Daphnia Heart Rate Experiment Essay

According to the results, the columns of caffeine in figure 1, of this experiment the hypothesis for caffeine is partially accepted. There is an increasing trend in the change of pulsation rates with increasing

What do you think would happen if the two lungs were in a single large cavity instead of separate cavities?

After completing the experiment we found that when we gave the Daphnia caffeine the heartbeat rate did show an increase. However, we also found that alcohol also increased the number of times the heart beat. Even though we performed all of the experiments very carefully, we cannot be certain that the effect we saw was due to the drugs. Perhaps the change in heartbeat rate is caused by

After the Daphnia was given time to calm down, the team took a reading of its heart rate at room temperature (27 degrees C). The reading was taken by counting the heart beats for ten seconds and then multiplying by six to yield beats per minute. Next, a glass Petri dish was filled with ice water at five degrees Celsius. The cold water Petri dish was placed on the stage of the microscope, and the Daphnia was placed on top of the dish. When the Daphnia had been given a minute to acclimate to the changes, another heart rate reading was taken. Then the same procedure using the Petri dish to changed environmental conditions was used with cold tap water (23 degrees), warm tap water (30 degrees), and hot tap water (45 degrees). A heart rate reading was taken for each temperature.

Resting heart contractions were recorded for thirty seconds until the heart rate was less than 60 beats per minute. A stimulator electrode to be used was set to the following states: Amplitude of 4.00 Volts, a stimulus delay of 50ms, stimulus duration of 10ms, a frequency of 1.0Hz, and a pulse number of 30. The electrode was then placed in direct contact with the heart for 30 seconds at which time the data was observed and recorded.

In Exercise 1, diaminofluorene is used to determine the hemoglobin concentration in the daphnids. A higher hemoglobin concentration is indicated by a darker blue color. A spectrophotometer was used to determine the absorbance at 610nm. When measuring the absorbance levels a blank is necessary to have a zero reference, the blank is the “starting point” for the measurements of the sample (re-word). The blank consists of 10µL of diaminofluorene(DAF), 50µL of hydrogen peroxide, and 0.5mL of PBS. The PBS acts as a buffer in this experiment. The cuvette with the sample of daphnids consisted of 10µL of DAF, 50µL of hydrogen peroxide, and 0.5mL of the sample of Daphnia. In Exercise 2, the Pasteur pipette was used to obtain the sample of Daphnia. The depression slide used in this experiment isolated the daphnid, cotton was used to keep the daphnid still while the heartbeat was counted. The ocular micrometer on the microscope allows the tail spine length to be measured accurately, as well as using an ocular magnification table.

It is important to check the placement of the tube before suctioning or feeding, since

Ecosystems have a way of maintaining themselves under ordinary conditions but when a new factor is added that will most likely threaten the environment action needs to be put forth. Our environment is affected by many pollutants on a daily bases forming a threat to natural stability. For example some of the most polluted parts of the environment are bodies of water like that explained in our lab. Once pollution occurs animals can be affected in many ways even to the brink of death. In the lab the stream is getting pumped with salty potassium filled water that can kill daphnia, habitants of the stream, if the concentration gets too high. To prevent this a

The effects of caffeine and alcohol on daphnia are expressive of whether these substances are harmful or beneficial to the organism. By understanding the results of this experiment, it may also be understood how these substances effect humans. In this study, one daphnia was exposed to increasing levels of alcohol, while the other was exposed to increasing levels of caffeine, each in order to test the hypothesis that when given amounts of caffeine and alcohol, the daphnia will be affected the same way a human would. The effect of each substance was measured by the daphnia’s heart rate one minute after the substance was added. Results reveal that alcohol slows the heart rate, while caffeine increases heart rate. Furthermore, caffeine shows a

The purpose of this lab is to test what effect of Nicotine, Acetylcholine, Epinephrine, Caffeine, and Ethanol on an organism’s heart rate.

In this experiment we find how caffeine can affect the heart rate of a culture Daphnia. Heart rate of a living organism’s can vary depending on the individual, age, body size, heart conditions, medication use and even temperature. This report will examine if the caffeine is good or bad for the living organism’s health and body. And discuss about where the caffeine is produced and used in daily life of human beings and on the environment. Daphnia is a water flea used in this experiment because of its genomic infrastructure with wide range of phenotypic diversity. This quality of Daphnia makes them a versatile model for the experiment. Also their transparent body allows the experimenter to visually see how the heart beats and count them under the light microscope during the experiment as required. The heart rate of Daphnia is monitored under different concentration of caffeine solution and the results are shown in a table and a graph. Experiment carried out to locate the effects of caffeine on a heart rate of Daphnia may or may not be a predictor of change in human heart rate under caffeine. The effects of caffeine can also be tested on humans but those experiment involving humans contains high risk, as Daphnia can only live for a short period of time and in nature most of them get eaten within their first few days or weeks of life.

My hypothesis was, as the concentration of alcohol increases the lower the heartbeat of the Daphnia will fall. To test my prediction I carried out 25 individual experiments which were all carried out using the same procedure but varying the alcohol concentration of the solution. To investigate how alcohol concentration affects the heartbeat of Daphnia I recorded the heartbeat of 25 Daphnia for a period of 7 minutes. This included 1 minute before submersion in alcohol, 3 minutes during submersion in alcohol and 3 minutes after submersion in alcohol. I then found the percentage decrease of the heartbeat of the Daphnia from the resting heart rate.

This experiment is being performed to show the effect of pH levels on daphnia by changing pH levels and measuring the heart rate.

When migration occurs the filter shifts its position, and thus become ineffective at preventing a pulmonary embolism. It may in a more serious move, perforate the inferior vena cava or shift into a position where the device is impossible to remove.

The heart is one of the most important organs in an organism’s body, no matter if they are aquatic, amphibian, or a mammal. This super organ works automatically, able to pump massive amounts of oxygen rich blood through the body by means of electrical impulses and the opening and closing of valves within its many layers. It is what keeps us and every other creature on this earth alive; so it is only natural for one to fear when there might be a problem with one’s heart. A cardiac arrhythmia can happen to anyone, no matter the age, race, or gender, and as such, doctors and scientist have spent years trying to better understand the heart and the way it functions so that they can try to prevent these problems and save millions of lives.