Design Experiment

When placing the large test tube in boiling water, be sure to keep the face of the large test tube away from you or any lab partners with safety tongs.

2. Used a test tube clamp to held the test tube and gently heated the tube in a laboratory burner flame for about 30 seconds.

We first started this experiment by obtaining twelve 15ml test tubes, in which we placed in a rack and labeled each with what

3. We poured tube 1 with the solution in tube 3 to combine them. We repeated this for all of the tubes. Each of the tubes in step 1 was mixed with a tube in step 3, making there be 6 total test tubes with a solution in it.

To ensure the experiment was kept as a fair test a number of variables were controlled. The temperature of the solutions was kept constant by placing the boiling tubes into a test tube rack and setting it into a water bath with a fixed temperature of 25oC. The temperature needed to be kept low and fixed as a high temperature would denature the enzymes, they would therefore be unable to break down the gelatine and no results would be produced from the experiment. Keeping a constant temperature also meant that the solutions reacted at the same rate.

Based on the reactions in each test tube , we

In part B, we placed 2 ml of potato extract into 5 different test tubes, which we labeled A - E, as we were working with such a large quantity of test tubes, we employed the use of a test tube rack. We then needed to set up an additional 5 more test tubes, also labeled A-E but to these we added 2 ml of H2O2. Once all ten test tubes were set up we were to divide them up and place one of each type of tube in water baths located around the lab. Each bath had an alcohol thermometer resting inside, recording the current temperature of the bath. Tube A was to be placed in the ice bath, tube B in the cold water bath, tube C in a room temperature bath, tube D in a warm water bath, and finally tube E in a boiling water bath.

The two tubes were placed into the distilled water and was heated until the temperature reaches above 45o

The objective of this lab was to examine the effect of an environmental variable on the catalytic function of an enzyme. To the point, the objective was to perform an experiment to test the effect of pH on the function of the enzyme amylase.

Methods - In the beginning of the lab, the beaker filled with boiling water was taken off of the hot place and saran wrap was wrapped around it. After that, a thermometer was placed inside of the water. Next, the temperature was checked and recorded every two minutes over a course of twenty minutes. The temperatures were recorded to see quickly the temperature of boiling water decreased over time. All of the groups did the exact same thing except with different insulators.

We began this experiment by obtaining and measuring the mass of a 20 x 150 mm test tube and a 22 x 175 mm test tube with boiling stones. We then weighed out 2 grams of zinc and iodine. First, we added the zinc to the 20 x 150 mm and weighed it out. Then we added the iodine to the 20 x 150 mm and weighed it out. This gave us the amount of each element we were working with before the reaction. Next, we made weak acidified water by adding 18 drops of acetic acid to 25 mL of distilled water in a 50 mL beaker. Then we added 5 mL of acidic solution to the 20 x 150 mm test tube. After the solution is added we swirled the test tube until the liquid became colorless. Once colorless, we poured it into the 22 x 175 mm test tube. We repeated mixing the acid with the zinc and iodine two more times. Then we repeated it 3 more times but poured the solution down the drain. Furthermore, we used a bunsen burner to softly dry off the zinc in the 20 x 150 mm test tube. Once, completely dry we let the test tube

Enzymes are proteins that functions as a catalyst in chemical reactions that occur in living organisms. The rate of enzyme activity can be effected by many factors, including pH, temperature, enzyme concentration and substrate concentration. This experiment will be focusing on the first three factors. Enzymes are sensitive and can only work under optimum conditions in order to function properly. If conditions are not optimum, the rate of enzyme activity will decrease, or the enzyme could become

Add the same amount of Benedict’s solution in each tube and for the ones that need iodine use the same amount.

So in order to see the effect of lead ions into the enzyme rate of

Half of each tube’s contents are poured into a new test tube each respectively after the tubes are incubated for 1 hour. One set of tubes is tested for: