Digestion Lab

1610 Words Apr 4th, 2011 7 Pages
Digestion Lab
Abstract – The purpose of this lab was to understand how different solutions played a role in the digestion protein. By looking at different variables, such as temperature, and pH we’re capable of understanding just how certain substances functioned and when they didn’t. The data for all labs are clear and concise and give a clear understanding of what solutions work best. All three labs were placed in a warm water bath set at 37’C to stimulate the reaction as if it were taking place within the human body. This gives us a more accurate reading on how they would react at that set temperature. We concluded why certain tubes changed to the color they did and further explained it. This lab focuses primarily on two crucial
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Enzyme pepsin
X. Gastric juices
XI. Incubator (warm water bath)
Outline
[Gastric Digestion]
1. Label the 3 test tubes with your initials and number each 1,2, and 3. (This allows for them to be distinguished)
2. Measure out one scoop of albumin powder (rich protein) and place it into each individual tube.
3. Add 5mL of 0.4% pepsin solution to test tube 1
4. Add 5mL of 0.2% HCl to test tube 2
5. Add 5mL of gastric juices (contains both pepsin solution and HCl) to test tube 3
6. Stir all tubes slowly and carefully; mix contents well
7. Gently place the tubes in a 37’C water for an hour.
8. After the hour has passed, remove the tubes and add 2-3 drops of biuret reagent to each tube and shake.
9. Record the color change that occurs.
[Part A]
1. Retrieve three more test tubes and label them 1,2, and 3
2. Add 5 mL of 5% starch solution to each tube
3. Add 5 mL of pancreatic solution to test tube 1
4. Add 5 mL of water to test tube 2
5. Add 5 mL of boiled pancreatic solution to test tube 3
6. Gently shake the tubes to mix the contents inside
7. Place the tubes in 37 degree water for 30 minutes
8. Remove the tubes and add 2-3 drops of Iodine – potassium – iodide solution to each tube.
9. Again, gently shake the tubes to mix the contents
[Part B]
1. Retrieve two more test tubes and label them 1, and 2
2. Add 10 mL of litmus milk (PH Indicator) to each tube
3. Add one scoop of pancreatin powder to test tube 1
4. Add a few drops of distilled water to test tube 2
5. Gently

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