Development and Use of the The DF-1 Cell Line

995 WordsFeb 1, 20184 Pages
The DF-1 cell line was first created in 1997, by D. Foster and L. Foster at the University of Minnesota, USA. DF-1 is a non-tumorigenic chicken embryo fibroblast cell line derived from 10 day old East Lansing Line eggs. Primary chicken embryonic fibroblasts were originally dissociated and grown in a laboratory culture setting. The cells were then passaged until they began to deteriorate, then concentrated to maintain around 30-60% confluence. The non-aging cells were then identified and grown for additional passages. This passaging resulted in the creation of the spontaneously immortalized chicken cell line DF-1. Under a microscope, live adherent cells take on a spindle shaped form, whereas dead cells will present as more rounded than spindle-like. When culturing a DF-1 cell line, the most common medium used for growth is a minimal essential medium, with 5% fetal calf serum. The cell line is then grown/incubated at 39° Celsius. The DF-1 cell line is often used in the research of avian viruses. It is unique to the group of mammalian cell lines in that it does not contain endogenous sequences linked to avian sarcoma and leukosis, a first of its kind in continuous avian cell lines. The study of avian retrovirology relies heavily on access to a continuous cell line, and the DF-1 cell line is both continuous and non-transformed. Because the cell line lacks any sequences related to avian viruses, it is most popularly used to study the effects of these viruses on cells as they
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