Description of Agrobacterium Tumefaciens Essay

594 WordsApr 29, 20083 Pages
INTRODUCTION Agrobacterium Tumefaciens is an aerobic gram negative bacteria that is commonly known as a causal agent of Crown Gall disease in a wide array of plants. Agrobacterium Tumefaciens uses horizontal gene transfer “which is any process in which an organism transfers genetic material to another cell that is not its offspring.” (Black, Microbiology, pg13) to cause tumors in plants. It causes tumors by transferring a small segment of its DNA and inserting itself in a semi random location in the plants genome. We investigated the effect Agrobacterium Tumefaciens had on the Kalanchoe plant and how it supported Koch’s Postulates. Koch’s Postulates was designed to determine the causal agents of plant disease and to associate a…show more content…
DISCUSSIONS The purpose of this experiment was to investigate Agrobacterium Tumefaciens effect on the Kalanchoe plant and how it supported Koch’s Postulates. “Koch’s postulate state that inoculation of a sample of the culture into a healthy susceptible animal or plant must produce the same disease. “ (Black, Microbiology, pg13) The results that I obtained in this experiment support this, that DNA from the Agrobacterium Tumefaciens had transferred itself in a semi random location in the plants genome which yielded a white tumor growth on the leaf. Also Koch’s Postulate states that “The specific causative agent must be found in every case of the disease.” (Black, Microbiology, pg13) Agrobacterium Tumefaciens is commonly known as causal agent of Crown Gall disease amongst plants. Koch’s third postulate states that “the disease organism must be isolated in a pure culture.” (Black, Microbiology, pg13) This is also proven, as the original bacteria that I used to inoculate the plant with was isolated in a pure culture. Additional experiments that can be conducted, could be conducting the experiment again but this time rather use a wider variety of plants, instead of just one plant why not use several different plants. Other options could be that we conduct the same experiment only this time increase the

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