Dna And The Human Insulin Gene

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After preparing the target DNA, scientists then insert the insulin gene into the plasmid ring. Prior to insertion, the plasmid must be cut open. Special enzymes are required to cut DNA; these enzymes are generally referred to as restriction enzymes (Rediscovering Biology, 2013). After the plasmid is prepared, scientists insert the insulin gene into the ring. The ring is then closed. The human insulin gene is now recombined with the bacterial DNA plasmid. Now that the bacterial DNA contains the human insulin gene, it’s inserted into a certain variety of bacteria. It’s important for scientists to verify the success of their gene transfer to ensure that it’s capable of being reproduced. To make certain, scientists will insert the insulin gene along with a gene coding for antibiotic resistance onto a plasmid. The engineered bacteria will then be grown in a lab using a special gel that contains the antibiotic and other nutrients required for growth. Only bacterial cells containing the antibiotic resistance gene that survive are considered successful (Recombinant DNA, 2004). Multiple plasmids are inserted into each bacterial cell, improving the chances of success in terms of insulin production. As the bacteria cell thrives and begins to reproduce, its inner processes activate the gene for human insulin prompting insulin production (Kamionka, 2011). As the cells reproduce, so too does the human insulin gene. Finally, these bacteria-produced human insulin protein molecules are
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