Diffusion and Right Beaker Essay

1005 Words Oct 16th, 2012 5 Pages
PhysioEx™ Lab: Cell Transport Mechanisms and Permeability

Activity 1: Simple Diffusion
Chart 1 Dialysis Results (Average Diffusion rate in mM/min)
Solute Membrane (MWCO) 20 50 100 200
NaCl (—) 0.0150 0.0150 0.0150
Urea (—) (—) 0.0094 0.0094
Albumin (—) (—) (—) (—)
Glucose (—) (—) (—) 0.0040

1) Which solute(s) were able to diffuse into the right beaker from the left? NaCl, Urea, Glucose
2) Which solute(s) did not diffuse?
Albumin

3) If the solution in the left beaker contained both urea and albumin, which membrane(s) could you choose to selectively remove the urea from the solution in the left beaker? How would you carry out this experiment? 16 min 200 = 4.5 in both beakers 16 min
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The filtration rate increased as the solute number increased

2) Which solute did not appear in the filtrate using any of the membranes? Powdered Charcoal

3) What would happen if you increased the driving pressure? Use the simulation to arrive at an answer. The concentration remained the same but the filtrate rate increased.

4) Explain how you can increase the filtration rate through living membranes.
By increasing pressure which increases filtration rate.

5) By examining the filtration results, we can predict that the molecular weight of glucose must be greater than 100 but less than 200.

Activity 5: Active Transport

1) Watch the solute concentration windows at the side of each beaker for any changes in Na+ and K- concentrations. The Na+ transport rate stops before transport has completed. Why do you think that this happens? The Na/K concentrate have equalized.
2) What would happen if you did not dispense any ATP?
No transport will

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