Curcuma Longa Essay

The preparation for the experiment started by gathering the solutions of enzyme Peroxidase, substrate hydrogen peroxide, the indicator guaiacol and distilled water. Two small spectrometer tubes and three large test tubes with numbered labels. In addition, one test tube rack, one pipet pump and a box of kimwipes were also gathered. Before the experiment, the spectrometer must be set up to use by flipping the power switch to on. Following, the machine was warmed up for 10 minutes and the filter lever was moved to the left. In addition, I set the wavelength to 500 nm with the wavelength control knob. Before the experiment, I had to create the blank solution by pipetting 0.1 ml of guaiacol, 1.0 ml of turnip extract and 8.9 ml water into tube #1. Following the creation of the blank, a control 2% solution was created.

From this graph and chart we can see that the higher the concentration the higher the absorbance, all the different concentrations were tested at the same wavelength (625nm). Also we can determine our unknown substances concentration by using the absorbance we got for it. The red dot on the graph followed by the line towards the horizontal axis indicates that the concentration of fast green was 34% or 5.1x10-3.

After this, the solution was poured into a volumetric flask just about to the 1dm3 line and then it was left there to cool to the same temperature as the room before filling precisely to the 1dm3 line with distilled water. The molar mass of CuSO4.5H20 was 249.5 so that means 249.5g of copper sulphate was needed to dissolve, in order to make a standard solution, into 1dm3of distilled water. Following this, a linear dilution of the CuSO4.5H2O was made in order to be used to make a calibration curve after using the colorimeter to write down the absorbance of each sample. A linear dilution is diluted with distilled water in order for it to make the concentration weaker and weaker. For this investigation, the dilutions made ranged from 0.01 to 0.1 M/l . It was essential to only make up 10cm3

10 microliters of the sample is then added and the assay absorption is measured at 340nm. If absorbance was above 1.5, samples were diluted.

Patient safety and hospital acquired infections (HAI) are deemed highly important in the health care setting. My organization uses quality indicators pulled from EPIC, which is our health information system, to ensure we are meeting regulations for catheter associated urinary tract infections (CAUTI). Data includes rates of infections, length of foley catheter placement, reasons for foley placement, as well as facility specific documentation that is used to aide in the prevention of CAUTI. By pulling this data, one could identify trends affecting rate of infections. This may lead to a change in policy or procedure that can improve the rate of infections for those patients with foley catheters. Thus decreasing the percentage of HAI’s for

The purpose of this experiment is to prepare Allura Red standard solutions, use a colorimeter to measure the absorbance value of each standard solution, find the relationship between absorbance and concentration of a solution and use the results of this experiment to determine the concentration of Allura Red

Make sure to use the same type of cuvette to keep the width consistent and to prevent any experimental error from arising. Obtain 5 of the same type of cuvettes and pre-rinse them thoroughly. Label them numbers one through five in increasing molarity. Then, fill each of the cuvettes with one of the five solutions you created back in Part A. We will first examine the solution that exhibits the highest concentration or molarity. Make sure to wipe the outside of the cuvette with a Kimwipe before placing into the SpectroVis Plus device. Observe the graph that is generated and make sure to take note where the maximum absorbance takes place.

Whenever I am asked where I am from I always respond, “South Texas”. People will reply with a question, San Antonio? , and I usually have to clarify that I am south of San Antonio, about four hours away no San Antonio is not south texas, the Rio Grande Valley is south texas. In the Rio Grande Valley there is a town named Weslaco around five minutes from the border of Mexico. We live in a community swarming with Mexican culture. Our city’s population is approximately 36,000 and from this population about 85 percent is composed of Hispanics or Latinos. As if we are our own country we have our own language which consists of Spanish, English, and Spanglish. We are knee deep in culture so it is easy to be against the norms in our community, and personally I have dealt with this problem.

It has been rumored that are extraordinary beings on another familiar planet close by our home of Nacirema, and it is our goal as the Interplanetary Nacirema Research Center team to investigate the situation. We plan to focus our trip on the specific coordinates that the University of Connecticut campus lies on, and being the curious creatures we are, we plan to delve into specific areas within this concentrated area. We will be studying the way both males and females conduct their everyday lives in terms of living, learning and communicating. Through our research, we plan to lighten up the mysteries about this foreign species and enable ourselves to communicate

Where A is the initial absorbance when the experiment first starts, l is the path length of the cuvette (2.54 cm), and [CV]t is the initial concentration of crystal violet.

On the other hand Nowrasteh defended that the E.U. problem is an issue of segregating refugees. Immigrants are unlikely to find employment and in some countries like Germany they are not even allowed to travel outside their assigned geographical area. This limitation makes it harder to find jobs. When it comes to the U.S., Nowrasteh adds, the government is incapable of enforcing sound immigration policy and the best way to deal with the issue is to encourage legal immigration. According to Nowrasteh, research has shown that the demand to come to the U.S. unlawfully decreases when the country encourages legal immigration. He concludes “politicians should have little to do with decision making in immigration if we need to resolve the issue.”

Illiana Cardona,105, of Blackfoot, Idaho, recently passed away on November 24, 2105. She passed peacefully in her sleep after finishing her extensive research essay that held the answer to cure all known and unknown diseases. “She was a real fighter as well as stubborn,” Avenir “Ave” Mari told reporters after announcing his wife’s death,” It took me years to convince her to marry me.”

Incorporation of assay controls included setting up a spectrophotomer and running the chart recorder with a full-scale deflection before the start of the assay. The set recorder had a corresponding value of 1 for the change in the absorbance. Therefore, prior testing was done to observe whether a change occurred in the readings. This helped to indicate that the results were valid, as they could have been affected by a fault during the setting up of the spectrophotometer. On the other hand this was considered as one of the controls for the experiment. Nevertheless, a new cuvette had to be used for each assay.

After wearing the gloves we obtained a chromatography vial from professor and label it with my and my peer initials. We dried up the chromatography vial in fume hood and added 1 ml of chromatography solvent to the vial. Then we took a chromatography strip and measure it 1.5 cm with ruler from one end of the strip and drew a line with pencil we cut two small pieces below the pencil line to form a pointed end. We applied spinach on the strip using quarter to rub the spinach leaf on the line that we drew on the strip and put it into the chromatography vial and placed that in fume hood. We observed as the solvent was moving up the chromatography strip by capillary action. When the solvent was reached approximately 1 cm from the top of the strip then we removed the cap from the vial. We took out the strip from the vial using forceps and marked up the location of the solvent front because it evaporates quickly. We measure out the distance as well as the pigment in order to find out the rf value. Moreover we compared rf values to the one in reference list in order to identify the

Remove foam plug from the inoculate flask and pour it into a cuvette. Incubate it at 37°C and the optical density readings at 600nm every 10-15 mins. Pour into the