UNIVERSITI MALAYSIA SABAH
SCHOOL OF FOOD SCIENCE & NUTRITION
LABORATORY REPORT
NT20903 FOOD CHEMISTRY AND BIOCHEMISTRY
Determination of free fatty acid(FFA) and iodine value (IV)in oil
LECTURER NAME : MOHD NAZRI BIN ABDUL RAHMAN
LAB SESSION : 3 OCTOBER 2011 (GROUP 4, MONDAY)
Group Member
Title: Determination of free fatty acid (FFA) in oil and determination of iodine value (IV) in oil
Introduction
Acid value or free fatty acid content is an important characteristic commonly used in quality control of fat and oil. Fats and oils are one of the large variety and important sources in the world. It contains acidity which is the result of the degree of breakdown of the triacylglycerols by a chemical reaction known as
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H H H H H H H H H C C = C C H + Br2 H C C C C H H H H Br Br H
One application of the iodine number is the determination of the amount of unsaturation contained in fatty acids. This unsaturation is in the form of double bonds which react with iodine compounds. The higher the iodine number, the more unsaturated fatty acid bonds are present in a fat. In a typical procedure the acid is treated with an excess of Wij's iodine solution which a solution of iodine monochloride (ICl) in glacial acetic acid. The iodine concentration is then determined by titration with sodium thiosulfate. Iodine value is the average unsaturation measurement in fat sample. Although iodine value is expressed as the weight of iodine added to 100 g of sample, the analytical procedure used to determine the iodine normally use iodine monochloride in acetic acid solution (Wijs’ reagent) as halogenations agent.
Objective
1) To determine the free fatty acid found in oil.
2) To differentiate between the free fatty acid formed during the hydrolysis process and oxidation process.
3) To understand the relationship between acid value and free fatty acid
4) To determine the iodine value (IV) in oil
5) To
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Get AccessAlso to determine the molar mass of an unknown solid acid by titration with standardized NaOH solution. First we prepared NaOH solution and standard acid. Measure appropriate amount of 6 M NaOH. Transfer to 500 ml volumetric flask. Use deionized water to fill the 500 ml flask. Then transfer it to plastic Erlenmeyer flask. Same thing with the acid but use 250 ml volumetric flask. Weight appropriate amount of oxalic acid dehydrate, transfer and mix it with deionized water in 250 ml volumetric flask. Transfer this standard acid solution to a 250 ml plastic Erlenmeyer flask. After that standardization of NaOH by titration. Take 15-20 ml of the acid into 250 ml glass and add 2 or 3 drops of phenolphthalein. Add NaOH with constant swirling until the acid solution change into pink. Record the final and initial volume used in
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Doctors use Iodine-131. Iodine-131 can be used to treat someone with hyperthyroidism. It can also be used to treat thyroid cancer. It can also be used as a type of medical imaging.
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The purpose of this lab is testify the % fat in meat, which is Beef Franks. We use the whiley extractor unit to separate fat from protein by dissolve the fat in acetone. % fat in meat= weight of extracted fat/weigh of fresh meat *100 is used to calculate for % fat in meat in this experiment. It is a simple experiment that involved intense degree heat, cool water, meat in an extraction thimble inserted into a flask, and acetone. The percentage error is 50.6%.
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