Comparison of the Mechanical Properties of Fresh and Cryopreserved Aortas

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Considering the results, one could conclude that freezing has an affect on the toughness properties of the samples. Although, the quantitative mechanical properties changes by freezing the samples, the qualitative behavior of the samples does not show any obvious changes in the toughness properties, which is a macro-mechanical property.\\ This study has a number of limitation that has to be considered. First of all, although the fresh-tissue samples were collected and tested shortly after postmortem, the fresh-tissue samples were dead and their mechanical properties could be different in comparison to live tissue samples in the body. Moreover, the sharpness and thickness of blades, the blades' angles and the type of lubrication affect the fracture toughness values. In order to make the sharpness of blades steady-state, the blades used in the experiments were preconditioned by passing through the discarded porcine tissue at least twenty times before the tests. GEM single edge 0.009” aluminum-back razor blades were used. Different types of oils were tested and the one causing the lowest amount of friction were selected for the experiments. Furthermore, the consistency of the crack tip condition was assured by means of utilizing the cutting blade constraining the crack tip configuration. However, crack blunting and fiber pullouts cause toughening in the physiological conditions. The next step of this study could be to study the histological variations between the frozen and

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