Introduction
A dialysis membrane is a semi-permeable membrane that is used as a barrier that facilitates the exchange or to impede the travel of material or molecules through the process of diffusion and/or osmosis. Our own body cells have a plasma membrane that controls the movements of substances into and out of the cell (Tortora, 2017). Diffusion is the movement of any solute or particle from one place to another. However, osmosis entirely refers to the movement of water across the membrane. Osmotic pressure refers to the minimum pressure that needs to be applied to a solution to prevent the inward flow of its pure solvents water across a semi-permeable membrane (Tortora, 2017). Our body controls water amount through both osmotic and hydrostatic
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After the first 15 minutes the data is showing that there is movement between the dialysis bag and the hypotonic water. At this point it was indeterminate as to if the sucrose solution moved into the water by diffusion or not. We noted that the dialysis bag had gained weight at each 15-minute interval. At each interval the weight gain was slightly less from the previous weight gain. The most weight gained was in the first measurement when the gradient was the highest with the weight of the dialysis bag increasing 2.1grams with a 10.16% change. We determined that there was no change in the water clarity in the beaker. We determined that the movement of fluids in and out of the dialysis bag was by osmosis and not by diffusion of the sucrose. Our hypothesis as to the net movement of water by osmosis was correct, conversely, our hypothesis as to the chemical movement by diffusion was incorrect. The weight of the dialysis bag increased at each interval, yet there was no change in the color of the water in the beaker suggesting that there was no diffusion. One possible explanation as to the lack of diffusion would be that the sucrose and red dye concentration were not concentrated enough to be observable. Alternatively, the dialysis bag did not have time to reach a point of equilibrium and become isotonic with the solution in the beaker. Potentially a repeat of this experiment using higher concentrations of red dye and sucrose would achieve different results as to an observable diffusion of chemicals inside the dialysis bag into the beaker of water, it might also have an increase in the osmosis
Secondly, osmosis was to be observed to gain a proper understanding of how the principal of dialysis functions.
In this experiment, we will investigate the effect of solute concentration on osmosis. A semi‐permeable membrane (dialysis tubing) and sucrose will create an osmotic environment similar to that of a cell. Using different concentrations of sucrose (which is unable to cross the membrane) will allow us to examine the net movement of water across the membrane.
The Diffusion and Osmosis Lab determines the molarities of various sucrose solutions based on change in mass. Using table sugar in different amount of molar concentration 0.0M, 0.2M, 0.4M, 0.6M, 0.8M and 1.0M. The molarity of the solution of the sucrose solution in the dialysis tubing determines the amount of water that either move into the bag or out the bag, which also means its mass changed.
In which two of the dialysis bag contain tap water. The three other Bags were 20% sucrose 40% sucrose and 60% sucrose. To perform this lab we prepare each of 5 bags with their designated sucrose solution. In this experiment the dialysis tubing acted as selectively permeable membrane. The dialysis bag allows the passage of water molecule only because the sucrose molecule are too large to pass through the membranes. After filling those bags ,we then placed the bags into five separate beakers. Four of this beakers contained only tap waters, and the fifth beakers contains solution of 60% sucrose. We filled the first dialysis bag with 10 ml of tap water, next we filled the second bag with 10ml of 20% sucrose, the third bag was filled with 10 ml of 40% sucrose solution while the fourth bag was filled with 10ml of Tap water (H2O). We made sure that when filling this bags that we removed as much air as possible before clamping of the end of each bag. We also made sure that all of the bags rest soft and floppy instead of firm to ensure that experiment will work properly. Next we placed dialysis bag ,one through four in their own separate beakers which was filled with tap water (H2O), and our fifth bag was full of tap water was placed in beaker filled with just enough of the 60% sucrose solution to cover the bag. All of these bags remained in their designated solutions for 45 minutes. However, all of this bags were quickly removed every
As the lab introduction explains, osmosis is relatively permeable to water and will follow solutes. By instinct, the water will move from a more diluted solution to more of a concentrated solution. The products of the experiments concluded the physiological significance of osmosis by how cell membranes in the body are semipermeable meaning that only certain molecules can pass through it. When intracellular fluid and extracellular fluid are at equilibrium by non-penetrating and concentrated solutes, no net movement of water goes in and out of the cell. Furthermore, if the ECF changes in osmolality, then depending on the difference between the ECF and ICF will determine whether water moves in or out of the cell. This is important in the cell membrane as small differences in osmolarity correspond to large, rapid change in osmotic pressure, causing cells to gain or lose water. In sum, our body makes critical decisions in what molecules are allowed to penetrate the cell membrane and make sure that our red blood cells don’t cause any problems within the
The purpose of this lab was to observe the osmosis rates and mass changes of dialysis tubes. To this three-dialysis tubes with differing sucrose levels were tested on their rate of osmosis and weighed at 15-minute intervals. The results found described that as the sucrose level increases the rate of osmosis increased as well.
The independent variable was the concentration of sucrose in the dialysis tubing we used as a simulated membrane.
Table 1 shows the contents of the bags and the content of the concentration it was submersed in. Bags 2-4 each contain a solution of both sucrose and water. These bags were each put into beakers containing hypertonic solution. These bags gained weight over time because the water moved from its high concentration inside the beaker to the low concentration inside the membrane of the artificial cell, the membrane being the bags that consisted of dialysis tubing. The
The major objective of the experiment was to test the effect of the concentration gradient on the diffusion rate. It was hypothesized that the greater the stronger the concentration gradient, the faster the rate of diffusion would be. To test this, dialysis tubes were submerged in different concentration fructose solutions. We weighed the tubes at specific time intervals to measure the rate of diffusion of water in each different solution. The results illustrated that increased concentration gradient increases the rate of diffusion of water in the tubes. We concluded that as concentration of the
In this study we constructed we researched whether different sucrose concentrations affect the rate of osmosis. In order to do this, we constructed artificial cells out of dialysis tubing filled with 20% sucrose and 40% sucrose and weighed them every 10 minutes for 90 minutes. In doing so, we concluded that the higher the sucrose concentration, the faster the rate of osmosis.
The different solutions effected the osmotic rate depending on wether or not it contained the catalyzing enzyme, invertase, which helps break down sucrose. The blue solution showed an increase but overtime the rate slowed down. I believe this is because as glucose and fructose were leaving the cell water was also crossing over the membrane. It takes time for invertase to break down sucrose resulting in the slight increase in osmotic pressure. However, I
Six plastic cups were obtained and labeled 0.2 M, 0.4 M, 0.6 M, 0.8 M, and 1.0 M. Next, each of the six dialysis tubes were knotted on one end and filled with the sucrose samples, and then tied off. These samples were then dried by patting with a paper towel, weighed and placed into their corresponding cups. The mass of each sample was recorded in table 2 on the data sheet. Each cup was then
Hypothesis: If we add higher concentrations of sugar to the dialysis tubing, then the net movement of coffee into the dialysis tubing will increase.
The hypothesis states that if the solution is hypotonic the results will decrease, if the solution is hypertonic the results will increase and if the solution is isotonic the solution will vary and or remain constant. In order to test the predictions of the hypotonic, hypertonic, and isotonic hypothesis for the solution made during the study, four samples of sucrose were taken and placed into two different beakers each containing a different concentration. Then dialysis tubing A was placed into beaker 1 with B, C, and D placed into beaker 2 for 45 minutes and weighted at 15 minute intervals. My finding in the study was that each of the four samples changed from their initial weight and for the most part accurately proved the hypothesis.
Both the Freud’s and Erik’s first stage have similarity since they both refers this stage