Culture And Sensitivity Of Bacteria

The purpose of the Unknown Project is to help microbiology students learn to identify an unknown bacterium in a mixed culture by performing different types of tests in a lab. The student must be familiar with the tests and should be able to analyze and interpret the results. Based on the results attained, an organism may be proposed. But what is a mixed culture? A mixed culture contains either two or more different types of identified microorganisms in a container, like a test tube for example. The culture may carry either organisms from the same bacterial family or it may contain a mixture of fungi and bacteria or other combinations. Being able to identify different types of organisms is essential for us to know if by any case, risks are presented. These cultures are performed mainly in hospitals. They are obtained from different parts of a patient’s body, depending on the suspected infection. Registered Nurses can perform certain cultures like blood, urine, sputum, etc. and send them to the lab. A mixed culture has been helpful in previous medical research by helping determine whether a patient has a bacterial infection or not. For example, “Sputum samples often give mixed cultures. Since the patient coughs up the sputum from the lungs, the sample can become contaminated by normal mouth bacteria, resulting in multiple types of bacteria in the culture.” (http://www.ask.com/science/mixed-culture-microbiology-af200d033ce00db0) Many tests may be performed on this mixed

Being able to figure out an unknown culture or bacteria is very important and a great knowledge to have. It helps people every day from finding cures to bacterial infections, discovering new kinds or simply just knowing the limits of what they are capable of. It allows scientist to know how to kill them treat then and ect. along with determining if they are harmful or benefit humans, and plants Along with being able to identify different species of

While stewardship is extremely important to slow the rate of resistance, we must also make sure that patients in need of antimicrobial therapy receive the treatment they need. Price of medication and overly cautious prescribers are two of many barriers that patients may experience; as clinicians it’s important that we do our part to promote appropriate and affordable treatments. Ideally, prescribers could determine the exact pathogen using a swab or culture of the infected tissue. This would prevent misdiagnoses and reduce unnecessary costs to the

Culture includes everything that a society is made of. There are many things that make up culture to include the economy, history, language, government and religion of a country. Those are some of the basics of defining characteristics of culture. For example the children of North Korea are trained from an early age to hate Americans. This kind of culture is

Physicians perform gram stains before prescribing an antibiotic to know if the cell wall type and the antibiotic sensitivity link.

Sample number 32 displayed beta hemolysis on the blood agar plate it was cultured on. I proceeded to gram stain the sample on a slide, and observed it at 1000x magnification. The organism is rod shaped, with no particular arrangement, and is gram negative. The appropriate identifying tests for this type of bacteria were performed, including the use of SIM agar, Urease, Citrate and OF Glucose tests. Once inoculated, these tests are incubated at 37 degrees Celsius for 24-48 hours. The findings of these tests revealed that the organism is motile, positive for indole production, negative for sulfur, negative for urease, negative for citrate and is a fermenter. The characteristics displayed are indicative of Escherichia coli.

One of the most important problems and lack of appropriate and timely testing and microorganisms grown in culture medium and prescribe the right medicine is based on the antibiogram.

During the waiting period the patient is given cocktails of drugs in hopes that one will be the proper medication and will kill their infection. This leads to overmedication of patients and the potential risk of antimicrobial resistant bacterial strains, for example: penicillin. Current methods require the culture of bacteria to be incubated and identified at specialized labs. Other faster methods of bacterial identification are in the works; however, all current methods require the identification of the bacterium and the care provider to prescribe and administer the appropriate drug. Nowhere in these tests is a drug administered to a sample of the patient to verify whether or not a resistant strain is present. Thus the patient is given a cocktail of drugs pre-identification and possibly the correct prescription a few days later with no guarantee of the drug’s effectiveness for the individual

Ensure correct treatment: Make sure that patients are being given appropriate antibiotic treatment. Also it is very important to make sure that there are enough antibiotics.

Aseptic Technique and Culturing Microbes(1) experiment was followed as stated in the lab manual from Clinical Microbiology Class C-453. Aseptic technique was initiated at the beginning of each experiment. Starting by cleansing the work surface with disinfected wipes to prevent cross contamination each time. Utilizing the gloves and personal protective equipment assisted in maintaining a pure culture during the series of experiments. The first step, was to grow the yeast and bacteria cultures. The materials used for the Aseptic Technique Experiment(1) were: test tube rack, 5 mL nutrient broth tubes, pipettes, a lit tea light, Fleischmen’s rapid yeast pack, E. Coli tablet and the S. epidermidis tablet. Started by activating the yeast, by placing the content of Fleischmen’s yeast bag in a disposable cup. Then added approximately 60 mL of warm tap water into the disposable cup. To assist in mixing the two items, I carefully swirled the cup. Once the mixture was combined, it then was left alone for around 10 minutes or until it was frothy. Each nutrient broth tube was labeled with the correct name of the microorganism that was cultured in that tube and dated. The S. epidermidis was selected first, to be cultured. Taking one of the 5mL nutrient broth tube in one hand, the pipette in other hand. The cap was removed with the same hand that had the pipette. Utilizing a lit tea light, like a bunsen burner, I flamed the mouth of the nutrient

Bacteria of the Bacillus genus are known to be rod shaped and are usually motile. They are endospore-forming and obligate aerobes, which means that these cells need oxygen to live. Bacillus are Gram-variable. They all begin as Gram positive; however, after time and aging of these cells, there is a decrease in peptidoglycan which is why they are considered gram variable. These cells can be arranged in a variety of different ways, including chains, pairs, and singles. The Bacillus genus consists of approximately 370 different species (Priest et al., 1988). However, of all those 370 species, the only two that are pathogenic to humans are Bacillus anthracis and Bacillus cereus. Not only does the Bacillus genus affect humans, but there are some

Bacterial identification procedures are extremely important in the clinical laboratory. Microorganisms need to be classified, and identified in order to distinguish one species from another. Identification can isolate organisms that are disease agents, and enable physicians to make diagnosis's. It also enables physicians to choose the best treatment/antibiotics to address that specific organism. For example, some gram negative bacteria can produce endotoxins when they die, which would be of concern to a doctor prescribing antibiotics. Identifying a specific bacteria as a source of a disease is the first step in moving towards discovering how it can be transmitted and preventing reoccurrence.

In science, media refers to any solid or liquid that is used as a platform for microbiological growth. There are numerous types of media that are used regularly in microbiology, each with a different makeup, making each type of media unique. Whether nutrient agar and broths, minimal agar, chemically specific agar made of a combination of certain chemicals or chemically undefined agar which is made up of composites of different materials, the media used for growing and observing microorganisms is important and within each test, experiment, or observation, the precise media used is specifically chosen.

The first class are Enzyme Linked Immunosorbent Assays (ELISA). ELISAs detect antigens produced by reactions between antibiotics and cells in the body. Antigens are stuck onto a plastic

Microbial cultures are foundational and basic diagnostic methods used extensively as a in research tool in molecular It is important to have pure culture isolation. A pure culture is a population of cells as a multicellular organisms growing in the absence of other species or types.