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Difference Between Tyrosinase And Dopa

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Methods Tyrosinase and DOPA are key elements in making melanin. In order to model this reaction, we obtained the enzyme from a 15 g peeled potato and placed the chilled enzyme into five pairs of tests tubes with different pHs to measure the amount of melanin produced. The blank test tubes consisted of 4.9 ml of pH 6.0 phosphate buffer in the blanks, 0.1 ml tyrosinase extract, and a pH of 3,5,7,9 or 11. Each reacting test tube consisted of 3.9 ml of pH 6.0 phosphate buffer, 0.1 ml tyrosinase extract, pH of 3,5,7,9 or 11 and 1.0 ml of DOPA. Each test tube was measured with a spectrophotometer at a wavelength at 450 nm at time 0 and every two minutes for fourteen minutes. A blank was put in before measuring each test tube with the DOPA. After

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