Conditioning of Bacteriophages to Combat Common Disease
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Conditioning of Bacteriophages to Combat Common Diseases through Generational Selection and Isolation
In 1928 Scottish biologist Sir Alexander Fleming discovered the remarkable antibiotic penicillin. This drug revolutionized the medical world with its ability to combat infectious disease which had for so long plagued mankind. Though still used today nearly a century later, penicillin and other antibiotic drugs like it are rapidly becoming ineffective at combating diseases. The reason why is like other organisms bacteria have the ability to adapt and evolve to survive hostile environmental factors, which in this case means they are becoming resistant to antibiotics. So the problem society is faced with is how to replace these revolutionary drugs like penicillin which are rapidly becoming ineffective; the answer is bacteriophages. Bacteriophages (or viruses) are the hunters of the microbiome; and unlike some other antibiotics they have the ability to change and adapt with bacteria in order to stay relevant. What will be attempted in this lab is to condition bacteriophage through artificial selection over multiple generations to become more effective in fighting specific species of bacteria.
Collection of a Novel Phage from the Environment
We collected 5 soil samples on campus (Latitude: N 33° 44' 55.8269" Longitude: W 84° 24' 54.6172") and placed them in a 15-mL conical tube. For this procedure we wanted to create a suitable nutrient rich environment for the host