Methods & Materials There are many procedures during this lab and many materials needed for an accurate analysis of data. First, fill a 100 mL graduated cylinder with 50 mL of water. Add 25 germinating peas and determine the amount of water that is displaced. Record this volume of the 25 germinating peas, then remove the peas and put those peas on a paper towel. They will be used for the first respirometer. Next, refill the graduated cylinder with 50 mL of water and add 25 non-germinating peas to it. Add glass beads to the graduated cylinder until the volume is the same to that of germinating peas. Remove the beads and peas and put on a paper towel. They will be used in respirometer 2. Now, the graduated cylinder was filled once again, determine how many glass beads will be require to reach the same volume of the germinating peas. Remove the beads and they will be used in respirometer 3. Then repeat the procedures used above to prepare a second set of germinating peas, dry peas and beads, and beads to be used in respirometers 4,5,and 6, the only difference is the temperature of the water.
And finally into test tube 3, I pipetted 1.0 ml turnip extract and 4.0 ml of water. The contents of test tube 1 was poured into a spectrometer tube and labeled it “B” for blank. “B” tube was now inserted it into the spectrometer. An adjustment to the control knob was made to zero the absorbance reading on the spectrometer since one cannot continue the experiment if the spectrometer is not zeroed. A combination of two people and a stop watch was now needed to not only record the time of the reaction, but to mix the reagents in a precise and accurate manner. As my partner recorded the time, I quickly poured tube 3 into tube 2. I then poured tube 2 into the experiment spectrometer tube labeled “E” and inserted it into the spectrometer. A partner then recorded the absorbance reading for every 20 seconds for a total of 120 seconds. After the experiment, a brown color in the tube should be observed to indicate the reaction was carried out. Using sterile techniques, any excess liquid left was disposed
For the second part of the experiment I cut the cheesecloth into four pieces and folded them so that it was four layers thick. I placed one piece of cheesecloth into the funnel and measured 60mL of soil using the 100mL to help measure the soil and poured that into the funnel. Taking beaker number one I poured the contents into the funnel and let that filter into beaker number five. I used the same technique as above and I repeated the same thing to beakers number two through four and poured them into beakers number six through eight. Once this was done I observed beakers five through eight and wrote down my observations in Table 1.
Blood Type Blood type is the type of blood a person has that is passed down from the parents. The goal of this experiment was to find out if all the children were Mr. Johnson’s. The guided question was “Are all of Mr. Johnson’s children his biological offspring?” This relates
In a third test tube, put 1 mL of chutney. Repeat steps 2 and 3 with the chutney and record the results in Table 3.
The control of the experiment is water. The positive results are the juices that turned into jelly and the negative results were pineapple and kiwi.
LAB REPORT 4 Observations of Chemical and Physical Change PART 1 – OBSERVATIONS OF CHEMICAL CHANGE No credit will be given for this lab report if the Data section is not completely filled out and if the required photographs are not received. At least one photograph must show the student’s face. OBJECTIVES 1. Observe
The purpose of this lab was to determine if lima beans would be able to grow nice and big in gatorade. The hypothesis stated, if we put the lima bean in the gatorade then it will grow really big, fast because gatorade contains of electrolytes that might affect the lima beans growth. The hypothesis turned out to be disapproved because the lima beans average length in the water/ control starting from day 1 was 17.5 mm, 25.4 mm, 42mm, and 44mm. The lima beans average length in the gatorade was 17mm, 22.6mm, and by day 3, the lima beans grew mold. In addition to that, The total mass of the lima bean in the control was greater than the bean in the variable. The lima bean in the control’s total mass was 5.25g, 9g, 11.9g, and 20.5g. The lima bean
Madeline Jaquez Methods For this experiment, we used two restriction enzymes called Ava II and Pvu II. Four tubes were needed for the experiment as well. Each microtubule was labeled accordingly, A, B, and C. Using Micropipettes, we added 2 microliters of pUC19 DNA into each tube. To make sure the DNA was on the bottom of the tube, we tapped each tube on the lab bench. Each tube had its own specific amount of different solutions added on, however the final volume for each tube was 30 microliters. Tube A had, 24 microliters of DI water, 3 microliters of the buffer, and 1 microliter of the Ava II enzyme. Tube B had, 24 microliters of DI water, 3 microliters of the buffer, and 1 microliter of the Pvu II enzyme. Tube C had, 23 microliters of DI Water, 3 microliters of the buffer, 1 microliter of Ava II, and 1 microliter of Pvu II. The last tube C had, 25 microliters of DI water, 3 microliter of the buffer, and no enzymes. Each solution was added according to how it is written here. After we finished adding the solutions to each tube, we tapped the tubes on the lab bench to make sure the
The experimental procedures for Lab 2 were provided on Blackboard labelled as “Pre-Lab 2: Techniques & Measurement”.
An unknown was given to our group from the professor. The unknown was in nutrient broth, the group received unknown number 3. And the task was to identify the unknown and try to make an educated guess, and identify the unknown #3. A wide variety of processes had been
Methods and materials The first experiment begun by filling a 600-ml beaker, almost to the top, with water. Next, a 10-ml graduated cylinder was filled to the top with water. Once water was added to the beaker and graduated cylinder, a thumb was placed over the top of the graduated cylinder. This would ensure that no water was let out and no bubbles were let into the graduated cylinder. Next, it was turned upside down and fully submerged into the beaker. Then, a U-shaped glass tube was attained. The short end of the glass tube was placed into the beaker with the tip inside of the graduated cylinder. Next, a 50-ml Erlenmeyer flask was received. After, 10-ml of substrate concentration and 10-ml of catalase/buffer solution were placed into the flask. A rubber stopper was then placed on the opening of the flask. After adding these, the flask was held at the neck and spun softly
Electrophoresis Gels A total of three gel runs were made to see if there were any fragments that indicated the presence of bacteroides. The Bacteroides products were to fall within 530 base pairs to be considered a positive result. The first gel pictured, Figure 1, on the left was the first run made to test the 1/10 dilution of Turtle Creek with sewage water, but only the ladder appeared. The second gel represented, on the right on Figure 1, a repeated test of the first gel; witch indicated the successful extraction of DNA. The last two pictures on Figure 2 are the same gel just emphasizing a bit more on the bands that were faint. This gel had a total of five DNA samples plus the controls. As indicated in the image below there was three out
Then, each group of students received the necessary materials to complete the experiment. When the students received the cups, they labeled cups to distinguish between the salt solution, distilled water, and control group. After weighing the cups and finding the mass of the cucumbers, the students poured 50 ml of water in one cup, 50 ml of salt solution in the other, and left the control cup empty. Then, the students placed the cucumbers into the cups and waited 30 minutes for the results. After the 30 minutes, the students removed the cucumbers from each solution and dried the cucumbers with paper towels. The students then weighed the cucumbers again and recorded their results. Lastly, the students found the difference from the original mass of the cucumbers and recorded their results.
The experiment that the class worked on was about peroxidase. Peroxidase is part of the enzyme group that presents most living organisms (Ahmed, 2013). Peroxidase interferes with the removal of hydrogen peroxide (Ahmed, 2013). Hydrogen peroxide is a toxic product that have normal metabolism before it causes any cell damages (Ahmed, 2013). Peroxidase has two substrate and both of them must present a reaction (Ahmed, 2013). One of the two substrate is H2O2 and other one just depends on the organism or the cell type (Ahmed, 2013). The substrate that the class uses is turnip extract. In the class there were five experiments to do but the class were assigned into groups and each group were going to do two experiment. The names of the experiments are: Baseline, Temperature, and pH.