Fig 3C: Anaphase, 3. Switch to high power and center your slide in the apical meristem region, so that you have a field of view in which there is a wide selection of cells in various stages of mitosis (including interphase). Be sure to adjust your light for optimum viewing. Refer to Figure 3A, 3B, 3C: Stages of mitosis in onion root tip cells for guidance.
This is because the plant was in the test tube by itself, and placed in the light so the elodea could freely photosynthesize, which would eliminate a large portion of the carbon dioxide within the tube.
PART 3: How many cells in each stage: Take one view of the Onion root tip and count how many cells you see for each stage (you have to see ALL stages – ‘0’ is not an option – switch views if you cannot find all stages).
Cellular Respiration Lab Report I.Introduction In this lab we are measuring the amount of oxygen used in both germinating and non germinating peas. We are measuring the oxygen consumption by taking a reading of a respirometer submerged in two water baths. The first bath will be cold water and the second warm
Joshua Elekwa Introduction to cell Biology, Fall 2014 Mount Royal University ABSTRACT The cell membrane (Plasma membrane) functions to provide cell support, cell stability and control entry and exit of materials from the cell. This study was conducted to test the effects of environmental conditions such as the on beet root cell membrane (Beta vulgaris). Five trials using varied pH concentrations were tested and absorbance rates were monitored. The experimental results showed that the protein function decreased sequentially when the pH decreased. This allowed the betacyanin dye to leak out which created the color that was needed to determine the intensity and therefore the effect of the circumstances. This supported the hypothesis that the more acidic or basic the environmental condition around the beet cell, the more permeable the, membrane indicated by color intensity. Pigment leakage in the solution was analyzed by using a spectrophotometer.
The purpose of this lab was to see which level of light (measured in lux) made Spinacia oleracea (Spinach) leaf disks float the fastest. Our hypothesis was that an increase in light intensity will decrease the time it takes Spinacia oleracea disks to float. If light intensity is increased, then the time it takes Spinacia oleracea disks to float will be decreased. The mean for the no light (0 Lux) sample and the low light (4 x100 Lux) sample was 1200 seconds with no standard deviation because none of the disks in these two samples floated. The mean and standard deviation for the medium light (110 x100 Lux) was 902 seconds +- 84 seconds. The mean of the high light sample (410 x 100 Lux) was 692 seconds with no standard deviation because only two Spinacia oleracea disks floated so there was no need to measure the variability of the data. The final results indicated that the highest light intensity led to the quickest rise of Spinacia oleracea disks, supporting our hypothesis.
Experiment 1 (Assignment 3): Using sciccors, leaves from the Geranium plant were cut (Plant A was the bigger leaf and Plant B was the smaller leaf). Then begin to heat up the hot plate to boiling temperature of 100℃. Next one beaker was filled with ⅔ of water and another beaker was filled with ⅓ alcohol. Place the beaker of water onto the hot plate until boiling. To speed up the boiling process put boiling chips into the beaker. Then put Plant A (the leaf exposed to air) into the boiling water for 3-5 minutes. After time is up, using tongs, place Plant A directly into the alcohol solution for another 3-5 minutes. When time is up, take out Plant A and place it into a clean petri dish. Once the plant is properly placed, cover the leaf completely
Making the onion tip root cell slide was successful. Our results supported the hypothesis because we saw cells in the onion root tip in prophase, metaphase, and anaphase. As we went up in power objectives, each phase of the cell became more definitive. The cell root was a great indicator of the structures of the different cycles of the cell. This is important because we will be prepared for future labs working with the microscopes and can now adjust it for the best view of the slide. We practiced working with the compound light microscopes and different phases of the cell cycle. Onion root tips are useful to observe mitosis because the cells are frequently diving as the root grows. So when we stained the cell, we caught many cells in different phases. The significance of this lab was to better understand the process and stages of mitosis and meiosis and compare and contrast the mitotic process in plants and animals. We grasped the concepts of what the chromosomes look like, and what they look like in each step of the processes. Having read much about mitosis and meiosis, seeing these cells was the real application of describing and understanding the stages.
Aim: To observe mitosis in onion root tip cells and record the different phases of mitosis.
d. Mitosis- The cell actively divides. Define the following terms: a. Chromosome – Threadlike bodies that carry genes in linear order b. Chromatid – The new DNA molecules c. Centromere- The structure that holds together the new DNA molecules d. Helix- spiral polymer of nucleic acids. When studying the onion root tip slide, where will you focus to find the most dividing cells? The zone of continual cell division called the
Maggie Morris Leaf Disks: The Rise from Bottom to Top ABSTRACT This experiment demonstrates the effects of pH on the rate of photosynthesis by examining the behavior of leaf disks in different pH solutions under light. In this experiment, we used five different pH levels: pH 5, pH 6, pH 7, pH 8 and pH 9. These solutions were created using a combination of hydrochloric acid and sodium hydroxide. Spinancia olcerea or spinach, leaves were used in the experiment to examine the effects of pH on the rate of photosynthesis. The rate of photosynthesis was measured by counting the number of leaf disks that rose to the surface of the solution after each minute. In acidic solutions, the rate of photosynthesis increased while in basic solutions, the rate of photosynthesis decreased.
INTRODUCTION Plants are organisms that can reproduce sexually through meiosis and create more cells through mitosis (Russell et al. 2013). For studying mitosis, the common onion is an ideal choice. Because it is easy for onions to germinate without soil, it is easy to control any substances provided to the plant. The onion root tips are only a few cells thick and grow quickly making them ideal for time efficiency. The onion root tip needs to be squashed between the cover slip and the microscope in order to reduce the slide preparation’s total depth. To dye condensed chromosomes, such as those undergoing mitosis, a stain is used to make
The observation under the microscope of a cell of an onion skin soaked for 15 minutes in distilled water showed that the cell membrane was pressed against the cell wall. The cell
Objectives: To study the effects of hypotonic, hypertonic and isotonic solutions on plant and animal cells. Materials and Apparatus: * Microscope * Electronic balance * Stirring rod * Small knife * Glass slide and cover * 250, 500 ml beakers
This data shows a strange outcome, in the hypothesis; it says that “If acid is introduced to the seed during germination, then the roots will not grow as long as the seeds that are given water”. This statement proves to be untrue, because the roots grew longer with stronger acid than weaker acid, and in some, cases, grew better with strong acid than it did in water. This may be true because of the acid growth theory. The acid growth theory states that auxins cause the elongation of stem cells by promoting wall loosening. It was determined that this wall loosening is caused by hydrogen ions. This idea and subsequent supporting data gave rise to the acid growth theory, which states that when exposed to auxins, susceptible cells excrete protons into the wall at an enhanced rate, which in turn decreases the pH in the wall. The lowered wall pH then activates the wall loosening process which is essentially doing the same thing as the auxin hormone.