Direct Analytical Sample Quality Assessment (DASQ) for Biomarker Investigation

591 WordsFeb 16, 20182 Pages
There are several important factors to increase the chances that a proteomic study based on CSF biomarkers becomes successful. For proteomics analysis, the standardisation of CSF collection protocols is a mandatory step to search and identify new biomarkers indicators of a disease status. This point represents a milestone especially when samples are collected and stored in different centres. There is the need to select and include, in biomarker research studies, only well characterized and high quality samples with a documented sample collection and handling. Our study undelines the importance of a standardized protocol, based on some specific procedure of sample collection and storage, in order to have a successful biomarkers analysis. Moreover, it has been highlighted how MALTI TOF MS analysis of specific proteins could suggest the selection of the best pool of samples for a powerful biomarker study. We retain that DASQ needs to be used to screen the samples to be enrolled in order to reduce the number of false positives in biomarker discovery. Due to the characteristics of MALDI, faster and less expensive than other techniques, this protocol could represent a first step in the qualitative screening of the CSF before biomarkers studies. New advanced techniques appear to be particularly promising for biomarker qualification and verification. Information about these CSF proteins can be used to develop multiple reaction monitoring (MRM) based analytical assays, which offers

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