Dna Extraction

1806 Words Jun 27th, 2011 8 Pages
Introduction
DNA (Deoxyribose Nucleic Acid) is a nucleic acid that has many names, each representing the phases that it undergoes (chromosomes, chromatin, genes/alleles); it resides in the nucleus (bound by 2 *phospholipid bilayers) of almost every cell in the body (red blood cells being an exception). DNA (your genotype) is double stranded and is responsible for replicating (from 46 to 92) during Interphase, so that mitosis can make new cells, repairing and allowing for growth in the body. It is also responsible for transcription and translation, a series of processes that allows for the genotype to become a phenotype (what you look like and metabolic processes). DNA is ~ 2 M long, and yet fits into a cell that is ~ 100 µM in size! Simple
…show more content…
Also wash out the cap, then place them on the drying rack. Rinse out the funnel and place it on the drying rack as well. Wipe down the desk with cleaner and a paper towel. Throw the paper cup and pipette away in the regular trash bin.

Evaluation

1. Describe what you see inside the test tube after you allow the solution to sit with the sports drink and cheek cells for 2-3 minutes.

The DNA solution mixed with the sport drinks but not much precipitation, and not much of a change at all, a bit cloudy, but nothing too radical.

2. Describe what you see inside the test tube when you add the cold alcohol to your cheek cell solution.

Ethanol, the cold alcohol we used is non-polar. When DNA (our cheek cell solution with sports drink) is placed into a solution of 100% Ethanol, it became insoluble and precipitated out of solution. The coldness also stop the DNA strands from breaking apart. According to our textbook, DNA strands are held together by "fragile" hydrogen bonds. Adding ethanol decreases the dielectric constant of the solution.
The tube with the DNA/Cold Alcohol mixed had numerous tiny air bubbles freed from the alcohol which was warming up. And quickly, there were less bubbles and the DNA is observed as a milky substance.

3. After allowing the solution to sit undisturbed for 15 minutes, describe what you see. How does this differ compared with your initial observation?

Since we kept

More about Dna Extraction

Open Document