Dcpip Testr

At five minute intervals over the next fifteen minute period, record the color intensity of the solution of each test tube.

Step 4: Place the test tubes into separate coffee cups to maintain the upward position. Add 2 mls of the catalase solution to each of the test tubes and then place tubes1, 2, and 3 in the conditions described above. For test tube 4, fill the coffee mug half full of boiling

4) One package of active dry yeast was added to bottle labeled ‘5mL’ and solution was swirled.

After 30 seconds the same enzyme volume was added to the second flask. This was continued to the third, fourth and fifth flasks with interval of 30 seconds until the final addition occurred at 2.5 minutes.

12.Stir then pour 2.5ml of the enzyme mixture into one of the test tubes not allowing any water from the saucepan into the test tube.

The first step that needed to be done in this experiment was adding hydrochloric acid (HCl)

24) Separate the formed crystals (benzoin) and allow them to dry, then take the MP.

9. Each of the other solutions is already approximately 0.1 M. With these solutions you can pour a small

3. We poured tube 1 with the solution in tube 3 to combine them. We repeated this for all of the tubes. Each of the tubes in step 1 was mixed with a tube in step 3, making there be 6 total test tubes with a solution in it.

Fill a test tube about 1/3 full with cold tap water for use in step 34.

Again, label 7 1.5ml tubes 0 thru 6. Place 15μl of each serially diluted extract into its corresponding labeled tube. Next add 465μl of media into each tube. Then 60μl of Alamar blue in each tube. Finally add an additional 60μl of cells (adjusted to 10,000 cells/20 μl). Vortex each tube for 5 seconds. Now, take 3 different samples 190μl samples of concentration 0 and put it in Wells A2, B2, and C2. Repeat this step again by taking 3 more different 190μl samples of concentration 1 and putting it in wells A3, B3, C3. It should be noted that it is important to vortex each 1.5μl tube again be-fore putting it into the 96 well plate. Contin-ue this same procedure consecutively for the re-maining concentrations.

1.) Transfer the distillate to separatory funnel. Fluid didn’t seem very clear but sufficient to finish our lab on time.

In this experiment I will be finding out which fruit juice contains the most vitamin C. I will be using a method similar to titration but I will be using a syringe instead of a burette.

Then, each group of students received the necessary materials to complete the experiment. When the students received the cups, they labeled cups to distinguish between the salt solution, distilled water, and control group. After weighing the cups and finding the mass of the cucumbers, the students poured 50 ml of water in one cup, 50 ml of salt solution in the other, and left the control cup empty. Then, the students placed the cucumbers into the cups and waited 30 minutes for the results. After the 30 minutes, the students removed the cucumbers from each solution and dried the cucumbers with paper towels. The students then weighed the cucumbers again and recorded their results. Lastly, the students found the difference from the original mass of the cucumbers and recorded their results.

Orange Juice Materials: Three oranges were used for this experiment to obtain a decent amount of juice. Each orange was cut in half and juiced according to steps 1 and 2 of Appendix I of the Laboratory Manual for Biology 201 (Denniston, Wimmers, & Hemm 2015). The juice from the first orange and one half of the second orange was strained into a small beaker following