DETERMINING THE GLUCOSE CONTENT OF AND ORANGE USING GOD Essay

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DETERMINING THE GLUCOSE CONTENT OF AND ORANGE USING GOD-PAP ASSAY Every fruit has a sweet taste only that some are sweeter than others. The sweetness of most fruits come from its sugar content and these sugars that the fruits contain are known as invert sugars. In this experiment, an orange was used. An orange which is an excellent source of vitamin C gets its sweetness from natural sugars which are sucrose, glucose and fructose (livestrong.com). In this experiment the concentration of glucose in an orange was measured. There are various methods which can be used to measure the glucose concentration but in this experiment a highly specific enzymatic method using the GOD-PAP assay (glucose oxidase peroxidase aminophenazone phenol)…show more content…
The absorbance values shown in the table have just a slight difference from one another which shows that the values were accurate. These values were taken using a spectrophotometry skills and a spectrophotometer. A spectrophotometer is a device that shines light through a solution measuring its absorbance of the light at certain wavelengths. In this experiment, the absorbance of standard and diluted glucose solutions were taken at 500nm. These absorbance values as shown in table 1 were used to plot a calibration curve to find an unknown concentration of glucose. From the table there were duplicate values for each solution or sample. These samples were then plotted on a graph and a line of best fit was drawn through those points. To obtain the concentration of glucose based on the graph ,the value of one of the unknowns was chosen which was within the range of the graph was chosen and traced to meet the line of best fit and the concentration was derived. From the table, absorbance increased as the concentration increased showing precision and as for accuracy, the difference between the first and second trials were minimal. In this experiment and in other experiments like these, errors could be caused by improper handling of GOD-PAP assay and cuvettes .incorrect placement of cuvette into the spectrophotometer and poor calibration of the spectrophotometer. Errors could also be made when plotting a graph for the calibration

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