Essay Constructing Complex Synthetic Gene Circuits

2005 Words9 Pages
Regulation of any biological phenotype is a complex and tuned phenomenon (Weng et al, 1999). Biological systems face frequent change in the environment, both at intrinsic and extrinsic levels. These systems have evolved themselves to integrate these fluctuations and take an appropriate decision (Helikar et al, 2008). Classical genetics and DNA sequencing have provided a lot of information about different types of genes and their functions, but how do these genes interact and integrate environmental signals to encode different phenotypic responses, is not clearly understood. Emergence of synthetic biology has enabled biologists to characterize these genetic components in isolation and predict the behavior of complex networks. Synthetic…show more content…
Regulation of any biological phenotype is a complex and tuned phenomenon (Weng et al, 1999). Biological systems face frequent change in the environment, both at intrinsic and extrinsic levels. These systems have evolved themselves to integrate these fluctuations and take an appropriate decision (Helikar et al, 2008). Classical genetics and DNA sequencing have provided a lot of information about different types of genes and their functions, but how do these genes interact and integrate environmental signals to encode different phenotypic responses, is not clearly understood. Emergence of synthetic biology has enabled biologists to characterize these genetic components in isolation and predict the behavior of complex networks. Synthetic biology based approaches have been implemented to construct circuits mimicking natural circuits and understand the design principle of these circuits (Bashor et al, 2010; Rai et al, 2012). Synthetic gene networks have also been designed to perform several tasks including toggle switch, memory, oscillation, logic, biotechnological application (Danino et al, 2010; Gardner et al, 2000; Kobayashi et al, 2004; Siuti et al, 2013; Xie et al, 2011) One of major hurdles in constructing complex synthetic gene circuits is availability of limited number of well characterized regulated promoters and cognate regulators. Most of the circuits have been constructed using PLac, PTet, PBAD and PcI promoters. Recently discovered, engineered CRISPR/Cas, TALE, and

More about Essay Constructing Complex Synthetic Gene Circuits

Open Document