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Detection Of Levetiracetam

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Literature Review: In the article, “A simple and cost-effective HPLC-UV method for the detection of levetiracetam in plasma/serum of patients with epilepsy,” by Engelbrecht, Grobler and Rheeders, the concentration of levetiracetam was determined using HPLC with a diode array detector. Levetiracetam is a common treatment for epilepsy. Patients have to go through regular blood tests to ensure the drug is working to optimal efficiency and is not taken in toxic levels. For the experiment, the researchers used and Agilent 1200 HPLC with a degasser, binary pump, thermostatic oven and diode-array detector with varying wavelengths. The column used was a Venusil XBP C18, 250 x 4.6 mm, and 5 µm particle size. The column was protected by a guard precolumn with a graphite filter. The flow rate of the mobile phase was 1 mL/min. The mobile …show more content…

By making a calibration curve using different dilutions of a capsaicinoid stock solution, the concentrations + standard deviation of capsaicin and dihydrocapsaicin in the hot sauce sample was determined to be 48.922 + 0.226 ppm and 6.118 + 0.272 ppm, respectively. The concentrations were then converted to Scoville units resulting in mean heat ratings for capsaicin of 133,903 Scoville units and 16,747 Scoville units for dihydrocapsaicin. The total heat rating + standard deviation was 150,648 + 917 Scoville units. Before correcting the heat rating for 7.22% analyte lost, the total heat rating was 141,717 + 897 Scoville Units. The amount of analyte lost was minimal and didn’t significant affect the results. Both heat ratings correspond to Haban ̃ero, Scotch Bonnet and Jamaican Hot peppers. The hot sauce analyzed was Tabasco Haban ̃ero sauce. The total heat rating for the experiment agreed with the heat rating of the Haban ̃ero pepper that was used to make the Tabasco

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