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Daphnia Lab Essay

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Daphnia and Additives Lab
Purpose
The purpose of this lab is to test what effect of Nicotine, Acetylcholine, Epinephrine, Caffeine, and Ethanol on an organism’s heart rate.
Materials
* Microscope * Eye dropper * Pipet * Cotton balls * Depression slide * Beakers * Daphnia * Water * Five additives: Nicotine, Acetylcholine, Epinephrine, Caffeine, Ethanol
Procedure
1. Put on safety goggles. 2. Catch a living Daphnia. 3. Transfer to a depression slide and add water. 4. Add cotton ball fibers to restrict movement of the Daphnia. 5. Establish the resting heart rate of the Daphnia. Count the amount of beats for ten seconds then multiply that number by six to convert it to beats/min. …show more content…

A slowed heart rate and the associated fall in the rate of expulsion of blood from the heart is sufficient to maintain body function during rest, and conserves energy in the heart under conditions where its supply (and the supply of oxygen in the blood) are diminished. A drug that slows heart rate is called a negative chronotrope.
Epinephrine/adrenaline: Heart rate is increased by the sympathetic nervous system and the hormone adrenaline circulating in the blood via activation of cell surface receptors in

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