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The name of enzyme involved in this experiment is salivary amylase, which presents in our saliva. Salivary amylase catalyses the breakdown of complex sugar, such as starch, into a simple sugar, such as glucose. In this experiment, the optimum temperature for salivary amylase to function is 37˚C. Above the optimum temperature, the kinetic energy in the substrate and enzyme increases. The number of collisions between substrate and enzyme also increases, but the formation of enzyme-substrate complex decreases. This is because the increasing of kinetic energy causes the amino acid molecules to vibrate violently. Some bonds like hydrogen and ionic bonds which hold the configuration of the enzyme break. The shape of active site of the enzyme…show more content…
In solution B, reducing sugar is absent because the result for Benedict’s test is negative. However, the result for iodine test is positive, which indicates starch is present in solution B. In test tubes 1 and 3, reducing sugar is present after reacted in 37˚C and 95˚C respectively because it show positive result when tested with Benedict’s reagent. So, the structure of the sugar in solution A is simpler, while the structure of the sugar in solution B is more complex as it needs salivary amylase or hydrochloric acid to hydrolyze before it gives positive result for Benedict’s test. Iodine test is used to detect the presence of starch in a solution. When starch presents, iodine changes its colour from yellow to dark purple. Benedict’s test is used to detect the presence of reducing sugar in a solution. When reducing sugar presents, a brick-red precipitate appears in the solution. Benedict reagent contains copper (II) ions, which is blue in colour can be reduced to copper (I) ions, which is insoluble brick-red precipitate when reducing sugar is present. Carbohydrate B is more complex than carbohydrate A, because salivary amylase or hydrochloric acid is needed at 37˚C and 95˚C respectively to be hydrolyzed into monomers before it gives positive result for Benedict’s test. In conclusion, solution A contains simple sugar and solution B contains starch.

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