Daphnia Lab Report Sample

This lab was about how a goldfish’s breathing rate changes in different temperatures in order to maintain homeostasis.

Three Daphnia magna were placed in a petri dish with a small amount of spring water. Each Daphnia magna was measured separately. The Daphnia magna was placed on a slide and their control heart rate was measured for 15 seconds under the microscope and multiplied by four. Then, it was placed in the epinephrine solution for two minutes.The Daphnia magna was placed back onto the slide with the spring water and the heart rate was measured again for 15 seconds and later multiplied by four to observe the effect of epinephrine on the heart rate. They were then placed into a different petri dish of spring water after their experimental heart rate was recorded. All the heart rate values were placed into a Microsoft Excel document. The controlled variables included the temperature of the water, the time subjected to the epinephrine solution, and the time used for measuring the control heart rate and the epinephrine heart

The experiment posed some small floors that most likely changed the results of the experiment. For example, by the time the 1% ethanol was added the daphnia would have already been feeling fatigue as it had just reacted to the 1% caffeine solution which increased its heart rate dramatically and it would also be feeling drowsy from the minimal amount of water it was living in providing it a very small amount of oxygen. To fix this issue I believe we could have added

effected the heartbeat rate of a Daphnia. The results of the experiment were that the

Ectothermic animals are animals whose body temperature is affected by their surroundings. This means that if the environment is cold the animal will be cold. If the environment is warm the animal will be warm. This is because the animal doesn’t have the capability of regulating its body systems to keep a constant body temperature. When an ectothermic animal is cold, its heart rate will lower. When the animal is warmer, the heart rate will raise – as long as the temperature isn’t sufficiently high to harm the animal. (Campbell, 2005)

In Exercise 1, diaminofluorene is used to determine the hemoglobin concentration in the daphnids. A higher hemoglobin concentration is indicated by a darker blue color. A spectrophotometer was used to determine the absorbance at 610nm. When measuring the absorbance levels a blank is necessary to have a zero reference, the blank is the “starting point” for the measurements of the sample (re-word). The blank consists of 10µL of diaminofluorene(DAF), 50µL of hydrogen peroxide, and 0.5mL of PBS. The PBS acts as a buffer in this experiment. The cuvette with the sample of daphnids consisted of 10µL of DAF, 50µL of hydrogen peroxide, and 0.5mL of the sample of Daphnia. In Exercise 2, the Pasteur pipette was used to obtain the sample of Daphnia. The depression slide used in this experiment isolated the daphnid, cotton was used to keep the daphnid still while the heartbeat was counted. The ocular micrometer on the microscope allows the tail spine length to be measured accurately, as well as using an ocular magnification table.

In order to complete this exercise, two different procedures were conducted; one measured the basal heart rate, and the other measured the drug-induced heart rate. A sample 's basal heart rate can be defined as the “resting” heart rate. This is when no drugs or altering substances are applied. In comparison, the drug-induced heart rate of a sample can be defined as the heart rate after the drug was administered to the surrounding environment. The four drugs tested on the Daphnia specimens were Acetylcholine, Caffeine,

The effects of caffeine and alcohol on daphnia are expressive of whether these substances are harmful or beneficial to the organism. By understanding the results of this experiment, it may also be understood how these substances effect humans. In this study, one daphnia was exposed to increasing levels of alcohol, while the other was exposed to increasing levels of caffeine, each in order to test the hypothesis that when given amounts of caffeine and alcohol, the daphnia will be affected the same way a human would. The effect of each substance was measured by the daphnia’s heart rate one minute after the substance was added. Results reveal that alcohol slows the heart rate, while caffeine increases heart rate. Furthermore, caffeine shows a

Caffeine was chosen because it raises the heartbeat of humans, so they wanted to see what would happen when given to a Daphnia. One drop of Caffeine was dropped onto the Daphnia and then observed to get a recording of the heart rate. The results for both of the solutions were different which arose the question of whether the Caffeine had an effect on the Daphnia’s. The control group was tested first with 5 trials of different Daphnia, this gave an average heart rate of 114.8 with a difference of 0 between the two Daphnia. The heart rates were recorded 3 times for each Daphnia, for each trial 2 Daphnia’s were tested. When the Daphnia were placed in the toxin the results showed a very obvious change in heart rate with an average of 15.6 in heart rates between the Daphnia placed in water and the Daphnia mixed with caffeine. The experiment was done in a controlled environment in the Parker build at

The purpose of this experiment was to see if caffeine had an effect on the heart rate of Daphnia (water fleas) by observing them under the microscope and recording their heart rate over a period of time in concentrations of caffeine. Caffeine, which is a stimulant drug rttused in coffee, tea, Red Bull and Pro-plus, causes increased amounts of stimulatory neurotransmitters to be released. As caffeine is a stimulant, a prediction was made that caffeine would increase the heart rate of the Daphnia.

In this experiment there were many possible errors. One of the big errors that was made was that instead of using the plastic water holder to hold the warm water, the experiment was done by putting a wet room temperature paper in one chamber and a another paper dipped in warm water for the other chamber. The data was recorded half way through the experiment but unfortunately it isn't present as of now. Another error that was present was that when the time was started, the isopods were interrupted with a paint brush trying to move them. That could have slightly skewed the start of the data. One other error could have been not paying attention to the time and counting the isopods at different time

Another factor that may decrease the validity of the results is that the heart rate was only counted for 20 seconds at a time and then multiplied by 3 to calculate beats per minute. This was done for ethical reasons – so that the daphnia could be placed back in it’s natural habitat as quickly as possible. This could have devaluated the

In this experiment we find how caffeine can affect the heart rate of a culture Daphnia. Heart rate of a living organism’s can vary depending on the individual, age, body size, heart conditions, medication use and even temperature. This report will examine if the caffeine is good or bad for the living organism’s health and body. And discuss about where the caffeine is produced and used in daily life of human beings and on the environment. Daphnia is a water flea used in this experiment because of its genomic infrastructure with wide range of phenotypic diversity. This quality of Daphnia makes them a versatile model for the experiment. Also their transparent body allows the experimenter to visually see how the heart beats and count them under the light microscope during the experiment as required. The heart rate of Daphnia is monitored under different concentration of caffeine solution and the results are shown in a table and a graph. Experiment carried out to locate the effects of caffeine on a heart rate of Daphnia may or may not be a predictor of change in human heart rate under caffeine. The effects of caffeine can also be tested on humans but those experiment involving humans contains high risk, as Daphnia can only live for a short period of time and in nature most of them get eaten within their first few days or weeks of life.

My hypothesis was, as the concentration of alcohol increases the lower the heartbeat of the Daphnia will fall. To test my prediction I carried out 25 individual experiments which were all carried out using the same procedure but varying the alcohol concentration of the solution. To investigate how alcohol concentration affects the heartbeat of Daphnia I recorded the heartbeat of 25 Daphnia for a period of 7 minutes. This included 1 minute before submersion in alcohol, 3 minutes during submersion in alcohol and 3 minutes after submersion in alcohol. I then found the percentage decrease of the heartbeat of the Daphnia from the resting heart rate.

The objective of this investigation was to observe the affect of caffeine on the heart rate of a Daphnia (water flea), by observing the specimen under a microscope for a set period of time while in an environment of concentrated caffeine. By using a microscope, the collection of data regarding the heart rate of the how to make a line graph in word Daphnia was possible. Drinks such as coffee, Red bull, MONSTER and Coca-Cola contain high amounts of caffeine, causing the consumer to increase their amounts of stimulatory neurotransmitters (this is a chemical in the brain sent throughout the body telling your heart to beat, your lungs to breathe, and your stomach to digest). As caffeine is a stimulant, it is predicted that the drug will increase the Heart Rate of the Daphnia. Caffeine will be used in this experiment with concentrates of black coffee.