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Copper Release Essay

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METHODS AND APPROACH
A. Research Objective #1: Determine the Rate and Amount of Copper Release from Treated Wood by Fungal Exposure to Four Copper Tolerant Brown-Rot Fungi

Introduction. In this objective, rate and amount of copper release will be measured while fungus is growing on copper-based treatments. The working hypothesis is that the selected four brown-rot fungi (Postia placenta, Serpula lacrymans, Gloeophyllum trabeum, Fibroporia radiculosa) have different copper removal degrees in ACQ and CCA treated wood. I will test my working hypothesis by using the experimental approach of measurement of copper release. Fungus growth will be conducted on ACQ and CCA treatments, and percentage of copper release will be analyzed by Inductively Coupled Plasma (ICP). The rationale underlying this objective is that without the analysis of copper release from treated wood, it is highly unlikely
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For this, copper will be determined at the beginning of the study in treated samples which will be reserved for ICP analysis. The samples which are exposed to fungus will be analyzed to determine the percentage copper release after extraction. These exposed samples will be microwave-digested depending on the AWPA standard A7 method (AWPA 2012b). For extraction, the samples (around 1g) will be weighed into a microwave digestion tube and nitric acid will be added on to that. Digestion tubes will be sealed into digestion vessels and placed into a microwave oven for a pre-programmed digestion sequence. After the digestion was complete, each vessel will be quantitatively brought up to 100 ml volume with DI-water. The digested samples will be analyzed using a Perkin Elmer Optima 2100 DV Inductively Coupled Plasma Spectrometer (ICP)-OES to determine percentage Cu release after bioremediation. The percent reduction of Cu in the treated ground wood will be calculated based on the initial amount of Cu in the ground wood before
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