DNA Origami Techniques

1543 WordsFeb 24, 20186 Pages
Strands of DNA may either be twisted or curved into Nanoscale Shapes through folding. Multiple combined curved elements may be formed in order to build up many different types of complex nanostructures like square-toothed gears and wireframe beach balls. Complex higher-order structures to form multiple double-helical segments which are connected through a lot of turn regions can be engineered by the sequence of molecules of DNA. Shapes of nanoscale of the 1- to 100-nm (1-4) have been used in constructing two-dimensional (2D) crystals (5), 3D wireframe polyhedral (12-17) and nano tubes (6¬-11). A very powerful method to direct the self-assembly of the custom-shaped, planar arrays of anti parallel helices and megadalton-scale of the multiple kilo base “scaffold strand” has been introduced. In this case, a single staple strand can pair with many scaffold-strand segments in order to achieve the intended strategy of switching. With a strategy of extended DNA origami to 3D nano construction it can be conceptualized as anti parallel helices (19) by stacking corrugated sheets. In order to produce a variety of 3D shapes, the arrangement, individual lengths and number of helices may be tuned so as to help the design process (20) through graphical software development. By doing this, the design spaces of accessible DNA-origami shapes are expanded in order to increase the diversity of nanostructures curvature and designed twists in relation to nanoscale. The numbers of base pairs in
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