are approximately 164 residents that share 6 washing machines in Madonna and 81 residents in Assumption that share 4 washing machines. (Residence Halls) Washing machines clean clothes, but what cleans the washing machines? If washing machines are not properly cleaned or not properly used can lead to different microbes to grow. (Carollo) In a study of 50 homes in Tucson and 50 homes in Tampa Bay Florida they found coliform bacteria, and E. Coli that caused diarrhea in many of the washing machines. (Rostein). From further investigation they discovered that 60% of the washing machine had coliform bacteria and 10% had E. Coli in it. (Rostein). “Researchers found that 40 percent of sterile cloths washed in non-bleach laundry contained fecal bacteria. In fact, there was enough left over to contaminate the next wash load” (Rostein) Bacteria such as Salmonella, and viruses including hepatitis A, rotavirus and adenovirus where also found in the washing machines. (Rostein) “While E. Coli was killed in the permanent press drying cycle, some salmonella survived on clothes at 131oF.”(Rostein) In this project, this group will be testing to see if the facilities and students are
Curtis et al (2001) noted that modern methods of promoting handwashing can be effective and cost-effective on a large scale. Studies suggest that soap is widely available, even in poor households in developing countries, although it is mostly used for bathing and washing clothes (Borghi et al, 2002). In rural India and Bangladesh, soap is often considered a beautifying agent or for the physical feeling of cleanliness which it gives, rather than being associated with the removal of microorganisms or health benefits (Hoque and Briend, 1991; Hoque et al, 1995).