Disadvantages Of Somaclonal Variation

Asexual Propagation is the process of using plant materials such as the stems, leaves, and roots to multiply the number of plants. These plants eventually grow to be a brand new plant that is genetically identical to the parent plant it came from. In several types of plants, asexual propagation is the fastest means of new plant growth. Asexual propagation is also a good way to maintain a plant species because they are genetically identical. In this process, adventitious roots are seen in the growing cycle. Adventitious roots are those that grow form parts of the plant that they normally would not grow from. The cuttings must do

The fatal flaw in my experiment of cloning a single plant, creating a population of 100 plants that are genetically identical, and raising these plants in identical environments with no variation in traits is that there is no variation to show any difference(s) in the experiment.

Three Waves of Variation Study: The emergence of meaning in the study of variation Penelope Eckert Stanford University

This report presents an overview to: meiosis, chromosomes, traits, genotypes, and phenotypes displayed in the evolution of fast plants. Studying how the genetic information can be passed along one generation to the following. Also, learning various techniques to determine the possible genotypes of the four Wisconsin Fast Plants provided by analyzing the offspring and observing the phenotypic variation within them. Predicting that the parent plant was heterozygous with the first generation also displaying heterogeneous characteristics of non- purple stem/ Green leaves. And discovering that the null hypothesis was rejected for the chi-square being less than 5% meaning the observed phenotypes were due by chance.

Round seeds (R) are dominant to wrinkled seeds (r), and yellow seeds (Y) are dominant

might affect the fitness of each variant. In other words which factors might increase plant growth, survival,

The experiments purpose was to understand and observe the gene expressions in the genes pCNT103, cig1 and GapC in the shoot, root and callus tissues of the tobacco plant, Nicotiana tabacum. Using various genetic laboratory research techniques completed the experiment. The experiment consisted of four parts. The sterile tissue culture technique was used to differentiate the tobacco callus tissue in

Duke, S. O. (1995). Herbicide-Resistant Crops: Agricultural, Economic, Environmental, Regulatory, and Technological Aspects. 83-84. Boca Raton, Florida: CRC Press.

This procedure has also been performed successfully in the lab with dicots, broadleaf plants, soybeans and tomatoes for many years. Through this procedure, the desired gene and marker is inserted into the tDNA of the plasmid. Tissues of the organism are then transferred to a medium containing an antibiotic or herbicide in order to tell if the organism has successfully taken up the desired gene because only the tissues expressing the marker will survive. These tissues are then grown under controlled environmental conditions in tissue cultures containing nutrients and hormones so that whole plants are grown. When plants are grown and have produced seed, an evaluation of the progeny is done making sure that the desired traits have been passed on (Understanding GMOs).

Critics fear that continual use of chemicals could result in “super weeds” and “super bugs”.

Making the onion tip root cell slide was successful. Our results supported the hypothesis because we saw cells in the onion root tip in prophase, metaphase, and anaphase. As we went up in power objectives, each phase of the cell became more definitive. The cell root was a great indicator of the structures of the different cycles of the cell. This is important because we will be prepared for future labs working with the microscopes and can now adjust it for the best view of the slide. We practiced working with the compound light microscopes and different phases of the cell cycle. Onion root tips are useful to observe mitosis because the cells are frequently diving as the root grows. So when we stained the cell, we caught many cells in different phases. The significance of this lab was to better understand the process and stages of mitosis and meiosis and compare and contrast the mitotic process in plants and animals. We grasped the concepts of what the chromosomes look like, and what they look like in each step of the processes. Having read much about mitosis and meiosis, seeing these cells was the real application of describing and understanding the stages.

The source is relevant to the topic of genetic modification and the information can be used as a source of information. This article can be used to discuss the positive and negative effects of genetic engineering in agriculture. The information is not

This data shows a strange outcome, in the hypothesis; it says that “If acid is introduced to the seed during germination, then the roots will not grow as long as the seeds that are given water”. This statement proves to be untrue, because the roots grew longer with stronger acid than weaker acid, and in some, cases, grew better with strong acid than it did in water. This may be true because of the acid growth theory. The acid growth theory states that auxins cause the elongation of stem cells by promoting wall loosening. It was determined that this wall loosening is caused by hydrogen ions. This idea and subsequent supporting data gave rise to the acid growth theory, which states that when exposed to auxins, susceptible cells excrete protons into the wall at an enhanced rate, which in turn decreases the pH in the wall. The lowered wall pH then activates the wall loosening process which is essentially doing the same thing as the auxin hormone.

Instead of transferring large blocks of genes from donor plant to recipient, small isolated blocks of genes are put into the plant chromosome through biolistics, vectors, or protoplast transformation (Horsch 1993). Biolistics is a technique that shoots the gene block into the potential host cell. In order for the process to succeed, the microscopic particles and DNA must enter the cell nuclei and combine with the plant chromosome. Biolistics is commonly used but has a slight failure risk since the breeder has little control over the destination of the gene block (Mooney & Bernardi 1990). Bacteria or viruses can also carry the gene blocks into a new cell. Common vectors in gene transfer between plants are Agrobacterium tumefaciens and Agrobacterium rhizogenes. In the soil, the bacteria will infect the plants with their own plasmid, transferring the desired gene that was placed in the bacteria's DNA. Vector gene transfer is a preferred method of transformation since this modification already occurs naturally in the environment (Rudolph & McIntire 1996). Last is protoplast transformation, which uses enzymes to dissolve the cellulose in the plant wall that leaves a protoplast. Once a specific gene block is added to the protoplast, the cell wall will re-grow into a transgenic plant.

The greater the variation in DNA sequence, the easier it is to generate polymorphic markers. Therefore the plant species used for SCAR development should have sufficient variation for te traits of interest at both the DNA sequence and phenotypic levels. Researchers over the years have employed various non-sequence primed PCR markers to develop SCAR markers for the identification of sex in dioecious species of crop plants.