Different Methods, Bacteria, And Development Transduction, Transduction And Transformation Transduction

One of the most imperative functions in maintaining the development of evolution is the frequency of genetic transformation: the injection of foreign DNA into another organism’s DNA. This term is defined by the actions of a vector, but more specifically by the actions of plasmids and phages. However, in this experiment we are primarily focused on the effect of the pGLO plasmid transformation of GFP on the E. coli bacteria by introducing a second chromosome or a plethora of cloned plasmids. (Bassiri 2011)

Name one endogenous source of contamination and discuss the modes of transmission from the source to the new host.

For millions of years’ bacteria have been surviving to their environments and been at war against other microorganism. This wars have lead them to creating an unstoppable arsenal of weapons they can use to survive. Unlike virus that need a host cell to survive, bacteria can survive anywhere since they can share their DNA with each other. This allows bacteria to survive in places like radioactive waste, zero oxygen environments, and even in absolute darkness. Unlike human beings who are born with a specific genetic code, bacteria have the capability of changing that code. Bacteria have three methods of evolving themselves in order to survive the harsh is of conditions. The three methods are called horizontal gene transfer. The first is Transformation, but some pathologists call it the “Funeral Grab”.

In two weeks of genetic transformation, we were able to successfully complete the objectives of the lab by correctly performing a transformation of bacterial cells. During the lab, we were able to successfully complete the components that were necessary for a transformation of bacterial cells. Viable cells were transformed, able to grow, and were put in a sterile environment conducive to growth. With the exception of the plate labeled LB/Amp: -pGLO, our results followed the lines of a successful experiment. The LB/Amp: -pGLO plate was contaminated and had colony growth in it. In order for the transformation to be completely successful the LB/Amp: -pGLO plate would have had zero colony growth. As stated in the laboratory manual, biotechnologists

Transformation of Escherichia coli to Form a Recombinant Plasmid Containing Genes for both Ampicillin and Kanamycin Resistance

The transformed bacteria showed growth despite the presence of ampicillin (Table 1), whereas the control plate with ampicillin did not show any growth, and the control plate with only LB agar showed the formation of a lawn of bacteria (Table 2). The transformed bacteria on the plate with LB, ampicillin and arabinose differed from the transformed plate without arabinose in that they glowed green under UV light. The bacteria without arabinose maintained an unaltered appearance under UV light. The transformation efficiency for the transformed bacteria was 5.2 × 104 transformants per microgram of DNA.

Through performing bacterial transformation using the pB325 plasmid, the expected result was to see growth on all plates with +DNA (four plates) and two -DNA plates, the plates with no amp added. This was expected since +DNA E. coli were protected from amp and could grow on normal plates. The -DNA plates with amp were expected to have no growth as the E. coli were not protected against amp. The result of this experiment yielded a lawn of bacteria on all LB plates while none of the LB+amp plates yielded any growth. These results suggest that the experiment did not work as intended. This is because transforming the E. coli with pB325 plasmid was expected to offer those cells protection against amp. Thus, it can be hypothesized

The purpose of this lab was to purify and test a GFP protein via several laboratory methods for the purpose of purifying and testing the protein in SDS-PAGE. To purify the protein chromatography and gel electrophoresis were the methods used in the experiment. GFP in the samples were tested using an ultraviolet light. When GFP was found present the cell were transformed into a petri dish containing ampicillin and arabinose. The cells were then lysed and SDS-PAGE was used to test.

the desired gene is inserted into the plasmid and the plasmid is returned to the bacterium by transformation.

In the following experiment, Escherichia coli were transformed using six separate agar plates. Transformation occurs when plasmid DNA is uptake into the bacterial cell. The two ways transformation is facilitated is by placing them in calcium chloride (CaCl2) and heat shock. These two methods allowed the bacterial cells to become competent or more open to the uptake of plasmid DNA. The purpose of this experiment was to transform bacterial cells with plasmid containing ampicillin resistance and lux genes. It was hypothesized that the lux genes would have created a light-emitting reaction on the bacteria while the ampicillin genes should have made the bacteria resistant to ampicillin attacks and be able to grow normally. The experiment results

Introduction: Transformation is the process where a foreign plasmid is inserted and to bacteria. The bacteria eventually intensifies the plasmid and produces a large amount of it. A plasmid is a circular DNA strand that is utilized for growth and bacteria.

. The way this process works of transferring and transducing bacteria and it’s DNA is used in different cycles. The lysogenic cycle, the lytic cycle, Binary fission, transduction, transformation and conjugation. All of these are what transfer bacterial DNA from one host cell to another

In order to introduce foreign genes into bacteria a 3-step process must occur. First scientists must use restrictions enzymes (or polymerase chain reaction if there is not enough restriction enzyme sites) to remove the foreign gene from the DNA sequence. Then they must use the same restriction enzymes to cut into the circular DNA sequence in the plasmid. Finally the foreign gene is placed into the opened

Bacterial transformation is the process of moving genes from a living thing to another with the help of a plasmid.The plasmid is able to help replicate the chromosomes by themselves; laboratories use these to aid in gene multiplication. Bacterial transformation is relevant in everyday lives due to the fact that almost all plasmids carry a bacterial origin of replication and an antibiotic resistance gene(“Addgene: Protocol - How to Do a Bacterial

Genetic transfer. The bacteria in biofilms readily take part in gene transfer or exchange of extra-chromosomal DNA (Donlan, 2002).