Dna Extraction

Purpose: The purpose of this experiment is to observe chemical changes in common consumer products to determine if the chemicals are basic, acidic, or remain neutral when mixed with other chemicals.

The first step that needed to be done in this experiment was adding hydrochloric acid (HCl)

To perform this test, a small drop of water is placed on a clean microscope slide. A metal loop that has been properly sterilized in the blue flame and allowed time to cool is used to

After adding all four reactants and keeping the mixture cold a cloudy, white residue was present in the bottom of the flask.

Heavy precipitate emerged immediately and solution turned white in color; solution then became opaque and turned light, bright blue in color.

Place the test tube containing cold water in a test tube clamp and hold the test tube above the burning alcohol. Observe the outside of the test tube for evidence of product formation.

1. (10 points) Write out your pre-lab prediction, including what evidence or previous knowledge you have to support that prediction.

Each human being has something called DNA. DNA is described as genetics and an extremely long macromolecule that is the main component of chromosomes and is the material that transfers genetic characteristics in all life forms. DNA constructs of two nucleotide strands coiled around each other in a ladder like arrangement with the sidepieces composed of alternating phosphate and deoxyribose units and the rungs composed of the purine and pyrimidine bases adenine, guanine, cytosine, and thymine. Each chromosome consist of one continuous thread-like molecule of DNA coiled tightly around proteins and contains a portion of the 6,400,000,000 basepairs that make up your DNA.

What is DNA? Is it these winding strands that look like ladders or is it what gives a person blonde hair and blue eyes? Actually, DNA is both of these things. DNA is a person’s genetic makeup–their hereditary blueprint passed on by their parents. It is a part of almost every cell in the human body. In each cell, a person’s DNA is the same; it stays the same throughout their lifetime. DNA is found in skin tissue, sweat, bone, the root and shaft of hair, earwax, mucus, urine, semen, and vaginal or rectal cells. The DNA found in a person’s saliva is the same as the DNA found in their blood. Parts of the DNA determine our physical characteristics, such as eye and hair color, height, and bone structure, but the

The materials used for the first part of the experiment comprised of the following: a microscope, 4 slides, 4 slide covers, blood samples, lancet, a sheet of paper towel, 3 test tube droppers, Solutions A, Solutions B, and Solution C.

A drop of blood is added into each test tube and they are left for 5 min.;

Let the test tube sit undisturbed for 2 - 5 minutes. You should begin to see air bubbles form at the boundary line between the ethanol and the filtered fruit solution. Bubbles will form near the top, and you will eventually see the DNA float to the top of the ethanol. Gently insert the stir stick into the test tube. Slowly raise and lower the tip several times to spool and collect the DNA. If there is an insufficient amount of DNA available, it may not float to the top of the solution in a form that can be easily spooled or removed from the tube. However, the DNA will still be visible as white/clear clusters by gently stirring the solution and pushing the clusters around the top. Post-Lab Questions What is the texture and consistency of the DNA DNA is viscous and greasy. Why did we use a salt in the extraction solution High salt makes DNA less soluble in water. In order to dissolve, the water needs to interact with the DNA. Since DNA is quite large, it needs to interact with lots of water for this purpose. When you add salt, the water preferentially interacts with the salt (its small, and can move around in solution easier than the DNA can). This makes it so there is less water available to interact with the DNA and it becomes less soluble. Is the DNA soluble in the aqueous solution or alcohol DNA is less soluble in an alcohol such as isopropanol than it is in water. This is because alcohols are non-polar, whereas water is polar. The polar

Name ____________________________ I) Introduction All cells contain four major types of macromolecules: carbohydrates, lipids, nucleic acids, and proteins. In today’s lab, we will be studying three of the four-proteins, carbohydrates and lipids. Various chemical tests can be used to detect the presence of each of these molecules. Most of the tests involve a color change visible to the eye. If a color change is observed, the test is considered positive. If the color change is not observed, the test is negative, indicating that a particular molecule is not present. In all the chemical tests we will be performing, we will also be using a control. In most cases, the control will be a sample of

An alcohol bath was prepared with an ethanol solution and dry ice, the solution was measured using a thermometer, at approximately -15˚ the bath was placed under a Polaroid filter and above a stable jar upon a white piece of paper. The capillary level holder with the frozen capillary tubes was placed carefully into the bath (at an angle to reduce effervescence). A lamp was placed 20 cm away from the setup and shone on the Polaroid filter. This setup ensured the ethanol solution didn’t heat up too rapidly.

Due to the DNA’s specificity, samples can be utilised for identification. DNA is a nucleic acid composed of deoxyribose sugar bound to a phosphate group and one of four nitrogenous bases (adenine, guanine, cytosine and thymine). Each section of these three components are referred to as nucleotides, which are joined to the phosphate or sugar of another nucleotide by strong covalent bonds to form a backbone. The nitrogenous bases are joined to complimentary bases of another nucleotide (adenine with thymine, guanine with cytosine) to create a double stranded molecule (Figure 2). To complete the double helical structure, the molecule coils to compact it’s contents. DNA molecules can contain up to two million base pairs, with a human genome containing approximately 3 million base pairs. The random assortment of nitrogenous bases as well as the numerous mutations within certain DNA sequences, results in genetically diverese DNA molecules and genomes between individials.