Concentrations of Bile Salts on Lipase Enzyme

Enzymes are biological catalysts that speed up chemical reactions, without being used up or changed. Catalase is a globular protein molecule that is found in all living cells. A globular protein is a protein with its molecules curled up into a 'ball' shape. All enzymes have an active site. This is where another molecule(s) can bind with the enzyme. This molecule is known as the substrate. When the substrate binds with the enzyme, a product is produced. Enzymes are specific to their substrate, because the shape of their active site will only fit the shape of their substrate. It is said that the substrate is complimentary to their substrate.

Temperature controls the speed the enzymes work at. Higher temperatures increase the kinetic energy which increases the chance of collision therefore speeding up the rate of

I predict that at temperatures above 70°C the enzyme lipase will become denatured and at temperatures below 10°C the enzyme will become inactive. Since lipase operates within the human body I’d also predict that its optimum temperature would be around human body temperature which is approximately 37°C. I predict that before the optimum temperature the rates will gradually increase and preceding the optimum there will be a drastic decrease in rate until the enzyme is denatured.

Enzymes are proteins which can catalyse chemical reactions without changing themselves. The enzyme lipase breaks down the fat in dairy products such as full-cream milk for people who are lactose intolerant. Lipase acts on its specific substrate, lipids produces fatty acids. If enzyme concentration increases, random collisions between the substrates and active sites of enzyme increase due to the increasing amount of active sites which allow more collisions to happen, so the rate of breakdown of lipids to simpler substances will increase. During the experiment, sodium carbonate solution and pH indicator phenolphthalein will be added ahead of

Experimental Optical density (OD) of colored filtrate (reflects amount of bile 1 - OD (reflects amount of bile acid adsorbed by food particles)

Enzymes are a very important to the biological process. Enzymes help break down food and are essential in helping convert that food to energy. Enzymes have a single function, which makes them unique and need specific conditions in order for the reaction to occur. Every function in an organism has its own unique enzyme (What are enzymes?). One important thing to know about enzymes is that they are proteins. According to rsc.org enzymes are efficient catalysts for biochemical reactions and they, “speed up reactions by providing an alternative reaction pathway of lower activation energy” (Enzymes).

Substrate concentration also affects the rate of reaction as the greater the substrate concentration the faster the rate of reaction and all the active sites are filled. At this point the rate of reaction can only be increased if you add more enzymes in to make more active sites available.

Bile is a green-colored liquid used for digestion that is created by the liver and stored by the gallbladder. There are bile acids (bile salts). They are used to absorb fats and for digestion. The absorption of fats happens in the small intestine.

There are thousands of chemical reactions that occur in an organism that make life possible. Most of these chemical reactions occur too slowly on their own. Enzymes are protein catalysts that speed up chemical reactions in a cell. Catalysts are not changed by the reactions they control, and are not used up during the reaction. Enzymes therefore, can be used over and over again. Enzymes are large complex proteins made by the cell and allow chemical reactions to take place at the temperature of the cell. These catalysts are needed in only very small amounts because a single enzyme molecule can complete the same reaction thousands of times in one minute.

The gallbladder stores and concentrates bile. If the gallbladder was damaged or dysfunctional there would be softer and more frequent stools. (Audesirk, T., & Audesirk, G. (1999). Retrieved November 21, 2015 from Chapter 29: Nutrition and Digestion. In Biology: Life on Earth (5th ed., p. 585, 586). Upper Saddle River, N.J.: Prentice Hall.) (General Surgery- Gallstones (Cholelithiasis). (n.d.). Retrieved November 21, 2015, from

Describe the mechanism through which the secondary bile acids resulting from microbial action relate to the risk of colorectal cancer.

Enzymes are biological molecules that are in all organisms. They act as catalysts, which help speed up a reaction without being damaged during the process. Enzymes are extremely useful to organisms because they help the metabolism function (Berg, 2002). They are not all over the place in the body; however, they are in the cells where they make cellular digestion possible. Enzymes act upon substrates where they fit like lock and key as if they are complementary shapes (Hillis, 2012, pg. 51).

Terms Enzyme: A protein that acts as a catalyst, lowering the activation energy needed for reactions to progress in cells. The reaction can still occur without the presence of the enzyme, but at a much slower rate (Enzyme, 2015). Substrate(s): The reactant(s) of the reaction that is/are bound by the enzyme (Subtrate, 2015). An “inactive” and “active” reaction of an enzyme.

The bile moves into the gallbladder via tiny tubes. The bile is stored in the gallbladder and waits, becoming concentrated, for the signal to be released into the duodenum aiding in digestion. Without bile, the body could not digest fats, as fats do not absorb into water. The bile acts as a detergent and allows the two to mix.

This substance is produced when a human digests lipids or fats. According to Sareen Gropper and Jack Smith’s “Advanced Nutrition and Human Metabolism” bile is produced through the process of chemically altering cholesterol, which is a type of fat found in a human’s blood. This process takes place in the liver and bile acids are produced. These bile acids are then conjugated or united with taurine and glycine to produce biosurfactants known as bile salts. The existence of bile salts inside of bile gives the substance the capacity to amalgamate lipids and fats with their aqueous (watery) environment inside of the gallbladder. According to Sareen Gropper and Jack Smith’s “Advanced Nutrition and Human Metabolism” bile is released from its origin (gallbladder) into the small intestine. Through the process of emulsification the bile substance in the small intestine disintegrates fats into much tinier particles which can be digested and absorbed at a much more efficient and quicker rate. This evidently shows that without bile and in turn bile salts the digestion of fats will not be