Daphnia Lab Report

The purpose of this experiment was to determine the relationship between tail spine length and hemoglobin levels as well as the relationship between tail spine length and heart rate. The concentration of the hemoglobin in Daphnia is dependent on the oxygen available to them.

Daphnia, Branchiopoda, are categorized under the phylum Arthropoda and subphylum Crustacea. Daphnia are planktonic and are characterized by flattened leaf-like legs used to produce a water current for the filtering apparatus (Ebert et al. 2005). Daphnia are suspension feeders, which is one reason why they are used in this experiment. The purpose of this experiment is to see how Nyquil® and ethanol affect Daphnia heart rate. Nyquil® is a pharmaceutical drug that is used to treat common cold, aches, or pains, and the main ingredient is acetaminophen. Acetaminophen is a pain reliever that, like other antibiotics, inhibits protein synthesis, and has proven to immobilize Daphnia in even low concentrations (Daughton et al. 1999). There are many reasons why we use Daphnia in science; one reason is that the Daphnia hear rate variation test can be used in evaluating the effect of xenobiotics and in selecting agents for the pharmacological correction of this functional parameter (Dolgo-Saburov et al. 2008). We predict that Nyquil® decrease the heart rate while ethanol will increase it.

The purpose of the experiment was to examine the effects of certain drugs, like alcohol or caffeine, on the heart of a Daphnia magna. From what is known about our hearts, I hypothesized that the Daphnia magna would have a stimulant reaction to both alcohol and caffeine. To begin the experiment we had to prepare the organism to have a clear reading and obtain a control group. Once she was ready, we applied the drug to the organism and counted its heart beat for ten seconds, we did this procedure a total of three times for both caffeine and alcohol. As a result, the Daphnia magna caffeine treatment heart rate is considerably higher than the control heart rate and lower than the alcohol treatment.

The physiological effect of different medicines is successfully determined by counting the heart rate of Daphnia through this experiment. The data shows that different chemicals affect the heart rate of Daphnia differently. In the presence of Ephedrine, the heart rate of Daphnia has increased, and in the presence of Ethyl Alcohol and caffeine, the heart rate has decreased. The Daphnia under water is a control group, and the average heart rate is 269 BPM. The heart rate has decreased to 267.33 BPM when 0.5M Ethyl Alcohol is added and to 256 BPM when 0.5M caffeine is added.

The experiment goal was to measure the effects that different water temperatures had on the Daphnia's heart rate and the effects of epinephrine as well. Daphnia is commonly known as water flea bugs that are small aquatic crustaceans that is found plentifully in small bodies of fresh water (General Biology Study Guide and Lab Manual, 10th ed. 2007). These water bugs are good animals to use for this type of experiments because they are large enough to be seen without a microscope in light and under the microscope at twenty times magnification their internal organs can be seen straight through their body cavity. Daphnia are ectothermic creatures, meaning

The purpose of this experiment was to test the affect of caffeine on the heart rate of Daphnia by observing their behavior and heart rate under a microscope when exposed to different concentrations of caffeine. Caffeine is a stimulant drug used in many energy drinks and causes large amounts of stimulatory neurotransmitters to be released. Therefore, as caffeine is a stimulant drug it was hypothesized that the Daphnia heart rate would increase immensely. Daphnia is a group of microscopic, planktonic crustaceans that are arthropods that measure 1-5 millimeters in length. Daphnia live in multiple aquatic environments such as freshwater lakes, ponds and rivers. “The development of genomic infrastructure coupled with a wide range of phenotypic diversity make Daphnia a versatile model

Studies of Daphnia presented information which elluded to the idea that Daphnia would react to environmental pollutants within a relatively short timeframe; therefore, a lab was constructed to find the effects of copper sulfate in a Daphnia’s system. Before beginning, the hypothesis was gathered: if the concentrations of copper sulfate in the water are high, then the Daphnia would be afflicted with symptoms associated with physical decline. Daphnia, known as water fleas, are small crustaceans who get their common name from their jerky movements. The organisms reside within lakes and ponds often in limestone-based areas found all over the world. Daphnia consume algae, specifically the free-living green type, yeasts, and bacteria; therefore,

Remember to use an efficient counting method and count the Daphnia’s heart rate for 15 seconds, multiply all your trials by four to get the beats per minute. Do no less than five trials to be accurate. Keep in mind that you need to test the Daphnia’s heart rate in its regular habitat before performing the

The second variable tested was hydrotaxis in sowbugs. To do this, we first removed the cardboard shade cover. We did this to remove the dark environment variable. This way the only variable being test is wet versus dry. We then opened the lid and placed drops of water on approximately half of the petri dish and placed the lid back on it. Then we recorded the number of sowbugs in both the wet and dry locations every minute for the next twelve minutes. As shown in Table 2, the sowbugs in our experiment were positively

Heart rate of daphnia fell from an average of 274.455+/-26 beats per minutes to an average 246.868+/-71 heart beats per minute. After performing Mann-Whitney U statistical test, this difference in heart rate was found to be statistically not significant.

After the Daphnia was given time to calm down, the team took a reading of its heart rate at room temperature (27 degrees C). The reading was taken by counting the heart beats for ten seconds and then multiplying by six to yield beats per minute. Next, a glass Petri dish was filled with ice water at five degrees Celsius. The cold water Petri dish was placed on the stage of the microscope, and the Daphnia was placed on top of the dish. When the Daphnia had been given a minute to acclimate to the changes, another heart rate reading was taken. Then the same procedure using the Petri dish to changed environmental conditions was used with cold tap water (23 degrees), warm tap water (30 degrees), and hot tap water (45 degrees). A heart rate reading was taken for each temperature.

This experiment is being performed to show the effect of pH levels on daphnia by changing pH levels and measuring the heart rate.

The mole is a convenient unit for analyzing chemical reactions. Avogadro’s number is equal to the mole. The mass of a mole of any compound or element is the mass in grams that corresponds to the molecular formula, also known as the atomic mass. In this experiment, you will observe the reaction of iron nails with a solution of copper (II) chloride and determine the number of moles involved in the reaction. You will determine the number of moles of copper produced in the reaction of iron and copper (II) chloride, determine the number of moles of iron used up in the reaction of iron and copper (II) chloride, determine the ratio of moles of iron to moles of copper, and determine the number of atoms and formula units involved in

Common testing conducted by researchers uses many features of Daphnia. The transparency of Daphnia and the visibility of their hearts is the basis for the majority of experiments conducted on Daphnia. (Villegas-Navarro, Roses-L & Reyes, 2003). Many other researchers have conducted experiments on Daphnia while also paying attention to the cardiovascular region of Daphnia (Campbell & Matthews, 2004). Researchers have

The experiment took place in a laboratory setting, and the first step was obtaining sixty individual Daphnia magna (that were neither adults nor tiny offspring) from a large tank in the lab. These individuals were equally divided into three groups; low density, medium density, and high density. The twenty Daphnia assigned to the low density group were split into four groups of five and pipetted into one of four tubes filled with 10mL of Chlamydomonas algae. The twenty Daphnia assigned to the medium density group were split into two groups of ten and placed into one of two tubes also filled up to 10mL with Chlamydomonas. The final twenty Daphnia were all placed into a single tube filled with 10mL of the algae. In order to avoid suffocation-related